| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,125,056 | (T)7→8 | coding (691/1224 nt) | wcaI ← | putative colanic biosynthesis glycosyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,125,049 | 1 | . | T | 100.0% | 37.3 / NA | 12 | I233N (ATC→AAC) | wcaI | putative colanic biosynthesis glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base T (6/6); total (6/6) | |||||||||||
CATCGCGCAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGGAGTAAAGAATGA‑TTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACATGCTGAAAACGG > NC_000913/2124972‑2125123 | cATCGCGCAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGGAGTAAAGAATGA‑tt < 1:334178/80‑1 (MQ=255) aTCGCGCAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGGAGTAAAGAATGA‑tttt < 1:334180/81‑1 (MQ=255) aTCGCGCAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGGAGTAAAGAATGA‑ttt < 1:334179/80‑1 (MQ=255) cgcgCAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGGAGTAAAGAATGA‑tttttt > 1:334220/1‑81 (MQ=255) cgcAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGGAGTAAAGAATGA‑tttttttt < 2:334181/81‑2 (MQ=255) gcAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGGAGTAAAGAATGA‑ttttttttg < 2:699457/81‑3 (MQ=255) aGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGGAGTAAAGAATGA‑ttttttttgt > 1:334212/1‑77 (MQ=255) tAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGGAGTAAAGAATGATTTTTTTTGTTATCCGGCAGGTCAAGCTgg > 2:334225/1‑81 (MQ=255) cGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGGAGTAAAGAATGATTTTTTTTGTTATCCGGCAGGTCAAGCTGGTTa < 2:334182/81‑1 (MQ=255) cGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGGAGTAAAGAATGATTTTTTTTGTTATCCGGCAGGTCAAGCTGGTTa > 2:334209/1‑81 (MQ=255) ttCCAGCCCCTGCTTTTCACCAATATTGCCGGAGTAAAGAATGATTTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGa < 1:334183/80‑1 (MQ=255) ttCCAGCCCCTGCTTTTCACCAATATTGCCGGAGTAAAGAATGATTTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGa < 2:334184/80‑1 (MQ=255) ccTGCTTTTCACCAATATTGCCGGAGTAAAGAATGATTTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCa < 1:334185/81‑1 (MQ=255) ccTGCTTTTCACCAATATTGCCGGAGTAAAGAAAGATTTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATc > 1:334213/1‑80 (MQ=255) cTTTTCACCAATATTGCCGGAGTAAAGAATGATTTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACAt > 2:334217/1‑81 (MQ=255) cACCAATATTGCCGGAGTAAAGAATGATTTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGc < 1:334186/80‑1 (MQ=255) ttGCCGGAGTAAAGAATGATTTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAAc < 2:334187/81‑1 (MQ=255) aGTAAAGAATGATTTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACATGCTg > 1:334216/1‑80 (MQ=255) gAATGATTTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACATGCTGAAAACgg > 1:334223/1‑81 (MQ=255) | CATCGCGCAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGGAGTAAAGAATGA‑TTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACATGCTGAAAACGG > NC_000913/2124972‑2125123 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |