Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,947,713	(T)7→8	intergenic (+30/+44)	metV → / ← amiC	tRNA‑Met/N‑acetylmuramoyl‑L‑alanine amidase C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,947,706	1	.	T	100.0%	72.4 / NA	21	intergenic (+23/+51)	metV/amiC	tRNA‑Met/N‑acetylmuramoyl‑L‑alanine amidase C
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (8/13);  total (8/13)

AGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTG‑TTTTTTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGCCAGCGTCG  >  NC_000913/2947628‑2947779                                                                                |                                                                          aGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTG‑tt                                                                         >  2:481010/1‑81 (MQ=255)      gtcgGGATCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTG‑ttttttt                                                                    <  2:480989/81‑1 (MQ=255)         gggCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTG‑ttttttttgt                                                                 <  1:480990/81‑4 (MQ=255)          ggCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTG‑ttttttttgtt                                                                <  1:480991/81‑5 (MQ=255)              cATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTTGTTTCTg                                                             >  2:481023/1‑81 (MQ=255)              cATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTTGTTTCTg                                                             >  2:481022/1‑81 (MQ=255)              cATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTTGTTTCTg                                                             >  1:481020/1‑81 (MQ=255)                  aCCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTTGTTTCTGGtct                                                         <  1:480992/81‑1 (MQ=255)                   cccGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTTGTTTCTGGtctc                                                        <  2:480993/81‑1 (MQ=255)                            cgtcgGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTTGTTTCTGGTCTCCCATaaaa                                                >  1:481024/1‑80 (MQ=255)                                ggTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTTGTTTCTGGTCTCCCATAAAAAAgcg                                           >  2:481021/1‑81 (MQ=255)                                  ttCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTTGTTTCTGGTCTCCCATAAAAAAGCGcc                                         <  1:480994/81‑1 (MQ=255)                                    cAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTTGTTTCTGGTCTCCCATAAAAAAGCGCCa                                        <  2:480995/80‑1 (MQ=255)                                     aaaTCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTTGTTTCTGGTCTCCCATAAAAAAGCGCCAtt                                      <  1:480996/81‑1 (MQ=255)                                      aaTCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTc                                     <  2:480998/81‑1 (MQ=255)                                      aaTCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTc                                     <  1:480997/81‑1 (MQ=255)                                        tCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAg                                   <  2:480999/81‑1 (MQ=255)                                          cGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAgc                                  <  2:481000/80‑1 (MQ=255)                                          cGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAgc                                  <  1:481001/80‑1 (MQ=255)                                           ggCCCCCGCAACCAATTATTGAACACCCTAACGGGTGTTTTTTTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAgcgc                                >  2:481028/1‑81 (MQ=255)                                                                  cacCCTAACGGGTGTTTTTTTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGcc         <  1:481002/81‑1 (MQ=255)                                                                      cTAACGGGTGTTTTTTTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGCCAGCg     >  2:481031/1‑81 (MQ=255)                                                                      cTAACGGGTGTTTTTTTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGCCAGCg     >  2:481044/1‑81 (MQ=255)                                                                         aCGGGTGTTTTTTTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGCCAGCGTc   <  2:481004/80‑1 (MQ=255)                                                                         aCGGGTGTTTTTTTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGCCAGCGTCg  <  2:481003/81‑1 (MQ=255)                                                                                |                                                                          AGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCTAACGGGTG‑TTTTTTTGTTTCTGGTCTCCCATAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGCCAGCGTCG  >  NC_000913/2947628‑2947779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑