Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	981,907	C→A	S287S (TCC→TCA)	ldtD →	L,D‑transpeptidase LdtD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	981,907	0	C	A	100.0%	73.8 / NA	21	S287S (TCC→TCA)	ldtD	L,D‑transpeptidase LdtD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (14/7);  total (14/7)

GGCATGTTGGACGGGGGGCCGAAAATTACTCTACCTGGCGATGACACGCCAACTGACGCGGTAGTCAGCCCATCCGCTGTTACTGTTGAAACAGCAGAAACTAAGCCGATGGATAAGCAAACGACGTCTCGTAGTAAACCTGCGCCTGCCG  >  NC_000913/981833‑981983                                                                           |                                                                             ggCATGTTGGACGGGGGGCCGAAAATTACTCTACCTGGCGATGACACGCCAACTGACGCGGTAGTCAGCCCATCAGCTGtt                                                                        <  2:183170/81‑1 (MQ=255)    aTGTTGGACGGGGGGCCGAAAATTACTCTACCTGGCGATGACACGCCAACTGACGCGGTAGTCAGCCCATCAGCTGTTACt                                                                     >  2:183193/1‑81 (MQ=255)         ggACGGGGGGCCGAAAATTACTCTACCTGGCGATGACACGCCAACTGACGCGGTAGTCAGCCCATCAGCTGTTACTGTTg                                                                 >  1:183210/1‑80 (MQ=255)            cGGGGGGCCGAAAATTACTCTACCTGGCGATGACACGCCAACTGACGCGGTAGTCAGCCCATCAGCTGTTACTGTTGAAAc                                                             <  2:183171/81‑1 (MQ=255)                      aaaaTTACTCTACCTGGCGATGACACGCCAACTGACGCGGTAGTCAGCCCATCAGCTGTTACTGTTGAAACAGCAGAAAc                                                    >  1:183204/1‑80 (MQ=255)                         aTTACTCTACCTGGCGATGACACGCCAACTGACGCGGTAGTCAGCCCATCAGCTGTTACTGTTGAAACAGCAGAAACTAAg                                                <  2:183172/81‑1 (MQ=255)                          ttACTCTACCTGGCGATGACACGCCAACTGACGCGGTAGTCAGCCCATCAGCTGTTACTGTTGAAACAGCAGAAACTAAGc                                               >  2:183192/1‑81 (MQ=255)                                tACCTGGCGATGACACGCCAACTGACGCGGTAGTCAGCCCATCAGCTGTTACTGTTGAAACAGCAGAAACTAAGCCGATg                                          >  2:183208/1‑80 (MQ=255)                                 aCCTGGCGATGACACGCCAACTGACGCGGTAGTCAGCCCATCAGCTGTTACTGTTGAAACAGCAGAAACTAAGCCGATGGa                                        >  1:183203/1‑81 (MQ=255)                                 aCCTGGCGATGACACGCCAACTGACGCGGTAGTCAGCCCATCAGCTGTTACTGTTGAAACAGCAGAAACTAAGCCGATGGa                                        >  1:183206/1‑81 (MQ=255)                                  ccTGGCGATGACACGCCAACTGACGCGGTAGTCAGCCCATCAGCTGTTACTGTTGAAACAGCAGAAACTAAGCCGATGGa                                        <  1:183173/80‑1 (MQ=255)                                   cTGGCGATGACACGCCAACTGACGCGGTAGTCAGCCCATCAGCTGTTACTGTTGAAACAGCAGAAACTAAGCCGATGGAt                                       >  1:183200/1‑80 (MQ=255)                                      gCGATGACACGCCAACTGACGCGGTAGTCAGCCCATCAGCTGTTACTGTTGAAACAGCAGAAACTAAGCCGATGGATAAGc                                   <  2:183174/81‑1 (MQ=255)                                        gATGACACGCCAACTGACGCGGTAGTCAGCCCATCAGCTGTTACTGTTGAAACAGCAGAAACTAAGCCGATGGATAAGCa                                  >  1:183209/1‑80 (MQ=255)                                        gATGACACGCCAACTGACGCGGTAGTCAGCCCATCAGCTGTTACTGTTGAAACAGCAGAAACTAAGCCGATGGATAAGCa                                  >  1:183202/1‑80 (MQ=255)                                          tGACACGCCAACTGACGCGGTAGTCAGCCCATCAGCTGTTACTGTTGAAACAGCAGAAACTAAGCCGATGGATAAGCAAac                               >  2:183199/1‑81 (MQ=255)                                                    aCTGACGCGGTAGTCAGCCCATCAGCTGTTACTGTTGAAACAGCAGAAACTAAGCAGATGGATAAGCAAACGACGTCTCgt                     <  2:183175/81‑1 (MQ=255)                                                       gACGCGGTAGTCAGCCCATCAGCTGTTACTGTTGAAACAGCAGAAACTAAGCCGATGGATAAGCAAACGACGTCTCgtag                   >  2:183216/1‑80 (MQ=255)                                                             gTAGTCAGCCCATCAGCTGTTACTGTTGAAACAGCAGAAACTAAGCCGATGGATAAGCAAACGACGTCTCGTAGTAAACCt            >  1:183211/1‑81 (MQ=255)                                                                      ccATCAGCTGTTACTGTTGAAACAGCAGAAACTAAGCCGATGGATAAGCAAACGACGTCTCGTAGTAAACCTGCGCCTGcc   <  2:183176/81‑1 (MQ=255)                                                                       cATCAGCTGTTACTGTTGAAACAGCAGAAACTAAGCCGATGGATAAGCAAACGACGTCTCGTAGTAAACCTGCGCCTGCCg  >  2:183207/1‑81 (MQ=255)                                                                           |                                                                             GGCATGTTGGACGGGGGGCCGAAAATTACTCTACCTGGCGATGACACGCCAACTGACGCGGTAGTCAGCCCATCCGCTGTTACTGTTGAAACAGCAGAAACTAAGCCGATGGATAAGCAAACGACGTCTCGTAGTAAACCTGCGCCTGCCG  >  NC_000913/981833‑981983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑