Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,602,017	(C)7→8	coding (200/2019 nt)	hyfB →	hydrogenase 4 component B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,602,010	1	.	C	100.0%	81.2 / NA	24	A65P (GCC→CCC)	hyfB	hydrogenase 4 component B
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (13/11);  total (13/11)

TCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCT  >  NC_000913/2601932‑2602082                                                                                |                                                                         tCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑cc                                                                        <  1:414121/81‑1 (MQ=255)   gCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑cccc                                                                      >  1:414156/1‑81 (MQ=255)   gCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑ccc                                                                       <  1:414122/80‑1 (MQ=255)             ggcgTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGa                                                             <  1:414123/81‑1 (MQ=255)                    ttGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGcc                                                      <  1:414124/81‑1 (MQ=255)                      ggCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCg                                                     <  2:414125/80‑1 (MQ=255)                            atcaGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAAc                                              >  1:414160/1‑81 (MQ=255)                               aGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCt                                           >  2:414165/1‑81 (MQ=255)                                  ggAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGc                                         >  1:414154/1‑80 (MQ=255)                                  ggAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCg                                        <  1:414126/81‑1 (MQ=255)                                         cGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGa                                  <  2:414128/80‑1 (MQ=255)                                         cGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGAt                                 <  1:414127/81‑1 (MQ=255)                                           cAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAg                               >  2:414161/1‑81 (MQ=255)                                           cAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAg                               <  2:414129/81‑1 (MQ=255)                                             aTTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGcc                             >  1:414159/1‑81 (MQ=255)                                              ttATATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCct                            >  1:414166/1‑81 (MQ=255)                                              ttACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCct                            >  2:414163/1‑81 (MQ=255)                                                 cATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCtctc                         >  2:414168/1‑81 (MQ=255)                                                        gtgtAACTTTAGTCGCCCGTTTTGCCCCCCCCTCTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCAt                    <  1:414130/79‑1 (MQ=255)                                                           tAACTTTAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTAt                <  2:414131/80‑1 (MQ=255)                                                               tttAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGc           >  2:414180/1‑81 (MQ=255)                                                                ttAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCt          >  2:414171/1‑81 (MQ=255)                                                                 tAGTCGCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGc           <  1:414132/79‑1 (MQ=255)                                                                      gCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGTTTTAACCCTGCGAATGGATAGCCTCCCGGCATTTATGGTGCTGGTTAt    >  2:414169/1‑81 (MQ=255)                                                                      gCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTa     <  1:414133/80‑1 (MQ=255)                                                                      gCCCGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTAt    >  1:414178/1‑81 (MQ=255)                                                                        ccGTTTTGCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTAtct  >  1:414167/1‑81 (MQ=255)                                                                                |                                                                         TCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCT  >  NC_000913/2601932‑2602082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑