Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	881,661	T→G	L312R (CTC→CGC)	dacC →	D‑alanyl‑D‑alanine carboxypeptidase DacC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	881,661	0	T	G	100.0%	63.9 / NA	19	L312R (CTC→CGC)	dacC	D‑alanyl‑D‑alanine carboxypeptidase DacC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (13/6);  total (13/6)

GTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAGCGCGTCTGGTTTGGTGATAAGAGCGAAGTGAATCTCGGGGCAGGTGAAGCGGGCTCAGTGACCATACCGCGTGGGCAGCTGAAAAACCTGAAAGCGAGTTATAC  >  NC_000913/881588‑881730                                                                          |                                                                      gTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAGCGCGTCTGGTTTGGTGATAAGAGCGAAGTGAATCGCgggg                                                                  >  1:166996/1‑79 (MQ=255)             aaaCCTGATGCCACCTTTGTGACTCAGCGCGTCTGGTTTGGTGATAAGAGCGAAGTGAATCGCGGGGCAGGTGAAGCGGGc                                                    >  2:166966/1‑81 (MQ=255)              aaCCTGATGCCACCTTTGTGACTCAGCGCGTCTGGTTTGGTGATAAGAGCGAAGTGAATCGCGGGGCAGGTGAAGCGGGCt                                                   <  2:166938/81‑1 (MQ=255)                    aTGCCACCTTTGTGACTCAGCGCGTCTGGTTTGGTGATAAGAGCGAAGTGAATCGCGGGGCAGGTGAAGCGGGCTCAGTGa                                             >  2:166973/1‑81 (MQ=255)                     tGCCACCTTTGTGACTCAGCGCGTCTGGTTTGGTGATAAGAGCGAAGTGAATCGCGGGGCAGGTGAAGCGGGCTCAGTGAc                                            <  2:166939/81‑1 (MQ=255)                         aCCTTTGTGACTCAGCGCGTCTGGTTTGGTGATAAGAGCGAAGTGAATCGCGGGGCAGGTGAAGCGGGCTCAGTGACCATa                                        <  2:166940/81‑1 (MQ=255)                               gtgACTCAGCGCGTCTGGTTTGGTGATAAGAGCGAAGTGAATCGCGGGGCAGGTGAAGCGGGCTCAGTGACCATACCGCGt                                  >  1:166965/1‑81 (MQ=255)                               gtgACTCAGCGCGTCTGGTTTGGTGATAAGAGCGAAGTGAATCGCGGGGCAGGTGAAGCGGGCTCAGTGACCATACCGCGt                                  >  2:166968/1‑81 (MQ=255)                               gtgACTCAGCGCGTCTGGTTTGGTGATAAGAGCGAAGTGAATCGCGGGGCAGGTGAAGCGGGCTCAGTGACCATACCGCGt                                  >  1:166977/1‑81 (MQ=255)                                  aCTCAGCGCGTCTGGTTTGGTGATAAGAGCGAAGTGAATCGCGGGGCAGGTGAAGCGGGCTCAGTGACCATACCGCGTggg                               <  1:166941/81‑1 (MQ=255)                                    tCAGCGCGTCTGGTTTGGTGATAAGAGCGAAGTGAATCGCGGGGCAGGTGAAGCGGGCTCAGTGACCATACCGCGTggg                               >  1:166942/1‑79 (MQ=255)                                     cAGCGCGTCTGGTTTGGTGATAAGAGCGAAGTGAATCGCGGGGCAGGTGAAGCGGGCTCAGTGACCATACCGCGTGGGCAg                            >  1:166980/1‑81 (MQ=255)                                         gcgTCTGGTTTGGTGATAAGAGCGAAGTGAATCGCGGGGCAGGTGAAGCGGGCTCAGTGACCATACCGCGTGGGCAGCTg                         >  2:166972/1‑80 (MQ=255)                                           gTCTGGTTTGGTGATAAGAGCGAAGTGAATCGCGGGGCAGGTGAAGCGGGCTCAGTGACCATACCGCGTGGGCAGCTGaa                       >  2:166983/1‑80 (MQ=255)                                            tCTGGTTTGGTGATAAGAGCGAAGTGAATCGCGGGGCAGGTGAAGCGGGCTCAGTGACCATACCGCGTGGGCAGCTGaaaa                     <  1:166943/81‑1 (MQ=255)                                               ggTTTGGTGATAAGAGCGAAGTGAATCGCGGGGCAGGTGAAGCGGGCTCAGTGACCATACCGCGTGGGCAGCTGAAAAAcc                  >  1:166969/1‑81 (MQ=255)                                                    ggTGATAAGAGCGAAGTGAATCGCGGGGCAGGTGAAGCGGGCTCAGTGACCATACCGCGTGGGCAGCTGAAAAACCTGaa              <  2:166944/80‑1 (MQ=255)                                                      tGATAAGAGCGAAGTGAATCGCGGGGCAGGTGAAGCGGGCTCAGTGACCATACCGCGTGGGCAGCTGAAAAACCTGAAAGc           >  2:166974/1‑81 (MQ=255)                                                               cGAAGTGAATCGCGGGGCAGGTGAAGCGGGCTCAGTGACCATACCGCGTGGGCAGCTGAAAAACCTGAAAGCGAGTTATAc  >  1:166971/1‑81 (MQ=255)                                                                          |                                                                      GTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAGCGCGTCTGGTTTGGTGATAAGAGCGAAGTGAATCTCGGGGCAGGTGAAGCGGGCTCAGTGACCATACCGCGTGGGCAGCTGAAAAACCTGAAAGCGAGTTATAC  >  NC_000913/881588‑881730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑