Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	79,341	C→A	G38V (GGT→GTT)	leuD ←	3‑isopropylmalate dehydratase subunit LeuD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	79,341	0	C	A	100.0%	123.6 / NA	36	G38V (GGT→GTT)	leuD	3‑isopropylmalate dehydratase subunit LeuD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (11/25);  total (11/25)

TTCAGCACGAAGTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAACCCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTGGCGGCATCCAGCGGAACC  >  NC_000913/79263‑79418                                                                               |                                                                              ttCAGCACGAAGTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAacc                                                                             >  2:17613/1‑81 (MQ=255) ttCAGCACGAAGTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAacc                                                                             <  2:17565/81‑1 (MQ=255)   cAGCACGAAGTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCg                                                                            <  2:17566/80‑1 (MQ=255)    aGCACGAAGTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTa                                                                          <  1:17568/81‑1 (MQ=255)    aGCACGAAGTCCAGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACGGATGCGCGCCAAAAACCGt                                                                           <  2:17567/80‑1 (MQ=255)           aGTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCa                                                                   >  2:17623/1‑81 (MQ=255)               cGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACttt                                                               >  1:17627/1‑81 (MQ=255)               cGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACtt                                                                <  1:17569/80‑1 (MQ=255)               cGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACtt                                                                <  1:17570/80‑1 (MQ=255)                gggTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTc                                                              >  2:17612/1‑81 (MQ=255)                gggTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTc                                                              <  2:17572/81‑1 (MQ=255)                gggTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTc                                                              <  1:17571/81‑1 (MQ=255)                    ttGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGc                                                           >  2:17614/1‑80 (MQ=255)                     tGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTGCTGCa                                                          <  2:17573/80‑1 (MQ=255)                             ggCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGt                                                  <  2:17574/80‑1 (MQ=255)                                cTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCg                                              >  1:17629/1‑81 (MQ=255)                                cTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCg                                              <  2:17575/81‑1 (MQ=255)                                 tttttCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCgg                                             >  2:17620/1‑81 (MQ=255)                                   tttCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAACCTGTTTCgg                                             <  2:17576/79‑1 (MQ=255)                                       aTCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGgatgat                                       <  1:17577/81‑1 (MQ=255)                                       aTCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGgatgat                                       <  2:17578/81‑1 (MQ=255)                                        tCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGAtt                                      <  1:17580/81‑1 (MQ=255)                                        tCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGAtt                                      <  2:17579/81‑1 (MQ=255)                                          cAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTg                                     <  2:17581/80‑1 (MQ=255)                                               aaCGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCgg                               >  1:17622/1‑81 (MQ=255)                                                aCGCCAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCgg                               >  1:17630/1‑80 (MQ=255)                                                   ccAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATc                           <  1:17582/81‑1 (MQ=255)                                                    cAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATc                           >  1:17624/1‑80 (MQ=255)                                                    cAGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCg                          <  1:17583/81‑1 (MQ=255)                                                     aGTCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGa                         <  2:17584/81‑1 (MQ=255)                                                       tCGTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACa                       <  1:17585/81‑1 (MQ=255)                                                         gTTAAACAGATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACAtt                     >  1:17618/1‑81 (MQ=255)                                                             aaCAGATGCGCGCCAAAAACCGGACGGGGCACGGGCGGCAAAAACGGGGGCGGGATGATTGCATCGGTATCGACATTggcg                 <  1:17586/81‑1 (MQ=255)                                                                 gATGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTGGCGGCAt             >  2:17619/1‑81 (MQ=255)                                                                   tGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTGGCGGCATcc           <  2:17588/81‑1 (MQ=255)                                                                   tGCGCGCCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTGGCGGCATcc           <  1:17587/81‑1 (MQ=255)                                                                     cgcgcCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTGGCGGCATCCAg         <  1:17589/81‑1 (MQ=255)                                                                      gcgcCAAAAACCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTGGCGGCATCCAGc        <  2:17590/81‑1 (MQ=255)                                                                            aaaaCCGTACGGGTCACTTTCTGCAAAAACTATTTCGGGATGATTGCATCGGTATCGACATTGGCGGCATCCAGCGGAacc  <  2:17591/81‑1 (MQ=255)                                                                               |                                                                              TTCAGCACGAAGTCCGGGTTTGGCTGTTGGCCTTTTTCATCCAGAAAACGCCAGTCGTTAAACAGATGCGCGCCAAAACCCGTACGGGTCACTTTCTGCAAAAACTGTTTCGGGATGATTGCATCGGTATCGACATTGGCGGCATCCAGCGGAACC  >  NC_000913/79263‑79418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑