| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,060,772 | (T)8→9 | intergenic (+126/‑78) | argP → / → scpA | DNA‑binding transcriptional dual regulator ArgP/methylmalonyl‑CoA mutase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,060,764 | 1 | . | T | 96.7% | 103.2 / ‑3.2 | 30 | intergenic (+118/‑86) | argP/scpA | DNA‑binding transcriptional dual regulator ArgP/methylmalonyl‑CoA mutase |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base T (15/14); total (15/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
CCTCACTTCTTTTTGTATTCCTTGAATCACATCACAAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCA‑‑TTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGGCGCAAATACCCTCATTTTGATTGCGTTTTAC > NC_000913/3060686‑3060839 | ccTCACTTCTTTTTGTATTCCTTGAATCACATCACAAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCA‑‑tt > 1:503833/1‑81 (MQ=255) aCTTCTTTTTGTATTCCTTGAATCACATCACAAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCA‑‑tttt < 1:503800/79‑1 (MQ=255) tttGTATTCCTTGAATCACATCACAAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCA‑‑tttttttttg < 2:503800/78‑3 (MQ=255) ttCCTTGAATCACATCACAAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCAT‑TTTTTTTTGCTGGCGAc < 1:503802/80‑1 (MQ=255) ttCCTTGAATCACATCACAAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCAT‑TTTTTTTTGCTGGCGAc > 2:503830/1‑80 (MQ=255) ccTTGAATCACATCACAAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCAT‑TTTTTTTTGCTGGCGACaa < 2:503802/80‑1 (MQ=255) cTTGAATCACATCACAAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCAT‑TTTTTTTTGCTGGCGACAAAc > 1:503845/1‑81 (MQ=255) tGAATCACATCACAAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCAT‑TTTTTTTTGCTGGCGACAAAcc > 2:503828/1‑80 (MQ=255) aaTCACATCACAAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCAT‑TTTTTTTTGCTGGCGACAAACCCAc < 2:503803/81‑1 (MQ=255) tcacatcacaAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCAT‑TTTTTTTTGCTGGCGACAAACCCACGt < 2:503804/81‑1 (MQ=255) cacatcacaAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCATTTTTTTTTTGCTGGCGACAAACCCACg > 2:503839/1‑80 (MQ=255) cacatcacaAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCAT‑TTTTTTTTGCTGGCGACAAACCCACGt > 2:503831/1‑80 (MQ=255) acatcacaAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCAT‑TTTTTTTTGCTGGCGACAAACCCACGTaa < 1:503806/81‑1 (MQ=255) acaAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCAT‑TTTTTTTTGCTGGCGACAAACCCACGTAAAAAg < 2:503806/80‑1 (MQ=255) aaaaTAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCAT‑TTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTc < 1:503808/81‑1 (MQ=255) gACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCA‑‑TTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAg < 1:503809/81‑1 (MQ=255) aaaTCTCAGGCGGCAAAAAACGACGTCTGAATGCAT‑TTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGgc < 1:503810/81‑1 (MQ=255) aTCTCAGGCGGCAAAAAACGACGTCTGAATGCAT‑TTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGgcgc < 2:503810/81‑1 (MQ=255) tcAGGCGGCAAAAAACGACGTCTGAATGCAT‑TTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGGCGCaaa > 1:503843/1‑81 (MQ=255) cAGGCGGCAAAAAACGACGTCTGAATGCAT‑TTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGGCGCaaa > 2:503833/1‑80 (MQ=255) aGGCGGCAAAAAACGACGTCTGAATGCAT‑TTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGGCGCaa < 1:503812/78‑1 (MQ=255) gcAAAAAACGACGTCTGAATGCAT‑TTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGGCGCAAATACCCTc < 1:503813/81‑1 (MQ=255) acgacgTCTGAATGCAT‑TTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGGCGCAAATACCCTCATTTTGa > 1:503836/1‑81 (MQ=255) cgacgTCTGAATGCAT‑TTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGGCGCAAATACCCTCATTTTGAt > 2:503836/1‑81 (MQ=255) gacgTCTGAATGCAT‑TTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGGCGCAAATACCCTCATTTTGAtt > 2:503841/1‑81 (MQ=255) gacgTCTGAATGCAT‑TTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGGCGCAAATACCCTCATTTTGAtt > 2:503846/1‑81 (MQ=255) tCTGAATGCAT‑TTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGGCGCAAATACCCTCATTTTGATTGCGt < 2:503813/81‑1 (MQ=255) tCTGAATGCAT‑TTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGGCGCAAATACCCTCATTTTGATTGCGt > 2:503838/1‑81 (MQ=255) aaTGCAT‑TTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGGCGCAAATACCCTCATTTTGATTGCGtttt < 2:503815/80‑1 (MQ=255) aaTGCAT‑TTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGGCGCAAATACCCTCATTTTGATTGCGttt < 1:503817/79‑1 (MQ=255) aaTGCAT‑TTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGGCGCAAATACCCTCATTTTGATTGCGTTTTa > 1:503844/1‑81 (MQ=255) aaTGCAT‑TTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGGCGCAAATACCCTCATTTTGATTGCGTTTTa > 2:503848/1‑81 (MQ=255) aTGCAT‑TTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGGCGCAAATACCCTCATTTTGATTGCGTTTTAc > 1:503838/1‑81 (MQ=255) | CCTCACTTCTTTTTGTATTCCTTGAATCACATCACAAAATAGACAAATCTCAGGCGGCAAAAAACGACGTCTGAATGCA‑‑TTTTTTTTGCTGGCGACAAACCCACGTAAAAAGCTCACCGTAGGCGCAAATACCCTCATTTTGATTGCGTTTTAC > NC_000913/3060686‑3060839 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |