Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,763,869	(T)7→8	coding (1285/1617 nt)	abpA ←	CP4‑57 prophage; anti‑bacteriophage protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,763,862	1	.	T	100.0%	64.0 / NA	19	I431N (ATC→AAC)	abpA	CP4‑57 prophage; anti‑bacteriophage protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (9/10);  total (9/10)

CGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGA‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCT  >  NC_000913/2763788‑2763932                                                                            |                                                                       cGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGA‑tttttt                                                                  >  1:445153/1‑81 (MQ=255)  gAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGA‑ttttttt                                                                 >  1:445160/1‑81 (MQ=255)      ggTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGA‑ttttttttgcg                                                             <  1:445111/81‑5 (MQ=255)            cTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTTGCGCGCGGt                                                        <  1:445112/81‑1 (MQ=255)            cTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTTGCGCGCGGt                                                        <  2:445113/81‑1 (MQ=255)                cATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCg                                                    <  1:445114/81‑1 (MQ=255)                        aTTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATc                                            <  2:445115/81‑1 (MQ=255)                          ttCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATcc                                           >  2:445161/1‑80 (MQ=255)                           tCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCg                                          <  2:445116/80‑1 (MQ=255)                                  tGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTcc                                  <  2:445117/81‑1 (MQ=255)                                      gTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCg                               >  2:445159/1‑80 (MQ=255)                                      gTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCg                               >  2:445166/1‑80 (MQ=255)                                           gAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAAc                         >  1:445155/1‑81 (MQ=255)                                           gAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAAc                         >  1:445167/1‑81 (MQ=255)                                           gAAGCAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAAc                         >  2:445162/1‑81 (MQ=255)                                               cAGGTAGTTATCGTCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGAt                     <  2:445118/81‑1 (MQ=255)                                                   tAGTTATCGTCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCg                 >  1:445172/1‑81 (MQ=255)                                                     gTTATCGTCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTa               <  1:445119/81‑1 (MQ=255)                                                      ttATCGTCTTTAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTAt              <  2:445120/81‑1 (MQ=255)                                                                tAATGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAAt    >  2:445156/1‑81 (MQ=255)                                                                  aTGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATc   <  1:445121/80‑1 (MQ=255)                                                                  aTGTCGAGGATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCt  >  2:445154/1‑81 (MQ=255)                                                                            |                                                                       CGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGA‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCT  >  NC_000913/2763788‑2763932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑