Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	106,233	T→C	V310A (GTT→GCT)	ftsZ →	cell division protein FtsZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	106,233	0	T	C	100.0%	139.2 / NA	40	V310A (GTT→GCT)	ftsZ	cell division protein FtsZ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/19);  total (21/19)

ATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCACTCTGGTGACCAATAAGCAGGTTCAGCAGCCAGT  >  NC_000913/106156‑106308                                                                              |                                                                            aTTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCgctgt                                                                          >  1:23532/1‑81 (MQ=255)     gCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGCTGTTGc                                                                       >  2:23517/1‑80 (MQ=255)     gCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGCTGTTGc                                                                       <  2:23473/80‑1 (MQ=255)     gCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGCTGTTGc                                                                       <  2:23472/80‑1 (MQ=255)      cTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGCTGTTGCg                                                                      <  2:23474/80‑1 (MQ=255)          cGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGCTGTTGCGACAgg                                                                 >  1:23516/1‑81 (MQ=255)           gACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGCTGTTGCGACAGGt                                                                <  1:23475/81‑1 (MQ=255)                   gACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGCTGTTGCGACAGGTATCGGCAt                                                        >  2:23520/1‑81 (MQ=255)                    aCTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGCTGTTGCGACAGGTATCGGCATg                                                       <  2:23476/81‑1 (MQ=255)                     cTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGCTGTTGCGACAGGTATCGGCATgg                                                      <  2:23477/81‑1 (MQ=255)                        tgGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGCTGTTGCGACAGGTATCGGCATGGAc                                                    <  1:23478/80‑1 (MQ=255)                         ggTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGCTGTTGCGACAGGTATCGGCATGGACa                                                   >  1:23529/1‑80 (MQ=255)                              tCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGCTGTTGCGACAGGTATCGGCATGGACAAACGTc                                             <  2:23479/81‑1 (MQ=255)                               cGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGCTGTTGCGACAGGTATCGGCATGGACAAACGTcc                                            >  2:23534/1‑81 (MQ=255)                                ggTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGCTGTTGCGACAGGTATCGGCATGGACAAACGTCCt                                           <  2:23480/81‑1 (MQ=255)                                ggTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGCTGTTGCGACAGGTATCGGCATGGACAAACGTCCt                                           >  2:23518/1‑81 (MQ=255)                                  tACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGCTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGa                                         <  2:23481/81‑1 (MQ=255)                                   aCTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGCTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGa                                         >  1:23528/1‑80 (MQ=255)                                   aCTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGCTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGa                                         >  2:23515/1‑80 (MQ=255)                                       ctctTGACCCGGATATGAATGACGAGCTGCGCGTAACCGCTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCa                                    >  2:23521/1‑81 (MQ=255)                                          ttGACCCGGATATGAATGACGAGCTGCGCGTAACCGCTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCa                                    <  1:23482/78‑1 (MQ=255)                                            gACCCGGATATGAATGACGAGCTGCGCGTAACCGCTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCActct                                <  2:23483/80‑1 (MQ=255)                                            gACCCGGATATGAATGACGAGCTGCGCGTAACCGCTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCACTCTg                               >  2:23527/1‑81 (MQ=255)                                             aCCCGGATATGAATGACGAGCTGCGCGTAACCGCTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCACTCTgg                              >  2:23538/1‑81 (MQ=255)                                             aCCCGGATATGAATGACGAGCTGCGCGTAACCGCTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCACTCTg                               <  1:23484/80‑1 (MQ=255)                                              cccGGATATGAATGACGAGCTGCGCGTAACCGCTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCACTCTGGt                             <  1:23485/81‑1 (MQ=255)                                                 ggATATGAATGACGAGCTGCGCGTAACCGCTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCACTCTGGTGAc                          >  1:23533/1‑81 (MQ=255)                                                 ggATATGAATGACGAGCTGCGCGTAACCGCTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCACTCTGGTGAc                          >  2:23522/1‑81 (MQ=255)                                                     atgaatgaCGAGCTGCGCGTAACCGCTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCACTCTGGTGACCaa                       >  2:23539/1‑80 (MQ=255)                                                          tgaCGAGCTGCGCGTAACCGCTGTTGCGGCAGGTATCGGCATGGACAAACGTCCTGAAATCACTCTGGTGACCAATAAGCa                 <  1:23486/81‑1 (MQ=255)                                                          tgaCGAGCTGCGCGTAACCGCTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCACTCTGGTGACCAATAAg                   >  2:23551/1‑79 (MQ=255)                                                              gAGCTGCGCGTAACCGCTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCACTCTGGTGACCAATAAGCAGGtt             >  2:23530/1‑81 (MQ=255)                                                              gAGCTGCGCGTAACCGCTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCACTCTGGTGACCAATAAGCAGGtt             >  1:23525/1‑81 (MQ=255)                                                              gAGCTGCGCGTAACCGCTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCACTCTGGTGACCAATAAGCAGGtt             >  2:23537/1‑81 (MQ=255)                                                                    cgcgTAACCGCTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCACTCTGGTGACCAATAAGCAGGTTcagcag       <  1:23488/81‑1 (MQ=255)                                                                    cgcgTAACCGCTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCACTCTGGTGACCAATAAGCAGGTTcagcag       >  2:23536/1‑81 (MQ=255)                                                                    cgcgTAACCGCTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCACTCTGGTGACCAATAAGCAGGTTcagc         <  1:23487/79‑1 (MQ=255)                                                                       gTAACCGCTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCACTCTGGTGACCAATAAGCAGGTTCagcagc      >  2:23553/1‑79 (MQ=255)                                                                       gTAACCGCTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCACTCTGGTGACCAATAAGCAGGTTCagcagc      >  1:23549/1‑79 (MQ=255)                                                                        tAACCGCTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCACTCTGGTGACCAATAAGCAGGTTCAGCAGCCAg   <  2:23489/81‑1 (MQ=255)                                                                          aCCGCTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCACTCTGGTGACCAATAAGCAGGTTCAGCAGCCAGt  <  2:23490/80‑1 (MQ=255)                                                                              |                                                                            ATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCACTCTGGTGACCAATAAGCAGGTTCAGCAGCCAGT  >  NC_000913/106156‑106308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑