Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	808,026	T→C	Q411R (CAG→CGG)	bioA ←	adenosylmethionine‑8‑amino‑7‑oxononanoate aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	808,026	0	T	C	100.0%	69.7 / NA	21	Q411R (CAG→CGG)	bioA	adenosylmethionine‑8‑amino‑7‑oxononanoate aminotransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/8);  total (13/8)

CCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGTTTGCCAAAAGGCCGGATCCAGACACCC  >  NC_000913/807950‑808100                                                                             |                                                                           ccTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCCGCaa                                                                        <  2:154512/81‑1 (MQ=255)         ggACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCCGCAACTGTTGCg                                                                >  1:154542/1‑81 (MQ=255)            cTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCCGCAACTGTTGCGGga                                                             <  1:154513/81‑1 (MQ=255)               ctcGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCCGCAACTGTTGCGGgaga                                                           <  2:154514/80‑1 (MQ=255)                  gTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCCGCAACTGTTGCGGGAGaataa                                                       <  1:154515/81‑1 (MQ=255)                          cAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCCGCAACTGTTGCGGGAGAATAATATAGggc                                               <  2:154516/81‑1 (MQ=255)                             aaaaaTGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCCGCAACTGTTGCGGGAGAATAATATAGggcgg                                             >  1:154535/1‑80 (MQ=255)                                   gTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCCGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAgg                                      >  1:154532/1‑81 (MQ=255)                                        aTCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCCGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAAt                                 >  2:154537/1‑81 (MQ=255)                                           cTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCCGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAg                              >  1:154538/1‑81 (MQ=255)                                            tGTACCGCGCGGTTAACCGCTGCGGTCAGACGCCGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGt                             >  2:154536/1‑81 (MQ=255)                                              tACCGCGCGGTTAACCGCTGCGGTCAGACGCCGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGttt                           >  2:154534/1‑81 (MQ=255)                                              tACCGCGCGGTTAACCGCTGCGGTCAGACGCCGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGtt                            <  2:154517/80‑1 (MQ=255)                                                  gcgcgGTTAACCGCTGCGGTCAGACGCCGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGTTTGcc                        >  1:154539/1‑80 (MQ=255)                                                      ggTTAACCGCTGCGGTCAGACGCCGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGTTTGCCAAAAg                   <  1:154518/81‑1 (MQ=255)                                                             cGCTGCGGTCAGACGCCGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGTTTGCCAAAAGGCCGGAt            >  2:154545/1‑81 (MQ=255)                                                                   ggTCAGACGCCGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGTTTGCCAAAAGGCCGGATCCAGa       >  1:154541/1‑80 (MQ=255)                                                                   ggTCAGACGCCGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGTTTGCCAAAAGGCCGGATCCAGa       >  2:154544/1‑80 (MQ=255)                                                                   ggTCAGACGCCGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGTTTGCCAAAAGGCCGGATCCAGa       >  2:154550/1‑80 (MQ=255)                                                                       aGACGCCGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGTTTGCCAAAAGGCCGGATCCAGACAccc  >  1:154547/1‑81 (MQ=255)                                                                       aGACGCCGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGTTTGCCAAAAGGCCGGATCCAGACAccc  <  2:154519/81‑1 (MQ=255)                                                                             |                                                                           CCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGCGCGGTTAACCGCTGCGGTCAGACGCTGCAACTGTTGCGGGAGAATAATATAGGGCGGCATCAGGTAAATCAGTTTGCCAAAAGGCCGGATCCAGACACCC  >  NC_000913/807950‑808100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑