Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	77,704	C→T	A28A (GCC→GCT)	setA →	sugar exporter SetA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	77,704	0	C	T	100.0%	114.6 / NA	33	A28A (GCC→GCT)	setA	sugar exporter SetA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/13);  total (20/13)

CTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTACATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTGGATCGGCCTC  >  NC_000913/77626‑77779                                                                               |                                                                            cTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCTgg                                                                           >  1:17300/1‑81 (MQ=255)    gATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCTgg                                                                           <  1:17279/78‑1 (MQ=255)    gATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCTgg                                                                           <  1:17278/78‑1 (MQ=255)             gATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCTGGGGCGCTACAGGc                                                               <  1:17280/81‑1 (MQ=255)              aTGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCTGGGGCGCTACAGGCt                                                              >  1:17308/1‑81 (MQ=255)               tGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCTGGGGCGCTACAGGCTc                                                             <  1:17281/81‑1 (MQ=255)               tGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCTGGGGCGCTACAGGCTc                                                             >  1:17326/1‑81 (MQ=255)                 gCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCTGGGGCGCTACAGGCTcc                                                            <  1:17282/80‑1 (MQ=255)                 gCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCTGGGGCGCTACAGGCTcc                                                            >  2:17320/1‑80 (MQ=255)                 gCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCTGGGGCGCTACAGGCTcc                                                            >  1:17313/1‑80 (MQ=255)                      ccGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCTGGGGCGCTACAGGCTCCTACAtt                                                      >  1:17323/1‑81 (MQ=255)                       cGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCTGGGGCGCTACAGGCTCCTACAtt                                                      >  2:17311/1‑80 (MQ=255)                         tATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCTGGGGCGCTACAGGCTCCTACATTGAg                                                   <  2:17283/81‑1 (MQ=255)                           tGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCTGGGGCGCTACAGGCTCCTACATTGAGc                                                  <  1:17284/80‑1 (MQ=255)                            gAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCTGGGGCGCTACAGGCTCCTACATTGAGCt                                                 >  2:17330/1‑80 (MQ=255)                              aCGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCTGGGGCGCTACAGGCTCCTACATTGAGCTTAt                                              >  2:17315/1‑81 (MQ=255)                                ggTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCTGGGGCGCTACAGGCTCCTACATTGAGCTTAttt                                            <  2:17285/81‑1 (MQ=255)                                  tgtTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCTGGGGCGCTACAGGCTCCTACATTGAGCTTATTTCt                                          >  2:17318/1‑81 (MQ=255)                                   gtTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCTGGGGCGCTACAGGCTCCTACATTGAGCTTATTTCt                                          >  2:17309/1‑80 (MQ=255)                                    tttACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCTGGGGCGCTACAGGCTCCTACATTGAGCTTATTTCt                                          >  2:17334/1‑79 (MQ=255)                                      tACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCTGGGGCGCTACAGGCTCCTACATTGAGCTTATTTCTGAGt                                      >  2:17325/1‑81 (MQ=255)                                       aCGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCTGGGGCGCTACAGGCTCCTACATTGAGCTTATTTCTGAGTc                                     >  2:17322/1‑81 (MQ=255)                                        cgcgGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCTGGGGCGCTACAGGCTCCTACATTGAGCTTATTTCTGAGTc                                     <  1:17286/80‑1 (MQ=255)                                            gCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCTGGGGCGCTACAGGCTCCTACATTGAGCTTATTTCTGAGTCGTGAg                                >  2:17327/1‑81 (MQ=255)                                               tttATGCTGGTCGCTTTTATGATGGGGGTGGCTGGGGCGCTACAGGCTCCTACATTGAGCTTATTTCTGAGTCGTGAGGtt                             >  1:17316/1‑81 (MQ=255)                                                  aTGCTGGTCGCTTTTATGATGGGGGTGGCTGGGGCGCTACAGGCTCCTACATTGAGCTTATTTCTGAGTCGTGAGGTTGgc                          >  2:17329/1‑81 (MQ=255)                                                       ggTCGCTTTTATGATGGGGGTGGCTGGGGCGCTACAGGCTCCTACATTGAGCTTATTTCTGAGTCGTGAGGTTGgcgcgc                      >  2:17317/1‑80 (MQ=255)                                                       ggTCGCTTTTATGATGGGGGTGGCTGGGGCGCTACAGGCTCCTACATTGAGCTTATTTCTGAGTCGTGAGGTTGgcgcgc                      >  2:17324/1‑80 (MQ=255)                                                        gTCGCTTTTATGATGGGGGTGGCTGGGGCGCTACAGGCTCCTACATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCa                     <  1:17287/80‑1 (MQ=255)                                                           gCTTTTATGATGGGGGTGGCTGGGGCGCTACAGGCTCCTACATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCt                 >  1:17335/1‑81 (MQ=255)                                                               ttATGATGGGGGTGGCTGGGGCGCTACAGGCTCCTACATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCttt               <  2:17288/79‑1 (MQ=255)                                                                     tgGGGGTGGCTGGGGCGCTACAGGCTCCTACATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTGGATCg       <  2:17289/81‑1 (MQ=255)                                                                          gTGGCTGGGGCGCTACAGGCTCCTACATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTGGATCGGCctc  <  1:17290/81‑1 (MQ=255)                                                                               |                                                                            CTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTGGTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTACATTGAGCTTATTTCTGAGTCGTGAGGTTGGCGCGCAACCTTTCTGGATCGGCCTC  >  NC_000913/77626‑77779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑