| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,328,408 | (T)7→8 | coding (1390/1650 nt) | yfaQ ← | tandem DUF2300 domain‑containing protein YfaQ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,328,401 | 1 | . | T | 93.3% | 46.3 / ‑3.1 | 15 | coding (1397/1650 nt) | yfaQ | tandem DUF2300 domain‑containing protein YfaQ |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base T (11/3); total (11/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGAGACTAAACGGCAAACCGCAAACACGTCTGGTTCGTTGTACCCCGTCTCAGCTTGTAATATACGCCGCCACTGCGGCA‑TT‑TTTTTCGCCAGCCAGGCTTTCGCTTTGGGTAATAACTGGCAGGTTGAGCGTGGCGCTCCCCAGCGGCTAAGAC > NC_000913/2328321‑2328476 | cTGAGACTAAACGGCAAACCGCAAACACGTCTGGTTCGTTGTACCCCGTCTCAGCTTGTAATATACGCCGCCACTGCGGCa < 2:366646/81‑1 (MQ=255) agaCTAAACGGCAAACCGCAAACACGTCTGGTTCGTTGTACCCCGTCTCAGCTTGTAATATACGCCGCCACTGCGGCA‑ttc < 2:366647/81‑2 (MQ=255) aaaCGGCAAACCGCAAACACGTCTGGTTCGTTGTACCCCGTCTCAGCTTGTAATATACGCCGCCACTGCGGCA‑tt‑tttttt > 2:366661/1‑80 (MQ=255) aaCGGCAAACCGCAAACACGTCTGGTTCGTTGTACCCCGTCTCAGCTTGTAATATACGCCGCCACTGCGGCA‑tt‑ttttttc < 1:366648/81‑3 (MQ=255) aaCGGCAAACCGCAAACACGTCTGGTTCGTTGTACCCCGTCTCAGCTTGTAATATACGCCGCCACTGCGGCA‑tt‑ttttttc > 1:366675/1‑79 (MQ=255) ggCAAACCGCAAACACGTCTGGTTCGTTGTACCCCGTCTCAGCTTGTAATATACGCCGCCACTGCGGCA‑tt‑ttttttcgc > 1:366676/1‑76 (MQ=255) ccGCAAACACGTCTGGTTCGTTGTACCCCGTCTCAGCTTGTAATATACGCCGCCACTGCGGCATTT‑TTTTTCGccagccag > 1:366672/1‑81 (MQ=255) cAAACACGTCTGGTTCGTTGTACCCCGTCTCAGCTTGTAATATACGCCGCCACTGCGGCA‑TTGTTATTCGCCAGCCAGGCt < 2:366649/81‑1 (MQ=255) cAAACACGTCTGGTTCGTTGTACCCCGTCTCAGCTTGTAATATACGCC‑CCACTGCGGCATTT‑TTTTTCGCCAGCCAGGCtt > 1:366679/1‑81 (MQ=255) aaCACGTCTGGTTCGTTGTACCCCGTCTCAGCTTGTAATATACGCCGCCACTGCGGCATTT‑TTTTTCGCCAGCCAGGCttt > 2:366683/1‑81 (MQ=255) tGGTTCGTTGTACCCCGTCTCAGCTTGTAATATACGCCGCCACTGCGGCATTT‑TTTTTCGCCAGCCAGGCTTTCGCTTTgg > 2:366684/1‑81 (MQ=255) tGGTTCGTTGTACCCCGTCTCAGCTTGTAATATACGCCGCCACTGCGGCATTT‑TTTTTCGCCAGCCAGGCTTTCGCTTTgg > 1:366680/1‑81 (MQ=255) gTCTCAGCTTGTAATATACGCCGCCACTGCGGCATTT‑TTTTTCGCCAGCCAGGCTTTCGCTTTGGGTAATAACTGGCAGGt > 1:366673/1‑81 (MQ=255) tAATATACGCCGCCACTGCGGCATTT‑TTTTTCGCCAGCCAGGCTTTCGCTTTGGGTAATAACTGGCAGGTTGAGCGTGgc < 2:366650/80‑1 (MQ=255) aaTATACGCCGCCACTGCGGCATTT‑TTTTTCGCCAGCCAGGCTTTCGCTTTGGGTAATAACTGGCAGGTTGAGCGTGgcgc > 1:366689/1‑81 (MQ=255) ataCGCCGCCACTGCGGCATTT‑TTTTTCGCCAGCCAGGCTTTCGCTTTGGGTAATAACTGGCAGGTTGAGCGTGGCGCTcc > 1:366688/1‑81 (MQ=255) cgacgccACTGCGGCATTT‑TTTTTCGCCAGCCAGGCTTTCGCTTTGGGTAATAACTGGCAGGTTGAGCGTGGCGCTcccc < 2:366651/77‑1 (MQ=255) cTGCGGCATTT‑TTTTTCGCCAGCCAGGCTTTCGCTTTGGGTAATAACTGGCAGGTTGAGCGTGGCGCTCCCCAGCGGCTaa < 2:366652/81‑1 (MQ=255) gCGGCATTT‑TTTTTCGCCAGCCAGGCTTTCGCTTTGGGTAATAACTGGCAGGTTGAGCGTGGCGCTCCCCAGCGGCTAAGa > 1:366697/1‑81 (MQ=255) gCGGCATTT‑TTTTTCGCCAGCCAGGCTTTCGCTTTGGGTAATAACTGGCAGGTTGAGCGTGGCGCTCCCCAGCGGCTAAGa > 2:366681/1‑81 (MQ=255) cGGCATTT‑TTTTTCGCCAGCCAGGCTTTCGCTTTGGGTAATAACTGGCAGGTTGAGCGTGGCGCTCCCCAGCGGCTAAGAc > 1:366686/1‑81 (MQ=255) | CTGAGACTAAACGGCAAACCGCAAACACGTCTGGTTCGTTGTACCCCGTCTCAGCTTGTAATATACGCCGCCACTGCGGCA‑TT‑TTTTTCGCCAGCCAGGCTTTCGCTTTGGGTAATAACTGGCAGGTTGAGCGTGGCGCTCCCCAGCGGCTAAGAC > NC_000913/2328321‑2328476 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |