Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,586,418	(T)6→7	coding (406/1743 nt)	ggt ←	glutathione hydrolase proenzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,586,412	1	.	T	100.0%	108.1 / NA	30	S138T (TCA→ACA)	ggt	glutathione hydrolase proenzyme
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (8/22);  total (8/22)

GCCGTATTTATCCAGCGCCAGCGAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGA‑TTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGCCATTTCGCGG  >  NC_000913/3586338‑3586485                                                                            |                                                                          gCCGTATTTATCCAGCGCCAGCGAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGA‑tttttt                                                                     >  2:625710/1‑81 (MQ=255)  ccGTATTTATCCAGCGCCAGCGAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGA‑ttttttt                                                                    <  2:625637/81‑2 (MQ=255)    gTATTTATCCAGCGCCAGCGAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGA‑tttttttgc                                                                  <  1:625639/81‑4 (MQ=255)    gTATTTATCCAGCGCCAGCGAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGA‑tttttttgc                                                                  >  1:625708/1‑78 (MQ=255)    gTATTTATCCAGCGCCAGCGAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGA‑tttttttgc                                                                  >  1:625696/1‑78 (MQ=255)    gTATTTATCCAGCGCCAGCGAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGCGA‑tttttttgc                                                                  >  1:625700/1‑78 (MQ=255)           tCCAGCGCCAGCGAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTTGCTGTcccg                                                            >  2:625697/1‑79 (MQ=255)           tCCAGCGCCAGCGAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTTGCTGTCCg                                                             <  2:625639/80‑1 (MQ=255)              agcgccagcgAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTTGCTGTCCGGGtt                                                         <  1:625641/81‑1 (MQ=255)                   cagcgAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTTGCTGTCCGGGTTGCCCt                                                    <  1:625642/81‑1 (MQ=255)                   cagcgAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTTGCTGTCCGGGTTGCCCt                                                    >  1:625718/1‑81 (MQ=255)                      cgAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTTGCTGTCCGGGTTGCCCTGat                                                 <  2:625642/81‑1 (MQ=255)                                    ccGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACata                                   <  2:625643/81‑1 (MQ=255)                                    ccGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACat                                    <  2:625646/80‑1 (MQ=255)                                    ccGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACat                                    <  2:625644/80‑1 (MQ=255)                                    ccGTACCCGGTGTGCCGGAAGCCAGATGCAAAGTGAGTGATTTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACa                                     <  2:625645/79‑1 (MQ=255)                                     cGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACatat                                  <  1:625648/81‑1 (MQ=255)                                      gTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACATATc                                 <  2:625649/81‑1 (MQ=255)                                      gTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACATATc                                 <  2:625648/81‑1 (MQ=255)                                        aCCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACATATcgc                               >  2:625735/1‑81 (MQ=255)                                        aCCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACATATcgc                               >  2:625721/1‑81 (MQ=255)                                        aCCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACATATcgc                               >  1:625712/1‑81 (MQ=255)                                            ggTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACATATCGCGGGt                           <  1:625651/81‑1 (MQ=255)                                            ggTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACATATCGCGGGt                           <  2:625651/81‑1 (MQ=255)                                                  ccGGAAGCCAGATGCGAAGTGAGTGATTTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACATATCGCGGGTCGCttt                     <  1:625655/81‑1 (MQ=255)                                                  ccGGAAGCCAGATGCGAAGTGAGTGATTTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACATATCGCGGGTCGCttt                     <  2:625652/81‑1 (MQ=255)                                                  ccGGAAGCCAGATGCGAAGTGAGTGATTTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACATATCGCGGGTCGCttt                     <  2:625653/81‑1 (MQ=255)                                                  ccGGAAGCCAGATGCGAAGTGAGTGATTTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACATATCGCGGGTCGCttt                     >  2:625702/1‑81 (MQ=255)                                                   cGGAAGCCAGATGCGAAGTGAGTGATTTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTg                    <  1:625656/81‑1 (MQ=255)                                                     gAAGCCAGATGCGAAGTGAGTGATTTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGc                  <  1:625657/81‑1 (MQ=255)                                                           aGATGCGAAGTGAGTGATTTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGc            >  1:625714/1‑81 (MQ=255)                                                            gATGCGAAGTGAGTGATTTTTTTGCTGTCCGGGTTGCTCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGcc           <  2:625657/81‑1 (MQ=255)                                                                  aaGTGAGTGATTTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGCCATTTcg     <  2:625658/81‑1 (MQ=255)                                                                  aaGTGAGTGATTTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGCCATTTcg     >  2:625712/1‑81 (MQ=255)                                                                   agtgagtgATTTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGCCATTTcgc    <  1:625660/81‑1 (MQ=255)                                                                   agtgagtgATTTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGCCATTTcgc    <  1:625661/81‑1 (MQ=255)                                                                      gagtgatTTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGCCATTTCGCgg  >  1:625727/1‑80 (MQ=255)                                                                            |                                                                          GCCGTATTTATCCAGCGCCAGCGAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGA‑TTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGCCATTTCGCGG  >  NC_000913/3586338‑3586485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑