| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,333,636 | 0 | A | . | 38.1% | 42.0 / 24.2 | 21 | coding (2589/2598 nt) | topA | DNA topoisomerase 1 |
| Reads supporting (aligned to +/- strand): ref base A (2/11); new base . (2/6); total (4/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTT > NC_000913/1333556‑1333700 | cAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGa < 1:233374/81‑1 (MQ=255) aGCAATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGaa < 1:233376/81‑1 (MQ=255) aGCAATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGaa < 2:233375/81‑1 (MQ=255) ttCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGG‑AAAAAATAACCtt < 1:233377/79‑1 (MQ=255) cGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCttt < 1:233378/79‑1 (MQ=255) cGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCttt < 1:233379/79‑1 (MQ=255) cGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCttt < 1:233380/79‑1 (MQ=255) aaGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGG‑AAAAAATAACCTTTAATTCTGt > 2:233403/1‑80 (MQ=255) aaGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGtttt < 2:233381/81‑1 (MQ=255) ggCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTAAAGG‑AAAAAATAACCTTTAATTCTGTCAGGTTTTtat < 1:233382/81‑1 (MQ=255) gACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGG‑AAAAAATAACCTTTAATTCTGTCAGGTTTTTATaaa > 1:233405/1‑81 (MQ=255) ggTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCCTTAATTCTGTCAGGTTTTTATAAACAAAggg > 2:233406/1‑81 (MQ=255) gTCGGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAggg < 2:233384/80‑1 (MQ=255) gTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGt < 1:233383/81‑1 (MQ=255) aTGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCg < 2:233385/80‑1 (MQ=255) tGTTGATGGCAAATGGGTTGAAGG‑AAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGcc < 2:233386/81‑1 (MQ=255) tGTTGATGGCAAATGGGTTGAAGG‑AAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGc < 1:233387/80‑1 (MQ=255) aTGGCAAATGGGTTGAAGG‑AAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCttt < 1:233388/80‑1 (MQ=255) tGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCtttt < 2:233389/81‑1 (MQ=255) tGGCAAATGGGTTGAAGG‑AAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCttttt < 1:233390/81‑1 (MQ=255) ggCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCttttt > 1:233402/1‑81 (MQ=255) gCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCtttttt < 2:233392/81‑1 (MQ=255) gCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCttttt < 2:233391/80‑1 (MQ=255) gCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCttttt < 2:233393/80‑1 (MQ=255) | CAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCATTTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAACCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTT > NC_000913/1333556‑1333700 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |