Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	797,736	T→C	E232E (GAA→GAG)	ybhA ←	pyridoxal phosphate/fructose‑1,6‑bisphosphate phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	797,736	0	T	C	100.0%	83.0 / NA	24	E232E (GAA→GAG)	ybhA	pyridoxal phosphate/fructose‑1,6‑bisphosphate phosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/18);  total (6/18)

TTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCA  >  NC_000913/797659‑797813                                                                              |                                                                              ttATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTcc                                                                            <  1:152816/81‑1 (MQ=255)   aTCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCa                                                                           <  1:152817/80‑1 (MQ=255)    tCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAg                                                                          <  1:152818/80‑1 (MQ=255)         aaTCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACt                                                                    >  1:152872/1‑81 (MQ=255)              caATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGatat                                                               >  2:152861/1‑81 (MQ=255)                       cgcgcgcTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGt                                                      <  2:152819/81‑1 (MQ=255)                       cgcgcgcTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGt                                                      >  1:152869/1‑81 (MQ=255)                        gcgcgcTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGtt                                                     <  2:152820/81‑1 (MQ=255)                         cgcgcTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTa                                                    <  2:152821/81‑1 (MQ=255)                            gcTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCg                                                 >  1:152871/1‑81 (MQ=255)                                ttACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGcc                                               <  1:152822/79‑1 (MQ=255)                                   ccGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAAt                                           <  2:152823/80‑1 (MQ=255)                                   ccGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAAt                                           <  1:152824/80‑1 (MQ=255)                                     gcgTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGcc                                        <  2:152825/81‑1 (MQ=255)                                     gcgTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGc                                         >  1:152874/1‑80 (MQ=255)                                             ggCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGtt                                <  1:152827/81‑1 (MQ=255)                                             ggCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGt                                 <  2:152826/80‑1 (MQ=255)                                              gCGTTGCCCATCGCCACGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGttt                               <  1:152828/81‑1 (MQ=255)                                                    cccATCGCCACGCCTGTACCAGCGGCCTCCGGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCAt                          <  2:152829/80‑1 (MQ=255)                                                             aCGCCTGTACCAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCt                >  1:152870/1‑81 (MQ=255)                                                                      ccAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTc        <  2:152831/80‑1 (MQ=255)                                                                      ccAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTc        <  2:152832/80‑1 (MQ=255)                                                                      ccAGCGGCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTc        <  1:152830/80‑1 (MQ=255)                                                                           ggCCTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCa  <  1:152833/81‑1 (MQ=255)                                                                              |                                                                              TTATCACCAATCACAATGTTGGCGCGCGCTTTTACCGCGTCATCGGCGTTGCCCATCGCCACGCCTGTACCAGCGGCTTCCAGCATACTGATATCATTAAAGTTATCGCCGAATGCCACGACGTTTTCCATCGACCAACCTTGCGCCTCAACCCA  >  NC_000913/797659‑797813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑