Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	978,267	G→A	E648K (GAA→AAA)	mukB →	chromosome partitioning protein MukB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	978,267	0	G	A	100.0%	99.6 / NA	28	E648K (GAA→AAA)	mukB	chromosome partitioning protein MukB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/17);  total (11/17)

ACCTCCAGCCAGGACGTCACCGAATATCTGCAACAGTTGCTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATGAAGTGGGCGCGCGCAAAAACGCCGTCGATGAAGAGATCGAACGTTTAAGCCAGCCTGGCGGCTCTGAAGATC  >  NC_000913/978192‑978339                                                                            |                                                                         aCCTCCAGCCAGGACGTCACCGAATATCTGCAACAGTTGCTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATAAAGt                                                                      <  2:182568/80‑1 (MQ=255)  ccTCCAGCCAGGACGTCACCGAATATCTGCAACAGTTGCTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATAAAGTgg                                                                    <  2:182569/81‑1 (MQ=255)   cTCCAGCCAGGACGTCACCGAATATCTGCAACAGTTGCTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATAAAGTggg                                                                   <  1:182570/81‑1 (MQ=255)       agccagGACGTCACCGAATATCTGCAACAGTTGCTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATAAAGTGGgcgcg                                                               >  1:182628/1‑81 (MQ=255)                  cACCGAATATCTGCAACAGTTGCTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATAAAGTGGGCGCGCGCAAAAACGc                                                    >  2:182609/1‑81 (MQ=255)                    ccGAATATCTGCAACAGTTGCTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATAAAGTGGGCGCGCGCAAAAACGCCg                                                  >  1:182608/1‑81 (MQ=255)                      gAATATCTGCAACAGTTGCTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATAAAGTGGGCGCGCGCAAAAACGCCGTc                                                >  2:182615/1‑81 (MQ=255)                         tatCTGCAACAGTTGCTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATAAAGTGGGCGCGCGCAAAAACGCCGTCGAt                                             <  2:182571/81‑1 (MQ=255)                               cAACAGTTGCTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATAAAGTGGGCGCGCGCAAAAACGCCGTCGATGaa                                          <  1:182574/78‑1 (MQ=255)                               cAACAGTTGCTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATAAAGTGGGCGCGCGCAAAAACGCCGTCGATGAag                                         <  1:182572/79‑1 (MQ=255)                               cAACAGTTGCTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATAAAGTGGGCGCGCGCAAAAACGCCGTCGATGAag                                         <  1:182573/79‑1 (MQ=255)                                 aCAGTTGCTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATAAAGTGGGCGCGCGCAAAAACCCCGTCGATGaa                                          >  2:182619/1‑76 (MQ=255)                                  cAGTTGCTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATAAAGTGGGCGCGCGCAAAAACGCCGTCGATGAAGAGATc                                    <  1:182575/81‑1 (MQ=255)                                   aGTTGCTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATAAAGTGGGCGCGCGCAAAAACGCCGTCGATGAAGAGATc                                    >  1:182625/1‑80 (MQ=255)                                   aGTTGCTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATAAAGTGGGCGCGCGCAAAAACGCCGTCGATGAAGAGATCg                                   <  1:182576/81‑1 (MQ=255)                                        cTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATAAAGTGGGCGCGCGCAAAAACGCCGTCGATGAAGAGATCGAACGt                              <  2:182577/81‑1 (MQ=255)                                        cTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATAAAGTGGGCGCGCGCAAAAACGCCGTCGATGAAGAGATCGAACGt                              <  1:182579/81‑1 (MQ=255)                                        cTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATAAAGTGGGCGCACGCAAAAACGCCGTCGATGAAGAGATCGAACGt                              <  1:182578/81‑1 (MQ=255)                                           gAGCGTGAGCGAGAGGCGATTGTTGAACGCGATAAAGTGGGCGCGCGCAAAAACGCCGTCGATGAAGAGATCGAACGctt                            <  2:182580/80‑1 (MQ=255)                                             gCGTGAGCGAGAGGCGATTGTTGAACGCGATAAAGTGGGCGCGCGCAAAAACGCCGTCGATGAAGAGATCGAACGTTTAag                         >  1:182600/1‑81 (MQ=255)                                                  aGCGAGAGGCGATTGTTGAACGCGATAAAGTGGGCGCGCGCAAAAACGCCGTCGATGAAGAGATCGAACGTTTAagccagc                    <  1:182581/81‑1 (MQ=255)                                                    cGAGAGGCGATTGTTGAACGCGATAAAGTGGGCGCGCGCAAAAACGCCGTCGATGAAGAGATCGAACGTTTAAGCCAGCCt                  <  1:182582/81‑1 (MQ=255)                                                     gagaGGCGATTGTTGAACGCGATAAAGTGGGCGCGCGCAAAAACGCCGTCGATGAAGAGATCGAACGTTTAAGCCAGCCTg                 >  2:182624/1‑81 (MQ=255)                                                       gagGCGATTGTTGAACGCGATAAAGTGGGCGCGCGCAAAAACGCCGTCGATGAAGAGATCGAACGTTTAAGCCAGCCTggc               >  2:182613/1‑81 (MQ=255)                                                        agGCGATTGTTGAACGCGATAAAGTGGGCGCGCGCAAAAACGCCGTCGATGAAGAGATCGAACGTTTAAGCCAGCCTggcg              >  2:182612/1‑81 (MQ=255)                                                              ttgttgAACGCGATAAAGTGGGCGCGCGCAAAAACGCCGTCGATGAAGAGATCGAACGTTTAAGCCAGCCTGGCGGctct         >  1:182616/1‑80 (MQ=255)                                                                    aaCGCGATAAAGTGGGCGCGCGCAAAAACGCCGTCGATGAAGAGATCGAACGTTTAAGCCAGCCTGGCGGCTCTGAAGAt   <  2:182584/80‑1 (MQ=255)                                                                    aaCGCGATAAAGTGGGCGCGCGCAAAAACGCCGTCGATGAAGAGATCGAACGTTTAAGCCAGCCTGGCGGCTCTGAAGATc  <  1:182583/81‑1 (MQ=255)                                                                            |                                                                         ACCTCCAGCCAGGACGTCACCGAATATCTGCAACAGTTGCTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATGAAGTGGGCGCGCGCAAAAACGCCGTCGATGAAGAGATCGAACGTTTAAGCCAGCCTGGCGGCTCTGAAGATC  >  NC_000913/978192‑978339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑