Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	753,415	A→G	Y352H (TAC→CAC)	gltA ←	citrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	753,415	0	A	G	100.0%	100.7 / NA	29	Y352H (TAC→CAC)	gltA	citrate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/11);  total (18/11)

AAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGCGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCA  >  NC_000913/753337‑753492                                                                               |                                                                              aaGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTGCg                                                                             >  1:145093/1‑81 (MQ=255)               aTCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTGCGGGTCGTTCAgcgc                                                                >  1:145100/1‑80 (MQ=255)                  gCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTGCGGGTCGTTCAGCGCGATg                                                            <  2:145067/81‑1 (MQ=255)                    tttCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTGCGGGTCGTTCAGCGCGATGtt                                                          >  2:145089/1‑81 (MQ=255)                       cAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTGCGGGTCGTTCAGCGCGATGtttt                                                        <  2:145068/80‑1 (MQ=255)                        aGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTGCGGGTCGTTCAGCGCGATGTTTTc                                                       >  1:145105/1‑80 (MQ=255)                        aGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTGCGGGTCGTTCAGCGCGATGTTTTc                                                       <  1:145069/80‑1 (MQ=255)                          gatgatACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTGCGGGTCGTTCAGCGCGATGTTTTCCAg                                                    >  2:145098/1‑81 (MQ=255)                             gatACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTGCGGGTCGTTCAGCGCGATGTTTTCCAGCTc                                                 >  2:145099/1‑81 (MQ=255)                                    gagTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTGCGGGTCGTTCAGCGCGATGTTTTCCAGCTCCATAGc                                           >  2:145127/1‑80 (MQ=255)                                          aaaTCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTGCGGGTCGTTCAGCGCGATGTTTTCCAGCTCCATAGCCACTTc                                     >  1:145094/1‑80 (MQ=255)                                          aaaTCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTGCGGGTCGTTCAGCGCGATGTTTTCCAGCTCCATAGCCACTTc                                     >  1:145107/1‑80 (MQ=255)                                                 cGTTCGGGTACAGTTTCTTCTCGATAAAGTGCGGGTCGTTCAGCGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGt                             >  2:145132/1‑81 (MQ=255)                                                 cGTTCGGGTACAGTTTCTTCTCGATAAAGTGCGGGTCGTTCAGCGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGt                             >  2:145112/1‑81 (MQ=255)                                                       ggTACAGTTTCTTCTCGATAAAGTGCGGGTCGTTCAGCGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCt                       <  2:145071/81‑1 (MQ=255)                                                       ggTACAGTTTCTTCTCGATAAAGTGCGGGTCGTTCAGCGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCt                       <  2:145070/81‑1 (MQ=255)                                                        gTACAGTTTCTTCTCGATAAAGTGCGGGTCGTTCAGCGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCtt                      <  2:145072/81‑1 (MQ=255)                                                            aGTTTCTTCTCGATAAAGTGCGGGTCGTTCAGCGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTg                  >  1:145122/1‑81 (MQ=255)                                                                tcttctCGATAAAGTGCGGGTCGTTCAGCGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGcc                >  1:145130/1‑79 (MQ=255)                                                                 cttctCGATAAAGTGCGGGTCGTTCAGCGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCa              <  2:145073/80‑1 (MQ=255)                                                                 cttctCGATAAAGTGCGGGTCGTTCAGCGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAg             >  1:145101/1‑81 (MQ=255)                                                                    ctcGATAAAGTGCGGGTCGTTCAGCGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGct           <  1:145074/80‑1 (MQ=255)                                                                    ctcGATAAAGTGCGGGTCGTTCAGCGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGc            <  1:145075/79‑1 (MQ=255)                                                                       gATAAAGTGCGGGTCGTTCAGCGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCttt       >  1:145131/1‑81 (MQ=255)                                                                       gATAAAGTGCGGGTCGTTCAGCGCGATGTTTTCCAGCTCCATAGCCACTCCCAGCAGGCCATCCTTCGTGCCCAGCTCttt       >  1:145119/1‑81 (MQ=255)                                                                        aTAAAGTGCGGGTCGTTCAGCGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCttt       >  2:145123/1‑80 (MQ=255)                                                                        aTAAAGTGCGGGTCGTTCAGCGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTc      <  1:145076/81‑1 (MQ=255)                                                                           aaGTGCGGGTCGTTCAGCGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGc   <  1:145077/81‑1 (MQ=255)                                                                            aGTGCGGGTCGTTCAGCGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCa  >  1:145120/1‑81 (MQ=255)                                                                               |                                                                              AAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTACAGTTTCTTCTCGATAAAGTACGGGTCGTTCAGCGCGATGTTTTCCAGCTCCATAGCCACTTCCAGCAGGTCATCCTTCGTGCCCAGCTCTTTCAGCA  >  NC_000913/753337‑753492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑