Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	997,898	A→G	T11A (ACT→GCT)	elfA →	putative laminin‑binding fimbrial subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	997,898	0	A	G	100.0%	71.5 / NA	21	T11A (ACT→GCT)	elfA	putative laminin‑binding fimbrial subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/6);  total (15/6)

CATTTAAAGGCATTATCATATTTACGAAGAGATCAGGATGATAACGATGAAAAAAAGTGTATTGACGGCGTTTATAACTGTGGTATGTGCAACGTCCAGCGTTATGGCTGCTGATGATAATGCTATCACGGATGGCTCAGTAACATTTAAT  >  NC_000913/997822‑997972                                                                             |                                                                           cATTTAAAGGCATTATCATATTTACGAAGAGATCAGGATGATAACGATGAAAAAAAGTGTATTGACGGCGTTTATAGCtgt                                                                        >  1:185719/1‑81 (MQ=255)         ggCATTATCATATTTACGAAGAGATCAGGATGATAACGATGAAAAAAAGTGTATTGACGGCGTTTATAGCTGTGGTAtgtg                                                                >  2:185714/1‑81 (MQ=255)             ttATCATATTTACGAAGAGATCAGGATGATAACGATGAAAAAAAGTGTATTGACGGCGTTTATAGCTGTGGTATGTGCAAc                                                            >  1:185712/1‑81 (MQ=255)             ttATCATATTTACGAAGAGATCAGGATGATAACGATGAAAAAAAGTGTATTGACGGCGTTTATAGCTGTGGTATGTGCAAc                                                            >  1:185717/1‑81 (MQ=255)                 cATATTTACGAAGAGATCAGGATGATAACGATGAAAAAAAGTGTATTGACGGCGTTTATAGCTGTGGTATGTGCAACGTc                                                         >  2:185715/1‑80 (MQ=255)                   tatTTACGAAGAGATCAGGATGATAACGATGAAAAAAAGTGTATTGACGGCGTTTATAGCTGTGGTATGTGCAACGTCCAg                                                      >  1:185707/1‑81 (MQ=255)                   tatTTACGAAGAGATCAGGATGATAACGATGAAAAAAAGTGTATTGACGGCGTTTATAGCTGTGGTATGTGCAACGTCCAg                                                      <  2:185689/81‑1 (MQ=255)                    atTTACGAAGAGATCAGGATGATAACGATGAAAAAAAGTGTATTGACGGCGTTTATAGCTGTGGTATGTGCAACGTCCAGc                                                     >  2:185708/1‑81 (MQ=255)                        aagAAGAGATCAGGATGATAACGATGAAAAAAAGTGTATTGACGGCGTTTATAGCTGTGGTATGTGCAACGTCCAGCGtt                                                  <  2:185690/78‑1 (MQ=255)                           aaGAGATCAGGATGATAACGATGAAAAAAAGTGTATTGACGGCGTTTATAGCTGTGGTATGTGCAACGTCCAGCGTTATgg                                              >  1:185699/1‑81 (MQ=255)                                  cAGGATGATAACGATGAAAAAAAGTGTATTGACGGCGTTTATAGCTGTGGTATGTGCAACGTCCAGCGTTATGGCTGCtga                                       >  1:185711/1‑81 (MQ=255)                                   aGGATGATAACGATGAAAAAAAGTGTATTGACGGCGTTTATAGCTGTGGTATGTGCAACGTCCAGCGTTATGGCTGCtgat                                      >  1:185720/1‑81 (MQ=255)                                    ggATGATAACGATGAAAAAAAGTGTATTGACGGCGTTTATAGCTGTGGTATGTGCAACGTCCAGCGTTATGGCTGCtgatg                                     <  2:185691/81‑1 (MQ=255)                                        gatAACGATGAAAAAAAGTGTATTGACGGCGTTTATAGCTGTGGTATGTGCAACGTCCAGCGTTATGGCTGCTGATGATaa                                 >  1:185716/1‑81 (MQ=255)                                           aaCGATGAAAAAAAGTGTATTGACGGCGTTTATAGCTGTGGTATGTGCAACGTCCAGCGTTATGGCTGCTGATGATAATGc                              >  2:185718/1‑81 (MQ=255)                                           aaCGATGAAAAAAAGTGTATTGACGGCGTTTATAGCTGTGGTATGTGCAACGTCCAGCGTTATGGCTGCTGATGATAATGc                              >  1:185722/1‑81 (MQ=255)                                                    aaaaaGTGTATTGACGGCGTTTATAGCTGTGGTATGTGCAACGTCCAGCGTTATGGCTGCTGATGATAATGCTATCACGGa                     <  1:185692/81‑1 (MQ=255)                                                         gtgtATTGACGGCGTTTATAGCTGTGGTATGTGCAACGTCCAGCGTTATGGCTGCTGATGATAATGCTATCACGGATGGCt                <  1:185693/81‑1 (MQ=255)                                                                 aCGGCGTTTATAGCTGTGGTATGTGCAACGTCCAGCGTTATGGCTGCTGATGATAATGCTATCACGGATGGCTCAGTAACa        <  2:185694/81‑1 (MQ=255)                                                                   ggCGTTTATAGCTGTGGTATGTGCAACGTCCAGCGTTATGGCTGCTGATGATAATGCTATCACGGATGGCTCAGTAACAtt      >  1:185703/1‑81 (MQ=255)                                                                       tttATAGCTGTGGTATGTGCAACGTCCAGCGTTATGGCTGCTGATGATAATGCTATCACGGATGGCTCAGTAACATTTAAt  >  1:185738/1‑81 (MQ=255)                                                                             |                                                                           CATTTAAAGGCATTATCATATTTACGAAGAGATCAGGATGATAACGATGAAAAAAAGTGTATTGACGGCGTTTATAACTGTGGTATGTGCAACGTCCAGCGTTATGGCTGCTGATGATAATGCTATCACGGATGGCTCAGTAACATTTAAT  >  NC_000913/997822‑997972

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑