Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,312,788	(T)6→7	intergenic (‑39/‑296)	ompC ← / → micF	outer membrane porin C/small regulatory RNA MicF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,312,782	1	.	T	100.0%	47.0 / NA	14	intergenic (‑33/‑302)	ompC/micF	outer membrane porin C/small regulatory RNA MicF
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (4/10);  total (4/10)

CCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGC‑TTTTTTATGCCACTGCATACTGATTAACCCTCATTAATCAGTCGGCAAGTCCATTCTCCCCAA  >  NC_000913/2312707‑2312845                                                                             |                                                                ccAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGC‑ttt                                                              <  2:364055/79‑1 (MQ=255)  cagcagAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGC‑tttttt                                                           <  1:364056/81‑1 (MQ=255)  cagcagAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGC‑tttttt                                                           >  1:364071/1‑81 (MQ=255)        agCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCTTTTTTTATGcc                                                      <  1:364058/81‑1 (MQ=255)        agCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCTTTTTTTATGcc                                                      <  2:364057/81‑1 (MQ=255)         gCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCTTTTTTTATGCCa                                                     <  2:364059/81‑1 (MQ=255)         gCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCTTTTTTTATGCCa                                                     <  2:364060/81‑1 (MQ=255)          cTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCTTTTTTTATGCCAc                                                    <  2:364061/81‑1 (MQ=255)           tGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCTTTTTTTATGCCACt                                                   >  2:364082/1‑81 (MQ=255)                  aggaggGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCTTTTTTTATGCCACTGCATACt                                            >  2:364090/1‑81 (MQ=255)                   ggaggGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCTTTTTTTATGCCACTGCATACTg                                           <  1:364062/81‑1 (MQ=255)                        gACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCTTTTTTTATGCCACTGCATACTGATTaa                                      <  1:364063/81‑1 (MQ=255)                        gACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCTTTTTTTATGCCACTGCATACTGATTaa                                      >  2:364093/1‑81 (MQ=255)                               cTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCTTTTTTTATGCCACTGCATACTGATTAACCCTCa                                <  2:364064/80‑1 (MQ=255)                                        tCATGTTATTAACCCTCTGTTATATGCCTTTATTTGCTTTTTTTATGCCACTGCATACTGATTAACCCTCATTAATCAGt                       <  1:364065/80‑1 (MQ=255)                                         cATGTTATTAACCCTCTGTTATATGCCTTTATTTGCTTTTTTTATGCCACTGCATACTGATTAACCCTCATTAATCAGTc                      <  2:364066/80‑1 (MQ=255)                                                             atatGCCTTTATTTGCTTTTTTTATGCCACTGCATACTGATTAACCCTCATTAATCAGTCGGCAAGTCCATTCTCCCCaa  >  1:364088/1‑80 (MQ=255)                                                                             |                                                                CCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTTTCATGTTATTAACCCTCTGTTATATGCCTTTATTTGC‑TTTTTTATGCCACTGCATACTGATTAACCCTCATTAATCAGTCGGCAAGTCCATTCTCCCCAA  >  NC_000913/2312707‑2312845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑