Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	934,494	C→T	A424V (GCG→GTG)	ftsK →	cell division DNA translocase FtsK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	934,494	0	C	T	100.0%	100.7 / NA	29	A424V (GCG→GTG)	ftsK	cell division DNA translocase FtsK
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/17);  total (12/17)

AGTACAGCCGCAGCAGCCGTATTATGCACCTGCAGCTGAACAACCTGCGCAACAGCCGTATTATGCCCCTGCGCCAGAACAACCGGTGGCAGGTAACGCCTGGCAAGCCGAAGAGCAGCAATCCACTTTTGCTCCACAGTCTACATACC  >  NC_000913/934423‑934571                                                                        |                                                                              aGTACAGCCGCAGCAGCCGTATTATGCACCTGCAGCTGGACAACCTGCGCAACAGCCGTATTATGCCCCTGTGCCAGaaca                                                                      <  2:175503/81‑1 (MQ=255) aGTACAGCCGCAGCAGCCGTATTATGCACCTGCAGCTGAACAACCTGCGCAACAGCCGTATTATGCCCCTGTGCCAGaaca                                                                      <  1:175502/81‑1 (MQ=255)  gTACAGCCGCAGCAGCCGTATTATGCACCTGCAGCTGAACAACCTGCGCAACAGCCGTATTATGCCCCTGTGCCAGaacaa                                                                     >  2:175537/1‑81 (MQ=255)   tACAGCCGCAGCAGCCGTATTATGCACCTGCAGCTGAACAACCTGCGCAACAGCCGTATTATGCCCCTGTGCCAGaacaac                                                                    >  1:175540/1‑81 (MQ=255)          gcagcaGCCGTATTATGCACCTGCAGCTGAACAACCTGCGCAACAGCCGTATTATGCCCCTGTGCCAGAACAACCGGTgg                                                              >  2:175544/1‑80 (MQ=255)          gcagcaGCCGTATTATGCACCTGCAGCTGAACAACCTGCGCAACAGCCGTATTATGCCCCTGTGCCAGAACAACCGGTGGc                                                             <  1:175504/81‑1 (MQ=255)              cagcCGTATTATGCACCTGCAGCTGAACAACCTGCGCAACAGCCGTATTATGCCCCTGTGCCAGAACAACCGGTGGCAGGt                                                         <  1:175505/81‑1 (MQ=255)               agcCGTATTATGCACCTGCAGCTGAACAACCTGCGCAACAGCCGTATTATGCCCCTGTGCCAGAACAACCGGTGGCAAGTa                                                        <  2:175507/81‑1 (MQ=255)               agcCGTATTATGCACCTGCAGCTGAACAACCAGCGCAACAGCCGTATTATGCCCCTGTGCCAGAACAACCGGTGGCAGGTa                                                        <  2:175506/81‑1 (MQ=255)                  cGTATTATGCACCTGCAGCTGAACAACCTGCGCAACAGCCGTATTATGCCCCTGTGCCAGAACAACCGGTGGCAGGTAACg                                                     <  2:175508/81‑1 (MQ=255)                  cGTATTATGCACCTGCAGCTGAACAACCTGCGCAACAGCCGTATTATGCCCCTGTGCCAGAACAACCGGTGGCAGGTAACg                                                     >  1:175531/1‑81 (MQ=255)                  cGTATTATGCACCTGCAGCTGAACAACCTGCGCAACAGCCGTATTATGCCCCTGTGCCAGAACAACCGGTGGCAGGTAACg                                                     >  1:175536/1‑81 (MQ=255)                    tattatGCACCTGCAGCTGAACAACCTGCGCAACAGCCGTATTATGCCCCTGTGCCAGAACAACCGGTGGCAGGTAACGcc                                                   >  2:175543/1‑81 (MQ=255)                      ttatGCACCTGCAGCTGAACAACCTGCGCAACAGCCGTATTATGCCCCTGTGCCAGAACAACCGGTGGCAGGTAACGCCTg                                                 <  1:175509/81‑1 (MQ=255)                              cTGCAGCTGAACAACCTGCGCAACAGCCGTATTATGCCCCTGTGCCAGAACAACCGGTGGCAGGTAACGCCTGGCAAGcc                                          >  2:175534/1‑80 (MQ=255)                                 cAGCTGAACAACCTGCGCAACAGCCGTATTATGCCCCTGTGCCAGAACAACCGGTGGCAGGTAACGCCTGGCAAGCCg                                         >  2:175555/1‑78 (MQ=255)                                 cAGCTGAACAACCTGCGCAACAGCCGTATTATGCCCCTGTGCCAGAACAACCGGTGGCAGGTAACGCCTGGCAAGCCGaa                                       <  2:175510/80‑1 (MQ=255)                                 cAGCTGAACAACCTGCGCAACAGCCGTATTATGCCCCTGTGCCAGAACAACCGGTGGCAGGTAACGCCTGGCAAGCCGAag                                      <  1:175512/81‑1 (MQ=255)                                 cAGCTGAACAACCTGCGCAACAGCCGTATTATGCCCCTGTGCCAGAACAACCGGTGGCAGGTAACGCCTGGCAAGCCGAag                                      <  1:175511/81‑1 (MQ=255)                                      gAACAACCTGCGCAACAGCCGTATTATGCCCCTGTGCCAGAACAACCGGTGGCAGGTAACGCCTGGCAAGCCGAAGagcag                                 >  2:175538/1‑81 (MQ=255)                                            ccTGCGCAACAGCCGTATTATGCCCCTGTGCCAGAACAACCGGTGGCAGGTAACGCCTGGCAAGCCGAAGAGCAGCAATc                            >  1:175532/1‑80 (MQ=255)                                                 gcAACAGCCGTATTATGCCCCTGTGCCAGAACAACCGGTGGCAGGTAACGCCTGGCAAGCCGAAGAGCAGCAATCCACttt                      <  1:175513/81‑1 (MQ=255)                                                   aaCAGCCGTATTATGCCCCTGTGCCAGAACAACCGGTGGCAGGTAACGCCTGGCAAGCCGAAGAGCAGCAATCCACTTTTg                    >  2:175535/1‑81 (MQ=255)                                                    aCAGCCGTATTATGCCCCTGTGCCAGAACAACCGGTGGCAGGTAACGCCTGGCAAGCCGAAGAGCAGCAATCCACTTTTGc                   >  1:175551/1‑81 (MQ=255)                                                      aGCCGTATTATGCCCCTGTGCCAGAACAACCGGTGGCAGGTAACGCCTGGCAAGCCGAAGAGCAGCAATCCACTTTTGCTc                 <  2:175514/81‑1 (MQ=255)                                                        ccGTATTATGCCCCTGTGCCAGAACAACCGGTGGCAGGTAACGCCTGGCAAGCCGAAGAGCAGCAATCCACTTTTGCTCca               <  1:175515/81‑1 (MQ=255)                                                           tattatGCCCCTGTGCCAGAACAACCGGTGGCAGGTAACGCCTGGCAAGCCGAAGAGCAGCAATCCACTTTTGCTCCACAg            <  1:175516/81‑1 (MQ=255)                                                               atGCCCCTGTGCCAGAACAACCGGTGGCAGGTAACGCCTGGCAAGCCGAAGAGCAGCAATCCACTTTTGCTCCACAGTCt         <  1:175517/80‑1 (MQ=255)                                                                     cTGTGCCAGAACAACCGGTGGCAGGTAACGCCTGGCAAGCCGAAGAGCAGCAATCCACTTTTGCTCCACAGTCTACATAcc  <  2:175518/81‑1 (MQ=255)                                                                        |                                                                              AGTACAGCCGCAGCAGCCGTATTATGCACCTGCAGCTGAACAACCTGCGCAACAGCCGTATTATGCCCCTGCGCCAGAACAACCGGTGGCAGGTAACGCCTGGCAAGCCGAAGAGCAGCAATCCACTTTTGCTCCACAGTCTACATACC  >  NC_000913/934423‑934571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑