Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	995,400	A→G	W197R (TGG→CGG)	ssuD ←	FMNH2‑dependent alkanesulfonate monooxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	995,400	0	A	G	100.0%	77.5 / NA	23	W197R (TGG→CGG)	ssuD	FMNH2‑dependent alkanesulfonate monooxygenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/9);  total (14/9)

GTCCATGCGCGGCAGCTTTCGCCCGCACTTGTTCGATTTTCTCTTTAACCAGTTCCGGCGGTTCGCCCCAGGTGAGGTAGAGATCAACCTGTTCTGCCGCCAGCTCCTGGGCGACATCTGACGATCCGCCAAAGTAAAGTGGCGGA  >  NC_000913/995331‑995476                                                                      |                                                                             gTCCATGCGCGGCAGCTTTCGCCCGCACTTGTTCGATTTTCTCTTTAACCAGTTCCGGCGGTTCGCCCCGGGTGAGGTa                                                                     >  1:185350/1‑79 (MQ=255)             cAGCTTTCGCCCGCACTTGTTCGATTTTCTCTTTAACCAGTTCCGGCGGTTCGCCCCGGGTGAGGTAGAGATCAACCTGtt                                                       <  2:185317/81‑1 (MQ=255)               gCTTTCGCCCGCACTTGTTCGATTTTCTCTTTAACCAGTTCCGGCGGTTCGCCCCGGGTGAGGTAGAGATCAACCTGTTCt                                                     <  2:185318/81‑1 (MQ=255)                cTTTCGCCCGCACTTGTTCGATTTTCTCTTTAACCAGTTCCGGCGGTTCGCCCCGGGTGAGGTAGAGATCAACCTGTTCTg                                                    <  2:185319/81‑1 (MQ=255)                 tttCGCCCGCACTTGTTCGATTTTCTCTTTAACCAGTTCCGGCGGTTCGCCCCGGGTGAGGTAGAGATCAACCTGTTCTgc                                                   >  1:185347/1‑81 (MQ=255)                  ttCGCCCGCACTTGTTCGATTTTCTCTTTAACCAGTTCCGGCGGTTCGCCCCGGGTGAGGTAGAGATCAACCTGTTCTgcc                                                  >  2:185345/1‑81 (MQ=255)                      cccGCACTTGTTCGATTTTCTCTTTAACCAGTTCCGGCGGTTCGCCCCGGGTGAGGTAGAGATCAACCTGTTCTGCCGCCa                                              <  1:185320/81‑1 (MQ=255)                        cGCACTTGTTCGATTTTCTCTTTAACCAGTTCCGGCGGTTCGCCCCGGGTGAGGTAGAGATCAACCTGTTCTGCCGCCAGc                                            >  2:185370/1‑81 (MQ=255)                        cGCACTTGTTCGATTTTCTCTTTAACCAGTTCCGGCGGTTCGCCCCGGGTGAGGTAGAGATCAACCTGTTCTGCCGCCAGc                                            <  2:185321/81‑1 (MQ=255)                               gTTCGATTTTCTCTTTAACCAGTTCCGGCGGTTCGCCCCGGGTGAGGTAGAGATCAACCTGTTCTGCCGCCAGCTCCTg                                       <  1:185322/79‑1 (MQ=255)                                 tCGATTTTCTCTTTAACCAGTTCCGGCGGTTCGCCCCGGGTGAGGTAGAGATCAACCTGTTCTGCCGCCAGCTCCTGGGc                                    >  1:185367/1‑80 (MQ=255)                                  cGATTTTCTCTTTAACCAGTTCCGGCGGTTCGCCCCGGGTGAGGTAGAGATCAACCTGTTCTGCCGCCAGCTCCTGGGCGa                                  >  2:185352/1‑81 (MQ=255)                                         ctctTTAACCAGTTCCGGCGGTTCGCCCCGGGTGAGGTAGAGATCAACCTGTTCTGCCGCCAGCTCCTGGGCGACATCTGa                           >  2:185349/1‑81 (MQ=255)                                           ctTTAACCAGTTCCGGCGGTTCGCCCCGGGTGAGGTAGAGATCAACCTGTTCTGCCGCCAGCTCCTGGGCGACATCTGAc                          >  2:185344/1‑80 (MQ=255)                                                    gTTCCGGCGGTTCGCCCCGGGTGAGGTAGAGATCAACCTGTTCTGCCGCCAGCTCCTGGGCGACATCTGACGATCCGCCa                 >  2:185353/1‑80 (MQ=255)                                                         ggcggTTCGCCCCGGGTGAGGTAGAGATCAACCTGTTCTGCCGCCAGCTCCTGGGCGACATCTGACGATCCGCCaaagtaa           <  1:185323/81‑1 (MQ=255)                                                            ggTTCGCCCCGGGTGAGGTAGAGATCAACCTGTTCTGCCGCCAGCTCCTGGGCGACATCTGACGATCCGCCaaagtaaagt        >  2:185357/1‑81 (MQ=255)                                                               tCGCCCCGGGTGAGGTAGAGATCAACCTGTTCTGCCGCCAGCTCCTGGGCGACATCTGACGATCCGCCAAAGTAAAGTGGc     >  2:185354/1‑81 (MQ=255)                                                               tCGCCCCGGGTGAGGTAGAGATCAACCTGTTCTGCCGCCAGCTCCTGGGCGACATCTGACGATCCGCCAAAGTAAAGTGGc     >  2:185356/1‑81 (MQ=255)                                                                cGCCCCGGGTGAGGTAGAGATCAACCTGTTCTGCCGCCAGCTCCTGGGCGACATCTGACGATCCGCCAAAGTAAAGTGGc     <  1:185324/80‑1 (MQ=255)                                                                 gCCCCGGGTGAGGTAGAGATCAACCTGTTCTGCCGCCAGCTCCTGGGCGACATCTGACGATCCGCCAAAGTAAAGTGGCgg   <  1:185325/81‑1 (MQ=255)                                                                 gCCCCGGGTGAGGTAGAGATCAACCTGTTCTGCCGCCAGCTCCTGGGCGACATCTGACGATCCGCCAAAGTAAAGTGGCg    >  1:185369/1‑80 (MQ=255)                                                                 gCCCCGGGTGAGGTAGAGATCAACCTGTTCTGCC‑CCAGCTCCTGGGCGACATCTGACGATCCGCCAAAGTAAAGTGGCGGa  >  1:185395/1‑81 (MQ=255)                                                                      |                                                                             GTCCATGCGCGGCAGCTTTCGCCCGCACTTGTTCGATTTTCTCTTTAACCAGTTCCGGCGGTTCGCCCCAGGTGAGGTAGAGATCAACCTGTTCTGCCGCCAGCTCCTGGGCGACATCTGACGATCCGCCAAAGTAAAGTGGCGGA  >  NC_000913/995331‑995476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑