Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	982,788	T→C	V581A (GTT→GCT)	ldtD →	L,D‑transpeptidase LdtD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	982,788	0	T	C	100.0%	69.9 / NA	21	V581A (GTT→GCT)	ldtD	L,D‑transpeptidase LdtD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/11);  total (10/11)

CGCTGAAGCAGGGTGATACACGTTACGTCAATATTCGGCAGTCGATTCCGGTGAATCTCTACTACCTGACGGCCTTTGTTGGTGCAGATGGTCGTACCCAGTATCGTACAGATATTTACAATTATGATCTGCCTGCGCGATCCAGCTCGCAAAT  >  NC_000913/982710‑982863                                                                               |                                                                            cgcTGAAGCAGGGTGATACACGTTACGTCAATATTCGGCAGTCGATTCCGGTGAATCTCTACTACCTGACGGCCTttgctg                                                                           >  2:183354/1‑81 (MQ=255)      aaGCAGGGTGATACACGTTACGTCAATATTCGGCAGTCGATTCCGGTGAATCTCTACTACCTGACGGCCTTTGCTGGTg                                                                        >  2:183341/1‑79 (MQ=255)      aaGCAGGGTGATACACGTTACGTCAATATTCGGCAGTCGATTCCGGTGAATCTCTACTACCTGACGGCCTTTGCTGGTg                                                                        <  2:183319/79‑1 (MQ=255)       aGCAGGGTGATACACGTTACGTCAATATTCGGCAGTCGATTCCGGTGAATCTCTACTACCTGACGGCCTTTGCTGGTg                                                                        >  2:183339/1‑78 (MQ=255)              tGATACACGTTACGTCAATATTCGGCAGTCGATTCCGGTGAATCTCTACTACCTGACGGCCTTTGCTGGTGCAGATGGTCg                                                              <  1:183320/81‑1 (MQ=255)                       ttACGTCAATATTCGGCAGTCGATTCCGGTGAATCTCTACTACCTGACGGCCTTTGCTGGTGCAGATGGTCGTACCCAGTa                                                     >  1:183340/1‑81 (MQ=255)                        tACGTCAATATTCGGCAGTCGATTCCGGTGAATCTCTACTACCTGACGGCCTTTGCTGGTGCAGATGGTCGTACCCAGTAt                                                    >  2:183356/1‑81 (MQ=255)                              aaTATTCGGCAGTCGATTCCGGTGAATCTCTACTAGCTGACGGCCTTTGCTGGTGCAGATGGTCGTACCCAGTATCGTACa                                              <  2:183321/81‑1 (MQ=255)                              aaTATTCGGCAGTCGATTCCGGTGAATCTCTACTACCTGACGGCCTTTGCTGGTGCAGATGGTCGTACCCAGTATCGTa                                                >  1:183347/1‑79 (MQ=255)                                     ggCAGTCGATTCCGGTGAATCTCTACTACCTGACGGCCTTTGCTGGTGCAGATGGTCGTACCCAGTATCGTACAGATAtt                                        <  1:183322/80‑1 (MQ=255)                                     ggCAGTCGATTCCGGTGAATCTCTACTACCTGACGGCCTTTGCTGGTGCAGATGGTCGTACCCAGTATCGTACAGATAtt                                        >  1:183350/1‑80 (MQ=255)                                        aGTCGATTCCGGTGAATCTCTACTACCTGACGGCCTTTGCTGGTGCAGATGGTCGTACCCAGTATCGTACAGATATTTACa                                    <  2:183323/81‑1 (MQ=255)                                            gATTCCGGTGAATCTCTACTACCTGACGGCCTTTGCTGGTGCAGATGGTCGTACCCAGTATCGTACAGATATTTACAATTa                                >  2:183344/1‑81 (MQ=255)                                               tCCGGTGAATCTCTACTACCTGACGGCCTTTGCTGGTGCAGATGGTCGTACCCAGTATCGTACAGATATTTACAATTATGa                             >  2:183349/1‑81 (MQ=255)                                                ccGGTGAATCTCTACTACCTGACGGCCTTTGCTGGTGCAGATGGTCGTACCCAGTATCGTACAGATATTTACAATTATGa                             <  1:183324/80‑1 (MQ=255)                                                     gAATCTCTACTACCTGACGGCCTTTGCTGGTGCAGATGGTCGTACCCAGTATCGTACAGATAGTTAAAATTATGATctgcc                       <  2:183325/81‑1 (MQ=255)                                                            tactacCTGACGGCCTTTGCTGGTGCAGATGGTCGTACCCAGTATCGTACAGATATTTACAATTATGATctgcctgc                    <  2:183326/77‑1 (MQ=255)                                                            tactacCTGACGGCCTTTGCTGGTGCAGATGGTCGTACCCAGTATCGTACAGATATTTACAATTATGATCTGCCTgcg                   <  1:183327/78‑1 (MQ=255)                                                            tactacCTGACGGCCTTTGCTGGTGCAGATGGTCGTACCCAGTATCGTACAGATATTTACAATTATGATCTGCCTGCGCGa                >  1:183355/1‑81 (MQ=255)                                                                     aCGGCCTTTGCTGGTGCAGATGGTCGTACCCAGTATCGTACAGATATTTACAATTATGATCTGCCTGCGCGATCCAGCt         <  1:183328/79‑1 (MQ=255)                                                                        gCCTTTGCTGGTGCAGATGGTCGTACCCAGTATCGTACAGATATTTACAATTATGATCTGCCTGCGCGATCCAGCTCGCaa    <  2:183329/81‑1 (MQ=255)                                                                          cTTTGCTGGTGCAGATGGTCGTACCCAGTATCGTACAGATATTTACAATTATGATCTGCCTGCGCGATCCAGCTCGCAAAt  >  2:183365/1‑81 (MQ=255)                                                                               |                                                                            CGCTGAAGCAGGGTGATACACGTTACGTCAATATTCGGCAGTCGATTCCGGTGAATCTCTACTACCTGACGGCCTTTGTTGGTGCAGATGGTCGTACCCAGTATCGTACAGATATTTACAATTATGATCTGCCTGCGCGATCCAGCTCGCAAAT  >  NC_000913/982710‑982863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑