Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	698,541	G→T	R213S (CGT→AGT)	asnB ←	asparagine synthetase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	698,541	0	G	T	100.0%	110.5 / NA	31	R213S (CGT→AGT)	asnB	asparagine synthetase B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (10/21);  total (10/21)

AGACCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGAT  >  NC_000913/698465‑698616                                                                             |                                                                            aGACCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGc                                                                         >  2:135866/1‑81 (MQ=255)   accaccAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTc                                                                       <  1:135826/81‑1 (MQ=255)     caccaGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGt                                                                     <  2:135827/81‑1 (MQ=255)     caccaGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGt                                                                     >  1:135859/1‑81 (MQ=255)     caccaGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGt                                                                     >  2:135869/1‑81 (MQ=255)      accaGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGtt                                                                    <  1:135828/81‑1 (MQ=255)      accaGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGtt                                                                    <  2:135830/81‑1 (MQ=255)      accaGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGtt                                                                    <  2:135829/81‑1 (MQ=255)         aGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGttttt                                                                 <  1:135831/81‑1 (MQ=255)               cagcaCACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGt                                                           <  2:135833/81‑1 (MQ=255)               cagcaCACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGt                                                           <  1:135832/81‑1 (MQ=255)                agcaCACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGt                                                           >  2:135863/1‑80 (MQ=255)                   acacCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACg                                                       >  1:135867/1‑81 (MQ=255)                     acCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGtt                                                     <  2:135834/81‑1 (MQ=255)                           aGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCttt                                               <  1:135835/81‑1 (MQ=255)                              cacaTCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCAc                                            <  1:135836/81‑1 (MQ=255)                               acatcagacatcagATGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCAcc                                           <  1:135837/81‑1 (MQ=255)                                 atcagacatcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCg                                          >  2:135868/1‑80 (MQ=255)                                   cagacatcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCAt                                       >  2:135871/1‑81 (MQ=255)                                    agtcatcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATc                                      <  2:135838/78‑1 (MQ=255)                                     gacatcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCg                                     >  2:135865/1‑81 (MQ=255)                                         tcagaTGGCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGt                                 <  2:135839/81‑1 (MQ=255)                                            gaTGGCTTTTAACTGAATTTTCTAGTGCCTGAGTAAGCGCGTTTTTGTCGGTAAGGTTATCTTTAACCGCATCGTAGCCGa                              <  2:135840/81‑1 (MQ=255)                                                gCTTTTAACTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAAcc                           <  1:135841/80‑1 (MQ=255)                                                       aCTGAATCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCga                   >  1:135878/1‑81 (MQ=255)                                                           aaTCTTCCAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCgatg                 <  1:135842/79‑1 (MQ=255)                                                                 ccAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTaa          <  2:135844/80‑1 (MQ=255)                                                                 ccAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTaa          <  1:135843/80‑1 (MQ=255)                                                                  cAGTGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAg         <  1:135845/80‑1 (MQ=255)                                                                     tGCCTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACg     <  1:135846/81‑1 (MQ=255)                                                                        cTGACTCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGAt  >  1:135874/1‑81 (MQ=255)                                                                             |                                                                            AGACCACCAGAAAGCAGCACACCGTAAGGCACATCAGACATCAGATGGCTTTTAACTGAATCTTCCAGTGCCTGACGCAGCTCGTTTTTGTCGGTCACGTTATCTTTCACCGCATCGTAGTCGAACCAGTCGCGATGATAGTAAGAACGGAT  >  NC_000913/698465‑698616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑