Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	990,420	G→A	intergenic (‑64/‑202)	pncB ← / → pepN	nicotinate phosphoribosyltransferase/aminopeptidase N

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	990,420	0	G	A	100.0%	96.6 / NA	27	intergenic (‑64/‑202)	pncB/pepN	nicotinate phosphoribosyltransferase/aminopeptidase N
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/15);  total (12/15)

GTGTCATAGGTGCGCAGTAGCGTCCTCGTACAGGAGCGTTTAGTACAATAAACATCTTCAGGAACTGCTGGAGTATACCTTGTTTCGCAATTTATTGAACCCCGATCACACCATATGCCACCTTTCTGGTCGATGGCATTACGCGGTG  >  NC_000913/990350‑990497                                                                       |                                                                              gtgtCATAGGTGCGCAGTAGCGTCCTCGTACAGGAGCGTTTAGTACAATAAACATCTTCAGGAACTGCTGAAGTATACCt                                                                      <  1:184485/80‑1 (MQ=255)    tCATAGGTGCGCAGTAGCGTCCTCGTACAGGAGCGTTTAGTACAATAAACATCTTCAGGAACTGCTGAAGTATACCTTGt                                                                   <  1:184486/80‑1 (MQ=255)      aTAGGTGCGCAGTAGCGTCCTCGTACAGGAGCGTTTAGTACAATAAACATCTTCAGGAACTGCTGAAGTATACCTTGTTTc                                                                >  2:184519/1‑81 (MQ=255)      aTAGGTGCGCAGTAGCGTCCTCGTACAGGAGCGTTTAGTACAATAAACATCTTCAGGAACTGCTGAAGTATACCTTGTTTc                                                                >  1:184529/1‑81 (MQ=255)           tGCGCAGTAGCGTCCTCGTACAGGAGCGTTTAGTACAATAAACATCTTCAGGAACTGCTGAAGTATACCTTGTTTCGCAAt                                                           <  2:184489/81‑1 (MQ=255)           tGCGCAGTAGCGTCCTCGTACAGGAGCGTTTAGTACAATAAACATCTTCAGGAACTGCTGAAGTATACCTTGTTTCGCAAt                                                           <  2:184488/81‑1 (MQ=255)           tGCGCAGTAGCGTCCTCGTACAGGAGCGTTTAGTACAATAAACATCTTCAGGAACTGCTGAAGTATACCTTGTTTCGCAAt                                                           <  2:184487/81‑1 (MQ=255)            gcgcAGTAGCGTCCTCGTACAGGAGCGTTTAGTACAATAAACATCTTCAGGAACTGCTGAAGTATACCTTGTTTCGCAAtt                                                          >  1:184526/1‑81 (MQ=255)              gcAGTAGCGTCCTCGTACAGGAGCGTTTAGTACAATAAACATCTTCAGGAACTGCTGAAGTATACCTTGTTTCGCAATTTa                                                        >  2:184531/1‑81 (MQ=255)                aGTAGCGTCCTCGTACAGGAGCGTTTAGTACAATAAACATCTTCAGGAACTGCTGAAGTATACCTTGTTTCGCAATTTAtt                                                      >  2:184513/1‑81 (MQ=255)                aGTAGCGTCCTCGTACAGGAGCGTTTAGTACAATAAACATCTTCAGGAACTGCTGAAGTATACCTTGTTTCGCAATTTAtt                                                      <  2:184490/81‑1 (MQ=255)                aGTAGCGTCCTCGTACAGGAGCGTTTAGTACAATAAACATCTTCAGGAACTGCTGAAGTATACCTTGTTTCGCAATTTAtt                                                      >  1:184514/1‑81 (MQ=255)                     cGTCCTCGTACAGGAGCGTTTAGTACAATAAACATCTTCAGGAACTGCTGAAGTATACCTTGTTTCGCAATTTATTGAAcc                                                 <  2:184491/81‑1 (MQ=255)                      gTCCTCGTACAGGAGCGTTTAGTACAATAAACATCTTCAGGAACTGCTGAAGTATACCTTGTTTCGCAATTTATTGAAccc                                                >  1:184530/1‑81 (MQ=255)                           cGTACAGGAGCGTTTAGTACAATAAACATCTTCAGGAACTGCTGAAGTATACCTTGTTTCGCAATTTATTGAACCCCGAt                                            >  1:184522/1‑80 (MQ=255)                            gTACAGGAGCGTTTAGTACAATAAACATCTTCAGGAACTGCTGAAGTATACCTTGTTTCGCAATTTATTGAACCCCGATca                                          >  2:184518/1‑81 (MQ=255)                                       tttAGTACAATAAACATCTTCAGGAACTGCTGAAGTATACCTTGTTTCGCAATTTATTGAACCCCGATCACACCATATGcc                               <  2:184492/81‑1 (MQ=255)                                          aGTACAATAAACATCTTCAGGAACTGCTGAAGTATACCTTGTTTCGCAATTTATTGAACCCCGATCACACCATATGCCAcc                            >  2:184532/1‑81 (MQ=255)                                                aTAAACATCTTCAGGAACTGCTGAAGTATACCTTGTTTCGCAATTTATTGAACCCCGATCACACCATATGCCACCTTTCTg                      >  1:184525/1‑81 (MQ=255)                                                aTAAACATCTTCAGGAACTGCTGAAGTATACCTTGTTTCGCAATTTATTGAACCCCGATCACACCATATGCCACCTTTCTg                      >  1:184521/1‑81 (MQ=255)                                                 tAAACATCTTCAGGAACTGCTGAAGTATACCTTGTTTCGCAATTTATTGAACCCCGATCACACCATATGCCACCTTTCTgg                     <  2:184493/81‑1 (MQ=255)                                                   aaCATCTTCAGGAACTGCTGAAGTATACCTTGTTTCGCAATTTATTGAACCCCGATCACACCATATGCCACCTTTCTGGt                    <  2:184494/80‑1 (MQ=255)                                                      aTCTTCAGGAACTGCTGAAGTATACCTTGTTTCGCAATTTATTGAACCCCGATCACACCATATGCCACCTTTCTGGTCGAt                <  1:184496/81‑1 (MQ=255)                                                      aTCTTCAGGAACTGCTGAAGTATACCTTGTTTCGCAATTTATTGAACCCCGATCACACCATATGCCACCTTTCTGGTCGAt                <  1:184495/81‑1 (MQ=255)                                                              gAACTGCTGAAGTATACCTTGTTTCGCAATTTATTGAACCCCGATCACACCATATGCCACCTTTCTGGTCGATGGCATTAc        <  1:184497/81‑1 (MQ=255)                                                                 ctgctgAAGTATACCTTGTTTCGCAATTTATTGAACCCCGATCACACCATATGCCACCTTTCTGGTCGATGGCATTAcgcg     <  1:184498/81‑1 (MQ=255)                                                                    ctgAAGTATACCTTGTTTCGCAATTTATTGAACCCCGATCACACCATATGCCACCTTTCTGGTCGATGGCATTACGCGgtg  <  1:184499/81‑1 (MQ=255)                                                                       |                                                                              GTGTCATAGGTGCGCAGTAGCGTCCTCGTACAGGAGCGTTTAGTACAATAAACATCTTCAGGAACTGCTGGAGTATACCTTGTTTCGCAATTTATTGAACCCCGATCACACCATATGCCACCTTTCTGGTCGATGGCATTACGCGGTG  >  NC_000913/990350‑990497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑