Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,113,535	(T)7→8	coding (793/894 nt)	galF ←	UTP:glucose‑1‑phosphate uridylyltransferase, low activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,113,528	1	.	T	100.0%	81.8 / NA	23	M267K (ATG→AAG)	galF	UTP:glucose‑1‑phosphate uridylyltransferase, low activity
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (9/14);  total (9/14)

CTCAATACCTTTACGGAACTTCGCCCCTTCTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCA‑TTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGG  >  NC_000913/2113452‑2113600                                                                              |                                                                         ctcAATACCTTTACGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCA‑ttt                                                                       <  2:332303/80‑1 (MQ=255)        ccTTTACGGAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCA‑ttttttttgcc                                                               <  2:332304/81‑5 (MQ=255)                gAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTTGCCGCAGTCGt                                                        <  1:332305/81‑1 (MQ=255)                gAACTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTTGCCGCAGTCGt                                                        <  2:332306/81‑1 (MQ=255)                 aaCTTCGCCCCTTCCTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTTGCCGCAGTCGTa                                                       <  2:332307/81‑1 (MQ=255)                          ccttccttCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTTGCCGCAGTCGTAACTGTCGcc                                              <  1:332308/81‑1 (MQ=255)                           cttccttcAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTTGCCGCAGTCGTAACTGTCGCCg                                             >  2:332352/1‑81 (MQ=255)                           cttccttcAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTTGCCGCAGTCGTAACTGTCGCCg                                             >  1:332347/1‑81 (MQ=255)                                ttCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTTGCCGCAGTCGTAACTGTCGCCGGtcat                                        >  1:332339/1‑81 (MQ=255)                                  cAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTTGCCGCAGTCGTAACTGTCGCCGGtcatc                                       <  1:332309/80‑1 (MQ=255)                                   aGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAg                                     <  1:332310/81‑1 (MQ=255)                                        gCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCAtt                                 <  1:332311/80‑1 (MQ=255)                                         cGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCATTg                                >  1:332336/1‑80 (MQ=255)                                             ggCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCATTGCATc                            <  1:332312/80‑1 (MQ=255)                                             ggCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCATTGCATCa                           <  1:332313/81‑1 (MQ=255)                                               ccATACTTCACAAACGCCTGCATATAGCCCATTTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCATTGCATCAAc                         <  1:332314/81‑1 (MQ=255)                                                  tACTTCACAAACGCCTGCATATAGCCCATTTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCATTGCATCAACGGa                      >  2:332335/1‑81 (MQ=255)                                                   aCTTCACAAACGCCTGCATATAGCCCATTTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCATTGCATCAACGGAt                     >  2:332342/1‑81 (MQ=255)                                                       cacaAACGCCTGCATATAGCCCATTTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCATTGCATCAACGGATTGt                  <  1:332315/80‑1 (MQ=255)                                                        acaAACGCCTGCATATAGCCCATTTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCATTGCATCAACGGATTGttt                <  2:332316/81‑1 (MQ=255)                                                          aaaCGCCTGCATATAGCCCATTTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCATTGCATCAACGGATTGttttt              <  1:332317/81‑1 (MQ=255)                                                                  gCATATAGCCCATTTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGc      >  1:332350/1‑81 (MQ=255)                                                                     tataGCCCATTTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCg   <  1:332318/81‑1 (MQ=255)                                                                      ataGCCCATTTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCgg  >  1:332337/1‑81 (MQ=255)                                                                      ataGCCCATTTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCgg  >  2:332346/1‑81 (MQ=255)                                                                              |                                                                         CTCAATACCTTTACGGAACTTCGCCCCTTCTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCA‑TTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGG  >  NC_000913/2113452‑2113600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑