Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	935,242	A→G	V673V (GTA→GTG)	ftsK →	cell division DNA translocase FtsK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	935,242	0	A	G	100.0%	119.2 / NA	35	V673V (GTA→GTG)	ftsK	cell division DNA translocase FtsK
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/13);  total (22/13)

GAACTGGCACGTCAGTTCGCCCAGACACAGCAGCAACGCTATGGCGAACAGTATCAACATGATGTGCCCGTAAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTCGTCAGTTTGCGCAAACTCAACAACAACGTTAT  >  NC_000913/935171‑935317                                                                        |                                                                            gAACTGGCACGTCAGTTCGCCCAGACACAGCAGCAACGCTATGGCGAACAGTATCAACATGATGTGCCCGTGAACGCag                                                                      >  2:175686/1‑79 (MQ=255)  aaCTGGCACGTCAGTTCGCCCAGACACAGCAGCAACGCTATGGCGAACAGTATCAACATGATGTGCCCGTGAACGCagaag                                                                   <  2:175634/81‑1 (MQ=255)  aaCTGGCACGTCAGTTCGCCCAGACACAGCAGCAACGCTATGGCGAACAGTATCAACATGATGTGCCCGTGAACGCag                                                                      >  1:175665/1‑78 (MQ=255)  aaCTGGCACGTCAGTTCGCCCAGACACAGCAGCAACGCTATGGCGAACAGTATCAACATGATGTGCCCGTGAACGCag                                                                      >  1:175674/1‑78 (MQ=255)  aaCTGGCACGTCAGTTCGCCCAGACACAGCAGCAACGCTATGGCGAACAGTATCAACATGATGTGCCCGTGAACGCag                                                                      >  1:175675/1‑78 (MQ=255)    cTGGCACGTCAGTTCGCCCAGACACAGCAGCAACGCTATGGCGAACAGTATCAACATGATGTGCCCGTGAACGCAGAagat                                                                 >  2:175678/1‑81 (MQ=255)    cTGGCACGTCAGTTCGCCCAGACACAGCAGCAACGCTATGGCGAACAGTATCAACATGATGTGCCCGTGAACGCAGAagat                                                                 >  1:175679/1‑81 (MQ=255)      ggCACGTCAGTTCGCCCAGACACAGCAGCAACGCTATGGCGAACAGTATCAACATGATGTGCCCGTGAACGCAGAagatg                                                                >  1:175685/1‑80 (MQ=255)       gCACGTCAGTTCGCCCAGACACAGCAGCAACGCTATGGCGAACAGTATCAACATGATGTGCCCGTGAACGCAGAagatgc                                                               <  2:175635/80‑1 (MQ=255)       gCACGTCAGTTCGCCCAGACACAGCAGCAACGCTATGGCGAACAGTATCAACATGATGTGCCCGTGAACGCAGAagatg                                                                >  1:175677/1‑79 (MQ=255)           gTCAGTTCGCCCAGACACAGCAGCAACGCTATGGCGAACAGTATCAACATGATGTGCCCGTGAACGCAGAagatgcagatg                                                          <  1:175636/81‑1 (MQ=255)              aGTTCGCCCAGACACAGCAGCAACGCTATGGCGAACAGTATCAACATGATGTGCCCGTGAACGCAGAAGATGCAGAtgct                                                        <  1:175637/80‑1 (MQ=255)               gtacGCCCAGACACAGCAGCAACGCTATGGCGAACAGTATCAACATGATGTGCCCGTGAACGCAGAAGATGCAGAtgctgc                                                      <  2:175638/78‑1 (MQ=255)                      cAGACACAGCAGCAACGCTATGGCGAACAGTATCAACATGATGTGCCCGTGAACGCAGAAGATGCAGATGCTGCGGCagag                                               >  1:175684/1‑81 (MQ=255)                         acacAGCAGCAACGCTATGGCGAACAGTATCAACATGATGTGCCCGTGAACGCAGAAGATGCAGATGCTGCGGCAGAGGCt                                            >  2:175683/1‑81 (MQ=255)                              gcagcaACGCTATGGCGAACAGTATCAACATGATGTGCCCGTGAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACt                                       <  2:175639/81‑1 (MQ=255)                                agcaACGCTATGGCGAACAGTATCAACATGATGTGCCCGTGAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTgg                                     <  1:175640/81‑1 (MQ=255)                                      gCTATGGCGAACAGTATCAACATGATGTGCCCGTGAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTCGt                                >  1:175693/1‑80 (MQ=255)                                      gCTATGGCGAACAGTATCAACATGATGTGCCCGTGAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTCGTc                               <  2:175641/81‑1 (MQ=255)                                      gCTATGGCGAACAGTATCAACATGATGTGCCCGTGAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTCGTc                               <  2:175642/81‑1 (MQ=255)                                       cTATGGCGAACAGTATCAACATGATGTGCCCGTGAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTCGTCa                              <  2:175643/81‑1 (MQ=255)                                         aTGGCGAACAGTATCAACATGATGTGCCCGTGAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTCGTCAGt                            >  2:175690/1‑81 (MQ=255)                                               aaCAGTATCAACATGATGTGCCCGTGAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTCGTCAGTTTgcgc                      <  2:175644/81‑1 (MQ=255)                                                aCAGTATCAACATGATGTGCCCGTGAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTCGTCAGTTTgcgc                      >  2:175681/1‑80 (MQ=255)                                                aCAGTATCAACATGATGTACCCGTGAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTCGTCAGTTTgcgc                      >  1:175697/1‑80 (MQ=255)                                                         aCATGATGTGCCCGTGAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTAGTCAGTTTGCGCAAACTcaac             >  2:175695/1‑80 (MQ=255)                                                          cATGATGTGCCCGTGAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTCGTCAGTTTGCGCAAACTcaacaa           >  1:175676/1‑81 (MQ=255)                                                          cATGATGTGCCCGTGAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTCGTCAGTTTGCGCAAACTcaacaa           >  2:175687/1‑81 (MQ=255)                                                            tgatgTGCCCGTGAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTCGTCAGTTTGCGCAAACTcaacaac          >  1:175698/1‑80 (MQ=255)                                                             gatgTGCCCGCGAACGCAGAAGATGCAGATGATGCGGCAGAGGCTGAACTGGCTCGACTGTTTGTGCAAACTccacaacac        >  2:175688/1‑80 (MQ=255)                                                              atgTGCCCGTGAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTCGTCAGTTTGCGCAAACTCaacaacaac       >  1:175689/1‑81 (MQ=255)                                                                 tgCCCGTGAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTCGTCAGTTTGCGCAAACTCAACAACAACGtt    <  1:175645/81‑1 (MQ=255)                                                                 tgCCCGTGAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTCGTCAGTTTGCGCAAACTCAACAACAACGt     >  2:175701/1‑80 (MQ=255)                                                                  gCCCGTGAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTCGTCAGTTTGCGCAAACTCAACAACAACGTTa   >  1:175682/1‑81 (MQ=255)                                                                   cccGTGAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTCGTCAGTTTGCGCAAACTCAACAACAACGTTAt  <  2:175646/81‑1 (MQ=255)                                                                        |                                                                            GAACTGGCACGTCAGTTCGCCCAGACACAGCAGCAACGCTATGGCGAACAGTATCAACATGATGTGCCCGTAAACGCAGAAGATGCAGATGCTGCGGCAGAGGCTGAACTGGCTCGTCAGTTTGCGCAAACTCAACAACAACGTTAT  >  NC_000913/935171‑935317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑