Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,046,805	T→G	T381P (ACC→CCC)	gfcD ←	putative lipoprotein GfcD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,046,805	0	T	G	100.0%	104.1 / NA	30	T381P (ACC→CCC)	gfcD	putative lipoprotein GfcD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (19/11);  total (19/11)

CAACACGTTGTTGTTGCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCA  >  NC_000913/1046727‑1046882                                                                               |                                                                              cAACACGTTGTTGTTGCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGgtt                                                                             >  2:193313/1‑81 (MQ=255)  aaCACGTTGTTGTTGCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGGTTc                                                                            >  1:193311/1‑81 (MQ=255)         tgttgttgCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGGTTCGGTAGt                                                                      >  1:193320/1‑80 (MQ=255)               tgCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGGTTCGGTAGTCACCATc                                                               >  2:193315/1‑81 (MQ=255)                gCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGGTTCGGTAGTCACCATc                                                               <  1:193271/80‑1 (MQ=255)                            aTTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGGTTCGGTAGTCACCATCGCCATATGCt                                                     <  2:193273/78‑1 (MQ=255)                            aTTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGACGGGTTCGGTAGTCACCATCGCCATATGCTcgc                                                  <  2:193272/81‑1 (MQ=255)                                  cAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGGTTCGGTAGTCACCATCGCCATATGCTCGCGCtttt                                            <  2:193274/81‑1 (MQ=255)                                       gcTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGGTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGt                                        >  2:193316/1‑80 (MQ=255)                                       gcTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGGTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGt                                        >  1:193331/1‑80 (MQ=255)                                           tACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGGTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCAc                                     <  1:193275/79‑1 (MQ=255)                                           tACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGGTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGc                                   <  2:193276/81‑1 (MQ=255)                                            aCCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGGTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGc                                   >  1:193323/1‑80 (MQ=255)                                             ccTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGGTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCt                                  <  2:193277/80‑1 (MQ=255)                                                gCCAGCTGTTTGCGCAGGCTGGCTACGTCGGGTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAAtc                              >  1:193314/1‑81 (MQ=255)                                                gCCAGCTGTTTGCGCAGGCTGGCTACGTCGGGTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAAtc                              >  2:193327/1‑81 (MQ=255)                                                gCCAGCTGTTTGCGCAGGCTGGCTACGTCGGGTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAAtc                              >  2:193321/1‑81 (MQ=255)                                                     cTGTTTGCGCAGGCTGGCTACGTCGGGTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTtctc                         <  2:193278/81‑1 (MQ=255)                                                           gcgcAGGCTGGCTACGTCGGGTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGcc                   >  1:193318/1‑81 (MQ=255)                                                           gcgcAGGCTGGCTACGTCGGGTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGcc                   >  2:193324/1‑81 (MQ=255)                                                             gcAGGCTGGCTACGTCGGGTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCtt                 >  2:193341/1‑81 (MQ=255)                                                             gcAGGCTGGCTACGTCGGGTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCtt                 <  2:193279/81‑1 (MQ=255)                                                              cAGGCTGGCTACGTCGGGTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTg                >  2:193333/1‑81 (MQ=255)                                                                    ggctACGTCGGGTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAg          >  2:193322/1‑81 (MQ=255)                                                                      ctACGTCGGGTTAGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGt        >  2:193336/1‑81 (MQ=255)                                                                       tACGTCGGGTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGt        >  2:193328/1‑80 (MQ=255)                                                                         cGTCGGGTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGtt       <  1:193280/79‑1 (MQ=255)                                                                         cGTCGGGTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTAt     >  1:193312/1‑81 (MQ=255)                                                                          gTCGGGTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTAtt    >  1:193329/1‑81 (MQ=255)                                                                           tCGGGTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTc   <  1:193281/81‑1 (MQ=255)                                                                            cGGGTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCa  >  1:193330/1‑81 (MQ=255)                                                                               |                                                                              CAACACGTTGTTGTTGCAGTGGCTCTGATTGACCAGGCGCTGTACCTGCCAGCTGTTTGCGCAGGCTGGCTACGTCGGTTTCGGTAGTCACCATCGCCATATGCTCGCGCTTTTGCGTCACGCTAATCTTCTCAACGCCTTGCGGCAGGTTATTCA  >  NC_000913/1046727‑1046882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑