Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	104,426	C→T	L149F (CTT→TTT)	ftsA →	cell division protein FtsA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	104,426	0	C	T	100.0%	152.1 / NA	42	L149F (CTT→TTT)	ftsA	cell division protein FtsA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/17);  total (25/17)

ATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGAAAAACATCGTCAAAGCG  >  NC_000913/104355‑104503                                                                        |                                                                              aTCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCg                                                                      >  1:23114/1‑81 (MQ=255)   cGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTg                                                                    >  2:23088/1‑81 (MQ=255)    gtgtGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGc                                                                   <  1:23037/81‑1 (MQ=255)       tgctgcATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCgg                                                                 <  1:23038/80‑1 (MQ=255)         ctgcATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATg                                                              <  1:23039/81‑1 (MQ=255)          tgcATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATg                                                              >  2:23092/1‑80 (MQ=255)           gcATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATg                                                              >  1:23096/1‑79 (MQ=255)            cATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAg                                                           <  1:23040/81‑1 (MQ=255)                  aTCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCaaaa                                                     <  1:23041/81‑1 (MQ=255)                     ccGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTg                                                  <  2:23042/81‑1 (MQ=255)                       gCAAGAGTATGCGATTGACTATCAGGAAGGGGTCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCa                                                >  1:23094/1‑81 (MQ=255)                         aaGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCAcc                                              <  2:23043/81‑1 (MQ=255)                         aaGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCAcc                                              >  1:23097/1‑81 (MQ=255)                           gagTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCt                                             >  2:23093/1‑80 (MQ=255)                           gagTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTg                                            <  1:23044/81‑1 (MQ=255)                            agTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGa                                           >  2:23091/1‑81 (MQ=255)                            agTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGa                                           >  2:23113/1‑81 (MQ=255)                             gTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGAt                                          <  1:23045/81‑1 (MQ=255)                              tATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATc                                         <  1:23046/81‑1 (MQ=255)                               aTGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATca                                        >  2:23090/1‑81 (MQ=255)                                  cGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACAt                                     >  2:23110/1‑81 (MQ=255)                                  cGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACAt                                     >  1:23115/1‑81 (MQ=255)                                   gATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATg                                    >  1:23100/1‑81 (MQ=255)                                       gACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTca                                 <  1:23047/80‑1 (MQ=255)                                       gACTATCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTca                                 >  1:23101/1‑80 (MQ=255)                                           aTCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAAc                             >  2:23089/1‑80 (MQ=255)                                            tCAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAAc                             <  2:23048/79‑1 (MQ=255)                                             cAGGAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGa                           >  2:23104/1‑80 (MQ=255)                                                gAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATg                       >  1:23119/1‑81 (MQ=255)                                                gAAGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATg                       <  2:23049/81‑1 (MQ=255)                                                 aaGGGATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATgg                      <  1:23050/81‑1 (MQ=255)                                                    ggATCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCg                    >  2:23098/1‑80 (MQ=255)                                                       tCAAGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGaaaa                <  1:23051/81‑1 (MQ=255)                                                         aaGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGaaaaa               >  2:23129/1‑80 (MQ=255)                                                         aaGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGaaaaa               >  1:23117/1‑80 (MQ=255)                                                         aaGAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGaaaaa               >  1:23111/1‑80 (MQ=255)                                                           gAATCCGGTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGAAAAACAt            >  2:23121/1‑81 (MQ=255)                                                                 ggTAGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACACGTCACAACGATATGGCGAAAAACATCGTCa       >  2:23102/1‑80 (MQ=255)                                                                    aGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGAAAAACATCGTCAAAGc   >  1:23108/1‑81 (MQ=255)                                                                    aGGATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGAAAAACATCGTCAAAGc   >  2:23127/1‑81 (MQ=255)                                                                     ggATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGAAAAACATCGTCAAAGc   <  1:23052/80‑1 (MQ=255)                                                                     ggATTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGAAAAACATCGTCAAAGCg  <  1:23053/81‑1 (MQ=255)                                                                        |                                                                              ATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGAAAAACATCGTCAAAGCG  >  NC_000913/104355‑104503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑