Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	989,174	G→A	H395Y (CAT→TAT)	pncB ←	nicotinate phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	989,174	0	G	A	100.0%	81.1 / NA	23	H395Y (CAT→TAT)	pncB	nicotinate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/8);  total (15/8)

GCGAAAGATTTCGGAATTAAAGGTAAAAAAAGGGAGCCGATTAGCTCCCTGATGATATTAACTGGCTTTTTTAATATGCGGAAGGTCGAACGCTTTGCGCAGCGCCCGAACAAACGCTTTATCATGGCAGATAGTTTTGCCAGG  >  NC_000913/989097‑989240                                                                              |                                                                   gCGAAAGATTTCGGAATTAAAGGTAAAAAAAGGGAGCCGATTAGCTCCCTGATGATATTAACTGGCTTTTTTAATATACg                                                                  >  1:184301/1‑80 (MQ=255)     aaGATTTCGGAATTAAAGGTAAAAAAAGGGAGCCGATTAGCTCCCTGATGATATTAACTGGCTTTTTTAATATACGGAAgg                                                             <  1:184275/81‑1 (MQ=255)      aGATTTCGGAATTAAAGGTAAAAAAAGGGAGCCGATTAGCTCCCTGATGATATTAACTGGCTTTTTTAATATACGGAAGGt                                                            >  1:184310/1‑81 (MQ=255)          ttCGGAATTAAAGGTAAAAAAAGGGAGCCGATTAGCTCCCTGATGATATTAACTGGCTTTTTTAATATACGGAAGGTCGaa                                                        >  1:184296/1‑81 (MQ=255)            cGGAATTAAAGGTAAAAAAAGGGAGCCGATTAGCTCACTGATGATATTAACTGGCTTTTTTAATATACGGAAGGTCGAACg                                                      >  1:184303/1‑81 (MQ=255)                aTTAAAGGTAAAAAAAGGGAGCCGATTAGCTCCCTGATGATATTAACTGGCTTTTTTAATATACGGAAGGTCGAACGCttt                                                  >  1:184308/1‑81 (MQ=255)                   aaaGGTAAAAAAAGGGAGCCGATTAGCTCCCTGATGATATTAACTGGCTTTTTTAATATACGGAAGGTCGAACGCTTTgcg                                               >  2:184316/1‑81 (MQ=255)                    aaGGTAAAAGAAGGGAGCCGATTAGCTCCCTGATGATATTAACTGGCTTTTTTAATATACGGAAGGTCGAACGCTTTgcg                                               >  1:184287/1‑80 (MQ=255)                     aGGTAAAAAAAGGGAGCCGATCAGCTCCCTGATGATATTAACTGGCTTTTTTAATATACGGAAGGTCGAACGCTTTGCGCa                                             <  1:184276/81‑1 (MQ=255)                           aaaaaGGGAGCCGATTAGCTCCCTGATGATATTAACTGGCTTTTTTAATATACGGAAGGTCGAACGCTTTGCGCAGCGccc                                       <  1:184277/81‑1 (MQ=255)                            aaaaGGGAGCCGATTAGCTCCCTGATGATATTAACTGGCTTTTTTAATATACGGAAGGTCGAACGCTTTGCGCAGCGCCCg                                      <  2:184278/81‑1 (MQ=255)                                 ggAGCCGATTAGCTCCCTGATGATATTAACTGGCTTTTTTAATATACGGAAGGTCGAACGCTTTGCGCAGCGCCCGAACaa                                 <  1:184279/81‑1 (MQ=255)                                  gAGCCGATTAGCTCCCTGATGATATTAACTGGCTTTTTTAATATACGGAAGGTCGAACGCTTTGCGCAGCGCCCGAACaaa                                >  1:184312/1‑81 (MQ=255)                                     ccGATTAGCTCCCTGATGGTATTAACTGGCTTTTTTAATATACGGAAGGTCGAACGCTTTGCGCAGCGCCCGAACAAACGc                             >  1:184313/1‑81 (MQ=255)                                        aTTAGCTCCCTGATGATATTAACTGGCTTTTTTAATATACGGAAGGTCGAACGCTTTGCGCAGCGCCCGAACAAACGCtt                           >  1:184311/1‑80 (MQ=255)                                        aTTAGCTCCCTGATGATATTAACTGGCTTTTTTAATATACGGAAGGTCGAACGCTTTGCGCAGCGCCCGAACAAACGCtt                           >  1:184320/1‑80 (MQ=255)                                             cTCCCTGATGATATTAACTGGCTTTTTTAATATACGGAAGGTCGAACGCTTTGCGCAGCGCCCGAACAAACGCTTTATCAt                     <  1:184280/81‑1 (MQ=255)                                                    atgatATTAACTGGCTTTTTTAATATACGGAGGGTCGAACGCTTTGCGCAGCGCCCGAACAAACGCTTTATCATGGCAGAt              <  2:184281/81‑1 (MQ=255)                                                    atgatATTAACTGGCTTTTTTAATATACGGAAGGTCGAACGCTTTGCGCAGCGCCCGAACAAACGCTTTATCATGGCAGa               >  2:184325/1‑80 (MQ=255)                                                        tatTAACTGGCTTTTTTAATATACGGAAGGTCGAACGCTTTGCGCAGCGCCCGAACAAACGCTTTATCATGGCAGATAGtt          >  2:184309/1‑81 (MQ=255)                                                            aaCTGGCTTTTTTAATATACGGAAGGTCGAACGCTTTGCGCAGCGCCCGAACAAACGCTTTATCATGGCAGATAGTTTTGc      >  2:184315/1‑81 (MQ=255)                                                              cTGGCTTTTTTAATATACGGAAGGTCGAACGCTTTGCGCAGCGCCCGAACAAACGCTTTATCATGGCAGATAGTTTTGc      >  2:184319/1‑79 (MQ=255)                                                                 gCTTTTTTAATATACGGAAGGTCGAACGCTTTGCGCAGCGCCCGAACAAACGCTTTATCATGGCAGATAGTTTTGCCAgg  <  2:184282/80‑1 (MQ=255)                                                                              |                                                                   GCGAAAGATTTCGGAATTAAAGGTAAAAAAAGGGAGCCGATTAGCTCCCTGATGATATTAACTGGCTTTTTTAATATGCGGAAGGTCGAACGCTTTGCGCAGCGCCCGAACAAACGCTTTATCATGGCAGATAGTTTTGCCAGG  >  NC_000913/989097‑989240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑