Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,019,171	T→A	I295F (ATC→TTC)	ompA ←	outer membrane porin A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,019,171	0	T	A	100.0%	81.6 / NA	24	I295F (ATC→TTC)	ompA	outer membrane porin A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (14/10);  total (14/10)

CAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGG  >  NC_000913/1019094‑1019244                                                                              |                                                                          cAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCTAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGAACtt                                                                        >  2:188843/1‑81 (MQ=255)  aGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGAACTTg                                                                       >  1:188848/1‑81 (MQ=255)      cagcaCGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGAACTTGTCTg                                                                   <  1:188817/81‑1 (MQ=255)                tttCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGAACTTGTCTGCCGGGATa                                                           <  1:188818/79‑1 (MQ=255)                    aCGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGAACTTGTCTGCCGGGATACCTTTg                                                     >  1:188855/1‑81 (MQ=255)                          tCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGAACTTGTCTGCCGGGATACCTTTGGAGAt                                                >  1:188851/1‑80 (MQ=255)                          tCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGAACTTGTCTGCCGGGATACCTTTGGAGATc                                               <  1:188819/81‑1 (MQ=255)                               ggTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGAACTTGTCTGCCGGGATACCTTTGGAGATCAGGt                                           >  2:188850/1‑80 (MQ=255)                               ggTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGAACTTGTCTGCCGGGATACCTTTGGAGATCAGGt                                           >  2:188849/1‑80 (MQ=255)                               ggTGTTGCCAGTAACCGGGTTGGATTCGACCATACCACGTGCGGAGAACTTGTCTGCCGGGATACCTTTGGAGATCAGGTa                                          <  2:188820/81‑1 (MQ=255)                                 tgtTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGAACTTGTCTGCCGGGATACCTTTGGAGATCAGGTAAt                                        <  2:188821/81‑1 (MQ=255)                                   ttGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGAACTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATc                                       <  1:188822/80‑1 (MQ=255)                                      ccAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGAACTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATcaac                                    <  2:188823/80‑1 (MQ=255)                                        aGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGAACTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATcaacaa                                  >  2:188860/1‑80 (MQ=255)                                          tAACCGGGTTGGATTCGCCCATACCACGTGCGGAGAACTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAg                               <  1:188824/81‑1 (MQ=255)                                           aaCCGGGTTGGATTCGCCCATACCACGTGCGGAGAACTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGa                              >  2:188856/1‑81 (MQ=255)                                              cGGGTTGGATTCGCCCATACCACGTGCGGAGAACTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACt                            >  2:188859/1‑80 (MQ=255)                                                 gTTGGATTCGCCCATACCACGTGCGGAGAACTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAg                         >  2:188857/1‑80 (MQ=255)                                                     gATTCGCCCATACCACGTGCGGAGAACTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACgg                    >  2:188854/1‑81 (MQ=255)                                                         cGCCCATACCACGTGCGGAGAACTTGTCTGCCGGGATACCTTTGGAGATTAGGTAATCAACAACAGACTGAGCACGGCGCt                >  2:188863/1‑81 (MQ=255)                                                           cccATACCACGTGCGGAGAACTTTTCTTCCTGGATACCTTTTGAGATCATGTAATCAACAACATACTGATCTCCGCGCTCg              >  1:905464/1‑81 (MQ=255)                                                             cATACCACGTGCGGAGAACTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGa            >  1:188861/1‑81 (MQ=255)                                                                  cACGTGCGGAGAACTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGAc       <  1:188825/81‑1 (MQ=255)                                                                        cGGAGAACTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTgg  <  1:188826/80‑1 (MQ=255)                                                                              |                                                                          CAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGTAACCGGGTTGGATTCGCCCATACCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGG  >  NC_000913/1019094‑1019244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑