Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	814,361	A→G	K279R (AAA→AGA)	uvrB →	excision nuclease subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	814,361	0	A	G	100.0%	113.9 / NA	33	K279R (AAA→AGA)	uvrB	excision nuclease subunit B
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/20);  total (13/20)

CGAGCGCATCGTACAGGCGATGGAGGAGATCAAAGAAGAGCTGGCCGCCAGACGCAAAGTGCTGTTGGAAAACAACAAACTGCTGGAAGAGCAGCGGCTGACCCAGCGTACCCAGTTTGATCTGGAGATGATGAACGAGCTGGGCTACTGTTC  >  NC_000913/814284‑814436                                                                              |                                                                            cgAGCGCATCGTACAGGCGATGGAGGAGATCAAAGAAGAGCTGGCCGCCAGACGCAAAGTGCTGTTGGAAAACAACAGAct                                                                          >  1:155655/1‑81 (MQ=255)       cATCGTACAGGCGATGGAGGAGATCAAAGAAGAGCTGGCCGCCAGACGCAAAGTGCTGTTGGAAAACAACAGACTGCTgg                                                                     >  2:155661/1‑80 (MQ=255)        aTCGTACAGGCGATGGAGGAGATCAAAGAAGAGCTGGCCGCCAGACGCAAAGTGCTGTTGGAAAACAACAGACTGCTGGaa                                                                   <  1:155620/81‑1 (MQ=255)           gTACAGGCGATGGAGGAGATCAAAGAAGAGCTGGCCGCCAGACGCAAAGTGCTGTTGGAAAACAACAGACTGCTGGAaga                                                                 <  1:155621/80‑1 (MQ=255)             aCAGGCGATGGAGGAGATCAAAGAAGAGCTGGCCGCCAGACGCAAAGTGCTGTTGGAAAACAACAGATTGCTGGAagag                                                                >  1:155662/1‑79 (MQ=255)             aCAGGCGATGGAGGAGATCAAAGAAGAGCTGGCCGCCAGACGCAAAGTGCTGTTGGAAAACAACAGACTGCTGGAagag                                                                >  1:155670/1‑79 (MQ=255)               aGGCGATGGAGGAGATCAAAGAAGAGCTGGCCGCCAGACGCAAAGTGCTGTTGGAAAACAACAGACTGCTGGAAGagcagc                                                            <  2:155622/81‑1 (MQ=255)                ggCGATGGAGGAGATCAAAGAAGAGCTGGCCGCCAGACGCAAAGTGCTGTTGGAAAACAACAGACTGCTGGAAGAGCAGCg                                                           <  2:155623/81‑1 (MQ=255)                 gCGATGGAGGAGATCAAAGAAGAGCTGGCCGCCAGACGCAAAGTGCTGTTGGAAAACAACAGACTGCTGGAAGAGCAGCgg                                                          <  2:155625/81‑1 (MQ=255)                 gCGATGGAGGAGATCAAAGAAGAGCTGGCCGCCAGACGCAAAGTGCTGTTGGAAAACAACAGACTGCTGGAAGAGCAGCgg                                                          <  1:155624/81‑1 (MQ=255)                   gATGGAGGAGATCAAAGAAGAGCTGGCCGCCAGACGCAAAGTGCTGTTGGAAAACAACAGACTGCTGGAAGAGCAGCGGCt                                                        >  2:155656/1‑81 (MQ=255)                      ggaggaGATCAAAGAAGAGCTGGCCGCCAGACGCAAAGTGCTGTTGGAAAACAACAGACTGCTGGAAGAGCAGCGGCTGAc                                                     >  2:155659/1‑81 (MQ=255)                            gaTCAAAGAAGAGCTGGCCGCCAGACGCAAAGTGCTGTTGGAAAACAACTGACTGCTGGAAGAGCAGCGGCTGACCCAGCg                                               >  2:155673/1‑81 (MQ=255)                             aTCAAAGAAGAGCTGGCCGCCAGACGCAAAGTGCTGTTGGAAAACAACAGACTGCTGGAAGAGCAGCGGCTGACCCAGCGt                                              <  1:155626/81‑1 (MQ=255)                                aaaGAAGAGCTGGCCGCCAGACGCAAAGTGCTGTTGGAAAACAACAGACTGCTGGAAGAGCAGCGGCTGACCCAGCGTAc                                            <  2:155627/80‑1 (MQ=255)                                     agagCTGGCCGCCAGACGCAAAGTGCTGTTGGAAAACAACAGACTGCTGGAAGAGCAGCGGCTGACCCAGCGTACCCAGtt                                      <  2:155628/81‑1 (MQ=255)                                      gagCTGGCCGCCAGACGCAAAGTGCTGTTGGAAAACAACAGACTGCTGGAAGAGCAGCGGCTGACCCAGCGTACCCAGttt                                     <  1:155630/81‑1 (MQ=255)                                      gagCTGGCCGCCAGACGCAAAGTGCTGTTGGAAAACAACAGACTGCTGGAAGAGCAGCGGCTGACCCAGCGTACCCAGttt                                     <  1:155629/81‑1 (MQ=255)                                        gCTGGCCGCCAGACGCAAAGTGCTGTTGGAAAACAACAGACTGCTGGAAGAGCAGCGGCTGACCCAGCGTACCCAGTTTGa                                   <  1:155631/81‑1 (MQ=255)                                            gccgccAGACGCAAAGTGCTGTTGGAAAACAACAGACTGCTGGAAGAGCAGCGGCTGACCCAGCGTACCCAGTTTGATCt                                <  2:155632/80‑1 (MQ=255)                                               gccAGACGCAAAGTGCTGTTGGAAAACAACAGACTGCTGGAAGAGCAGCGGCTGACCCAGCGTACCCAGTTTGATCTg                               >  1:155685/1‑78 (MQ=255)                                               gccAGACGCAAAGTGCTGTTGGAAAACAACAGACTGCTGGAAGAGCAGCGGCTGACCCAGCGTACCCAGTTTGATCTGgag                            <  2:155633/81‑1 (MQ=255)                                                  aGACGCAAAGTGCTGTTGGAAAACAACAGACTGCTGGAAGAGCAGCGGCTGACCCAGCGTACCCAGTTTGATCTGGAgatg                         <  2:155634/81‑1 (MQ=255)                                                      gCAAAGTGCTGTTGGAAAACAACAGACTGCTGGAAGAGCAGCGGCTGACCCAGCGTACCCAGTTTGATCTGGAGAtgatga                     <  1:155636/81‑1 (MQ=255)                                                      gCAAAGTGCTGTTGGAAAACAACAGACTGCTGGAAGAGCAGCGGCTGACCCAGCGTACCCAGTTTGATCTGGAGAtgatga                     <  1:155635/81‑1 (MQ=255)                                                           gTGCTGTTGGAAAACAACAGACTGCTGGAAGAGCAGCGGCTGACCCAGCGTACCCAGTTTGATCTGGAGATGATGAACGAg                >  1:155680/1‑81 (MQ=255)                                                              cTGTTGGAAAACAACAGACTGCTGGAAGAGCAGCGGCTGACCCAGCGTACCCAGTTTGATCTGGAGATGATGAACGAGCTg             >  1:155671/1‑81 (MQ=255)                                                                 ttGGAAAACAACAGACTGCTGGAAGAGCAGCGGCTGACCCAGCGTACCCAGTTTGATCTGGAGATGATGAACGAGCTGGGc          >  1:155677/1‑81 (MQ=255)                                                                 ttGGAAAACAACAGACTGCTGGAAGAGCAGCGGCTGACCCAGCGTACCCAGTTTGATCTGGAGATGATGAACGAGCTGGGc          <  1:155637/81‑1 (MQ=255)                                                                    gAAAACAACAGACTGCTGGAAGAGCAGCGGCTGACCCAGCGTACCCAGTTTGATCTGGAGATGATGAACGAGCTGGGCTAc       <  2:155638/81‑1 (MQ=255)                                                                     aaaaCAACAGACTGCTGGAAGAGCAGCGGCTGACCCAGCGTACCCAGTTTGATCTGGAGATGATGAACGAGCTGGGCTACt      <  1:155639/81‑1 (MQ=255)                                                                      aaaCAACAGACTGCTGGAAGAGCAGCGGCTGACCCAGCGTACCCAGTTTGATCTGGAGATGATGAACGAGCTGGGCTACTg     >  1:155675/1‑81 (MQ=255)                                                                      aaaCAACAGACTGCTGGAAGAGCAGCGGCTGACCCAGCGTACCCAGTTTGATCTGGAGATGATGAACGAGCTGGGCTACTg     >  1:155672/1‑81 (MQ=255)                                                                         caacagACTGCTGGAAGAGCAGCGGCTGACCCAGCGTACCCAGTTTGATCTGGAGATGATGAACGAGCTGGGCTACTGTTc  >  2:155678/1‑81 (MQ=255)                                                                              |                                                                            CGAGCGCATCGTACAGGCGATGGAGGAGATCAAAGAAGAGCTGGCCGCCAGACGCAAAGTGCTGTTGGAAAACAACAAACTGCTGGAAGAGCAGCGGCTGACCCAGCGTACCCAGTTTGATCTGGAGATGATGAACGAGCTGGGCTACTGTTC  >  NC_000913/814284‑814436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑