Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	751,308	C→T	V288I (GTC→ATC)	ybgQ ←	putative fimbrial usher protein YbgQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	751,308	0	C	T	100.0%	84.4 / NA	24	V288I (GTC→ATC)	ybgQ	putative fimbrial usher protein YbgQ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/13);  total (11/13)

CATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGC  >  NC_000913/751230‑751384                                                                               |                                                                             cATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGAtgc                                                                            <  2:144787/81‑1 (MQ=255)         gTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGATGCGGCCTGAt                                                                    >  2:144825/1‑81 (MQ=255)            cccTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGATGCGGCCTGATTg                                                                  <  2:144788/80‑1 (MQ=255)             ccTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGATGCGGCCTGATTGAc                                                                <  1:144790/81‑1 (MQ=255)             ccTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGATGCGGCCTGATTGAc                                                                <  2:144789/81‑1 (MQ=255)                gAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGATGCGGCCTGATTGACTGa                                                             >  1:144827/1‑81 (MQ=255)                 aaCAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGATGCGGCCTGATTGACTg                                                              <  2:144791/79‑1 (MQ=255)                  aCAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGATGCGGCCTGATTGACTGATc                                                           <  1:144792/81‑1 (MQ=255)                     gACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGATGCGGCCTGATTGACTGATCGTc                                                        <  2:144793/81‑1 (MQ=255)                          aTTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGATGCGGCCTGATTGACTGATCGTCAcc                                                     <  2:144794/79‑1 (MQ=255)                             gAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGATGCGGCCTGATTGACTGATCGTCACCGTgg                                                 >  1:144824/1‑80 (MQ=255)                                  catcaATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGATGCGGCCTGATTGACTGATCGTCACCGTGGCATTgg                                           >  2:144823/1‑81 (MQ=255)                                      aataatAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGATGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCtg                                       >  1:144831/1‑81 (MQ=255)                                      aataatAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGATGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCtg                                       >  2:144821/1‑81 (MQ=255)                                      aataatAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGATGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCt                                        <  1:144795/80‑1 (MQ=255)                                        taataaACGGGCCTGGCGGGACTTTTTTCTGGTAAATGATGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCtgtg                                     <  2:144796/81‑1 (MQ=255)                                          ataaACGGGCCTGGCGGGACTTTTTTCTGGTAAATGATGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGc                                    <  1:144797/80‑1 (MQ=255)                                          ataaACGGGCCTGGCGGGACTTTTTTCTGGTAAATGATGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGc                                    <  2:144798/80‑1 (MQ=255)                                            aaaCGGGCCTGGCGGGACTTTTTTCTGGTAAATGATGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCaa                                  >  2:144852/1‑80 (MQ=255)                                                     tGGCGGGACTTTTTTCTGGTAAATGATGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAAt                        >  2:144826/1‑81 (MQ=255)                                                      ggCGGGACTTTTTTCTGGTAAATGATGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAAtt                       >  2:144832/1‑81 (MQ=255)                                                                      ggTAAATGATGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCAc       >  2:144830/1‑81 (MQ=255)                                                                       gTAAATGATGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCa        <  2:144799/79‑1 (MQ=255)                                                                        tAAATGATGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGc     <  2:144800/81‑1 (MQ=255)                                                                         aaaTGATGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACg      >  1:144835/1‑79 (MQ=255)                                                                           atgatgCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGc  <  2:144801/81‑1 (MQ=255)                                                                               |                                                                             CATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGC  >  NC_000913/751230‑751384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑