Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,734,482	(T)5→6	coding (48/816 nt)	mepH →	peptidoglycan DD‑endopeptidase MepH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,734,477	1	.	T	100.0%	24.2 / NA	8	L15F (CTT→TTT)	mepH	peptidoglycan DD‑endopeptidase MepH
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (4/4);  total (4/4)

AGGCATGATAGACCTGCCTTTACAGAGGGACGCTCAGTGGCGCGGATAAACCGTATTTCGATCACGCTCTGTGCTTTAC‑TTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTACCACTCA  >  NC_000913/1734399‑1734550                                                                                |                                                                          aGGCATGATAGACCTGCCTTTACAGAGGGACGCTCAGTGGCGCGGATAAACCGTATTTCGATCACGCTCTGTGCTTTAC‑tt                                                                         >  1:281679/1‑81 (MQ=255)     aTGATAGACCTGCCTTTACAGAGGGACGCTCAGTGGCGCGGATAAACCGTATTTCGATCACGCTCTGTGCTTTAC‑ttttt                                                                      >  1:281689/1‑80 (MQ=255)             ccTGCCTTTACAGAGGGACGCTCAGTGGCGCGGATAAACCGTATTTCGATCACGCTCTGTGCTTTACTTTTTTACCACCCt                                                              <  2:281670/81‑1 (MQ=255)                  cTTTACAGAGGGACGCTCAGTGGCGCGGATAAACCGTATTTCGATCACGCTCTGTGCTTTACTTTTTTACCACCCTGCCtt                                                         <  1:281671/81‑1 (MQ=255)                       cAGAGGGACGCTCAGTGGCGCGGATAAACCGTATTTCGATCACGCTCTGTGCTTTACTTTTTTACCACCCTGCCTTTAACg                                                    <  1:281672/81‑1 (MQ=255)                        agagGGACGCTCAGTGGCGCGGATAAACCGTATTTCGATCACGCTCTGTGCTTTACTTTTTTACCACCCTGCCTTTAACGc                                                   >  1:281686/1‑81 (MQ=255)                                          gcgGATAAACCGTATTTCGATCACGCTCTGTGCTTTACTTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCaa                                 <  1:281673/81‑1 (MQ=255)                                                  aCCGTATTTCGATCACGCTCTGTGCTTTACTTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAaagcaagc                          >  1:281694/1‑80 (MQ=255)                                                                     ctGTGCTTTACTTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTACCa      >  1:281690/1‑81 (MQ=255)                                                                          cTTTACTTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTACCACTCa  >  2:281688/1‑80 (MQ=255)                                                                                |                                                                          AGGCATGATAGACCTGCCTTTACAGAGGGACGCTCAGTGGCGCGGATAAACCGTATTTCGATCACGCTCTGTGCTTTAC‑TTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTACCACTCA  >  NC_000913/1734399‑1734550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑