Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	77,593	(T)8→9	noncoding (227/227 nt)	sgrS →	small regulatory RNA SgrS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	77,585	1	.	T	100.0%	84.1 / NA	24	noncoding (219/227 nt)	sgrS	small regulatory RNA SgrS
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (13/11);  total (13/11)

TTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTG  >  NC_000913/77507‑77660                                                                                |                                                                            ttCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑tt                                                                           <  2:17259/81‑1 (MQ=255)       aaGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑tttttttt                                                                     <  2:17260/81‑1 (MQ=255)          ttGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑tttttttttat                                                                  <  1:17261/81‑4 (MQ=255)              cTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTTATtctc                                                               <  1:17263/81‑1 (MQ=255)                       atcatGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTTATTCTCGCCGCGCTa                                                      <  2:17265/81‑1 (MQ=255)                       atcatGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTTATTCTCGCCGCGCTa                                                      <  2:17264/81‑1 (MQ=255)                         catGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTTATTCTCGCCGCGCTaa                                                     >  2:17291/1‑80 (MQ=255)                          atGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTTATTCTCGCCGCGCTaaa                                                    >  2:124705/1‑80 (MQ=255)                                     aGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTAt                                        <  2:17266/81‑1 (MQ=255)                                         ttGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATc                                    <  1:17267/81‑1 (MQ=255)                                               tAAAATCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGAt                               <  1:17268/80‑1 (MQ=255)                                                 aaaTCACCCGCCAGCAGATTATACCTGCTGGTTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAAt                            >  1:17299/1‑81 (MQ=255)                                                             agcagATTATACCTGCTGGTTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCg                >  1:17312/1‑81 (MQ=255)                                                              gcagATTATACCTGCTGGTTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGc               >  1:17303/1‑81 (MQ=255)                                                                agATTATACCTGCTGGTTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCg             <  1:17269/81‑1 (MQ=255)                                                                 gATTATACCTGCTGGTTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCg             >  2:17307/1‑80 (MQ=255)                                                                  aTTATACCTGCTGGTTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTa           >  1:17297/1‑81 (MQ=255)                                                                   ttATACCTGCTGGTTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTAt          <  2:17270/81‑1 (MQ=255)                                                                    tataCCTGCTGGTTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTAt          >  2:17301/1‑80 (MQ=255)                                                                    tataCCTGCTGGTTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTAt          >  2:17305/1‑80 (MQ=255)                                                                      taactgctgGTTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGaa       <  2:17271/78‑1 (MQ=255)                                                                      taCCTGCTGGTTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGaa       <  2:17272/81‑1 (MQ=255)                                                                      taCCTGCTGGTTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGaa       >  1:17295/1‑81 (MQ=255)                                                                        ccTGCTGGTTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAAc      <  1:17273/80‑1 (MQ=255)                                                                        ccTGCTGGTTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACg     >  1:17304/1‑81 (MQ=255)                                                                        ccTGCTGGTTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACg     >  2:17302/1‑81 (MQ=255)                                                                           gctgGTTTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGgtg  >  2:17293/1‑81 (MQ=255)                                                                                |                                                                            TTCTGAAAGTTGACTTGCCTGCATCATGTGTGACTGAGTATTGGTGTAAAATCACCCGCCAGCAGATTATACCTGCTGG‑TTTTTTTTATTCTCGCCGCGCTAAAAAGGGAACGTATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTG  >  NC_000913/77507‑77660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑