Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,950,476	(T)8→9	intergenic (+56/‑31)	ilvL → / → ilvX	ilvXGMEDA operon leader peptide/uncharacterized protein IlvX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,950,468	1	.	T	100.0%	87.9 / NA	25	intergenic (+48/‑39)	ilvL/ilvX	ilvXGMEDA operon leader peptide/uncharacterized protein IlvX
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (12/13);  total (12/13)

GCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTC  >  NC_000913/3950394‑3950542                                                                            |                                                                           gCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑tttttt                                                                      <  2:720555/81‑1 (MQ=255)      aCTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑tttttttttga                                                                 >  2:720602/1‑78 (MQ=255)      aCTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑tttttttttga                                                                 >  1:720600/1‑78 (MQ=255)           gACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTTGACCtt                                                              <  2:720557/80‑1 (MQ=255)           gACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTTGACCTTa                                                             <  2:720556/81‑1 (MQ=255)             cGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTTGACCTTaaa                                                           <  1:720559/81‑1 (MQ=255)               aGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTTGACCTTaaaaa                                                         >  1:720588/1‑81 (MQ=255)                ggAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTTGACCTTaaaaa                                                         >  1:720590/1‑80 (MQ=255)                  aaaGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTTGACCTTAAAAACAt                                                      <  1:720560/81‑1 (MQ=255)                   aaGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTTGACCTTAAAAACAt                                                      >  1:720592/1‑80 (MQ=255)                   aaGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTTGACCTTAAAAACAt                                                      >  1:720593/1‑80 (MQ=255)                   aaGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTTGACCTTAAAAACAt                                                      >  2:720601/1‑80 (MQ=255)                    aGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTTGACCTTAAAAACATaa                                                    >  2:720596/1‑81 (MQ=255)                                 aaGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGa                                        >  2:720597/1‑80 (MQ=255)                                 aaGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGAc                                       <  1:720561/81‑1 (MQ=255)                                    ccTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAAt                                    <  1:720562/81‑1 (MQ=255)                                           gAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATaa                              >  2:720611/1‑80 (MQ=255)                                              cTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGc                          <  1:720564/81‑1 (MQ=255)                                              cTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGc                          <  2:720562/81‑1 (MQ=255)                                                   aCCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACaaa                     <  1:720565/81‑1 (MQ=255)                                                     ccccGCACCGAAAGGTCCGGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAAt                   <  1:720566/81‑1 (MQ=255)                                                       ccGCACCGAAAGGTCCGGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTc                 <  2:720566/81‑1 (MQ=255)                                                           aCCGAAAGGTCCGGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGtt             <  1:720568/81‑1 (MQ=255)                                                              gAAAGGTCCGGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTtct          >  2:720600/1‑81 (MQ=255)                                                              gAAAGGTCCGGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTtct          >  1:720615/1‑81 (MQ=255)                                                               aaaGGTCCGGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTtct          >  2:720604/1‑80 (MQ=255)                                                                   gTCCGGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCATACAATGAATAACAGCACAAAATTCTGTTTCTCAAGa     <  2:720568/81‑1 (MQ=255)                                                                   gTCCGGGGGTTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTtctc         >  1:720604/1‑77 (MQ=255)                                                                     cgggggt‑TTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGAtt   <  2:720569/78‑1 (MQ=255)                                                                     cgggggt‑TTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGAtt   <  1:720571/78‑1 (MQ=255)                                                                     cgggggt‑TTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTc  <  2:720571/79‑1 (MQ=255)                                                                      gggggt‑TTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGAtt   >  2:720605/2‑79 (MQ=255)                                                                            |                                                                           GCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTC  >  NC_000913/3950394‑3950542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑