Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,064,853	A→T	D273V (GAT→GTT)	yccE →	uncharacterized protein YccE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,064,853	0	A	T	100.0%	47.4 / NA	14	D273V (GAT→GTT)	yccE	uncharacterized protein YccE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (5/9);  total (5/9)

AAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGACAACCAAATTCAGGGGCAGAGTAGTCTTATGTCATGGAGCTTTCTGTTTTTTAAAA  >  NC_000913/1064778‑1064921                                                                            |                                                                     aaaaaaCGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGTTCTa                                                                  >  1:196038/1‑80 (MQ=255)  aaaaaCGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGTTCTACa                                                                <  2:196019/81‑1 (MQ=255)    aaaCGATCGAGGCTCTTGTTGATACTGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGTTCTACAAt                                                              >  2:196035/1‑81 (MQ=255)        gATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGTTCTACAATCTGa                                                          <  2:196020/81‑1 (MQ=255)                            cGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGTTCTACAATCTGACAACCAAATTCAGGGGCag                                       <  1:196022/80‑1 (MQ=255)                                aTTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGTTCTACAATCTGACAACCAAATTCAGGGGCAGagtag                                  >  1:196042/1‑81 (MQ=255)                                        aTCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGTTCTACAATCTGACAACCAAATTCAGGGGCAGAGTAGTCTTATg                           <  1:196023/80‑1 (MQ=255)                                             aGGGAAGATGTAAAGACGTTATTTGGCGTTGTTCTACAATCTGACAACCAAATTCAGGGGCAGAGTAGTCTTATGTCAt                       <  1:196024/79‑1 (MQ=255)                                                gAAGATGTAAAGACGTTATTTGGCGTTGTTCTACAATCTGACAACCAAATTCAGGGGCAGAGTAGTCTTATGTCATGGAGc                  <  1:196025/81‑1 (MQ=255)                                                  aGATGTAAAGACGTTATTTGGCGTTGTTCTACAATCTGACAACCAAATTCAGGGGCAGAGTAGTCTTATGTCATGGAGCtt                <  2:196026/81‑1 (MQ=255)                                                    aTGTAAAGACGTTATTTGGCGTTGTTCTACAATCTGACAACCAAATTCAGGGGCAGAGTAGTCTTATGTCATGGAGCTTTc              <  2:196027/81‑1 (MQ=255)                                                              gTTATTTGGCGTTGTTCTACAATCTGACAACCAAATTCAGGGGCAGAGTAGTCTTATGTCATGGAGCTTTCTGTTTTTTa     >  1:196039/1‑80 (MQ=255)                                                              gTTATTTGGCGTTGTTCTACAATCTGACAACCAAATTCAGGGGCAGAGTAGTCTTATGTCATGGAGCTTTCTGTTTTTTa     >  1:196041/1‑80 (MQ=255)                                                                 aTTTGGCGTTGTTCTACAATCTGACAACCAAATTCAGGGGCAGAGTAGTCTTATGTCATGGAGCTTTCTGTTTTTTaaaa  <  1:196028/80‑1 (MQ=255)                                                                            |                                                                     AAAAAACGATCGAGGCTCTTGTTGATACGGCATTCTATAATCTCAGGGAAGATGTAAAGACGTTATTTGGCGTTGATCTACAATCTGACAACCAAATTCAGGGGCAGAGTAGTCTTATGTCATGGAGCTTTCTGTTTTTTAAAA  >  NC_000913/1064778‑1064921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑