Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,063,976	(T)6→7	coding (492/1080 nt)	cobT ←	nicotinate‑nucleotide‑‑dimethylbenzimidazole phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,063,970	1	.	T	100.0%	72.8 / NA	21	N166K (AAC→AAA)	cobT	nicotinate‑nucleotide‑‑dimethylbenzimidazole phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (7/14);  total (7/14)

CAGTGATTGTGCTGACTATTGCTGCTGCCGGTGTCGTGTTTGCCATCCCCAGTTCACCTACACCAAACAGCGTGACACCG‑TTTTTTGCCAGCTCCTGCGTATAACATATGACGTCCAAAAGCAACTTTTCAGCCTGACGGCGACTCATTG  >  NC_000913/2063891‑2064040                                                                                 |                                                                       cAGTGATTGTGCTGACTATTGCTGCTGCCGGTGTCGTGTTTGCCATCCCCAGTTCACCTACACCAAACAGCGTGACACCG‑t                                                                       >  1:325404/1‑81 (MQ=255)         gtgCTGACTATTGCTGCTGCCGGTGTCGTGTTTGCCATCCCCAGTTCACCTACACCAAACAGCGTGACACCG‑tttttttgc                                                               >  2:325391/1‑78 (MQ=255)         gtgCTGACTATTGCTGCTGCCGGTGTCGTGTTTGCCATCCCCAGTTCACCTACACCAAACAGCGTGACACCG‑tttttttg                                                                <  1:325357/80‑3 (MQ=255)           gCTGACTATTGCTGCTGCCGGTGTCGTGTTTGCCATCCCCAGTTCACCTACACCAAACAGCGTGACACCG‑tttttttgc                                                               >  2:325392/1‑76 (MQ=255)               aCTATTGCTGCTGCCGGTGTCGTGTTTGCCATCCCCAGTTCACCTACACCAAACAGCGTGACACCGTTTTTTTGCCAGCTc                                                          >  2:325398/1‑81 (MQ=255)                   ttGCTGCTGCCGGTGTCGTGTTTGCCATCCCCAGTTCACCTACACCAAACAGCGTGACACCGTTTTTTTGCCAGCTCCt                                                        <  1:325358/79‑1 (MQ=255)                     gctgctgcCGGTGTCGTGTTTGCCATCCCCAGTTCACCTACACCAAACAGCGTGACACCGTTTTTTTGCCAGCTCCTGCg                                                     <  1:325359/80‑1 (MQ=255)                     gctgctgcCGGTGTCGTGTTTGCCATCCCCAGTTCACCTACACCAAACAGCGTGACACCGTTTTTTTGCCAGCTCCTGCg                                                     >  1:325397/1‑80 (MQ=255)                         ctgcCGGTGTCGTGTTTGCCATCCCCAGTTCACCTACACCAAACAGCGTGACACCGTTTTTTTGCCAGCTCCTGCGtat                                                  <  2:325360/79‑1 (MQ=255)                            ccGGTGTCGTGTTTGCCATCCCCAGTTCACCTACACCAAACAGCGTGACACCGTTTTTTTGCCAGCTCCTGCGTATAACat                                             <  1:325361/81‑1 (MQ=255)                            ccGGTGTCGTGTTTGCCATCCCCAGTTCACCTACACCAAACAGCGTGACACCGTTTTTTTGCCAGCTCCTGCGTATAACa                                              >  2:325396/1‑80 (MQ=255)                               gtgtCGTGTTTGCCATCCCCAGTTCACCTACACCAAACAGCGTGACACCGTTTTTTTGCCAGCTCCTGCGTATAACATATg                                          <  2:325362/81‑1 (MQ=255)                                  tCGTGTTTGCCATCCCCAGTTCACCTACACCAAACAGCGTGACACCGTTTTTTTGCCAGCTCCTGCGTATAACATATGACg                                       >  1:325400/1‑81 (MQ=255)                                          gCCATCCCCAGTTCACCTACACCAAACAGCGTGACACCGTTTTTTTGCCAGCTCCTGCGTATAACATATGACGTCCAAAAg                               >  1:325393/1‑81 (MQ=255)                                           ccATCCCCAGTTCACCTACACCAAACAGCGTGACACCGTTTTTTTGCCAGCTCCTGCGTATAACATATGACGTCCAAAAGc                              <  1:325363/81‑1 (MQ=255)                                                  cAGTTCACCTACACCAAACAGCGTGACACCGTTTTTTTGCCAGCTCCTGCGTATAACATATGACGTCCAAAAGCAACtttt                       <  2:325364/81‑1 (MQ=255)                                                   aGTTCACCTACACCAAACAGCGTGACACCGTTTTTTTGCCAGCTCCTGCGTATAACATATGACGTCCAAAAGCAACTTTTc                      <  2:325365/81‑1 (MQ=255)                                                     ttCACCTACACCAAACAGCGTGACACCGTTTTTTTGCCAGCTCCTGCGTATAACATATGACGTCCAAAAGCAACTTTTCAg                    <  2:325366/81‑1 (MQ=255)                                                       cACCTACACCAAACAGCGTGACACCGTTTTTTTGCCAGCTCCTGCGTATAACATATGACGTCCAAAAGCAACTTTTCAGc                   <  1:325367/80‑1 (MQ=255)                                                        aCCTACACCAAACAGCGTGACACCGTTTTTTTGCCAGCTCCTGCGTATAACATATGACGTCCAAAAGCAACTTTTCAGCCt                 <  2:325368/81‑1 (MQ=255)                                                            acacCAAACAGCGTGACACCGTTTTTTTGCCAGCTCCTGCGTATAACATATGACGTCCAAAAGCAACTTTTCAGCCTGACg             >  2:325410/1‑81 (MQ=255)                                                             cacCAAACAGCGTGACACCGTTTTTTTGCCAGCTCCTGCGTATAACATATGACGTCCAAAAGCAACTTTTCAGCCTGAc              <  2:325370/79‑1 (MQ=255)                                                             cacCAAACAGCGTGACACCGTTTTTTTGCCAGCTCCTGCGTATAACATATGACGTCCAAAAGCAACTTTTCAGCCTGACg             <  2:325369/80‑1 (MQ=255)                                                                      gCGTGACACCGTTTTTTTGCCAGCTCCTGCGTATAACATATGACGTCCAAAAGCAACTTTTCAGCCTGACGGCGACTCAtt   >  1:325399/1‑81 (MQ=255)                                                                       cGTGACACCGTTTTTTTGCCAGCTCCTGCGTATAACATATGACGTCCAAAAGCAACTTTTCAGCCTGACGGCGACTCATTg  <  2:325371/81‑1 (MQ=255)                                                                                 |                                                                       CAGTGATTGTGCTGACTATTGCTGCTGCCGGTGTCGTGTTTGCCATCCCCAGTTCACCTACACCAAACAGCGTGACACCG‑TTTTTTGCCAGCTCCTGCGTATAACATATGACGTCCAAAAGCAACTTTTCAGCCTGACGGCGACTCATTG  >  NC_000913/2063891‑2064040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑