Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,065,984	A→G	I134V (ATT→GTT)	agp →	glucose‑1‑phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,065,984	0	A	G	100.0%	86.6 / NA	26	I134V (ATT→GTT)	agp	glucose‑1‑phosphatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/20);  total (6/20)

TGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAACCCGGTGATCACCGATGATTCCGCCG  >  NC_000913/1065914‑1066059                                                                       |                                                                            tgccAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATGTTCCtgtg                                                                     <  2:196154/79‑1 (MQ=255) tgccAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATGTTCCTGTGc                                                                    <  2:196156/80‑1 (MQ=255) tgccAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATGTTCCTGTGc                                                                    <  2:196155/80‑1 (MQ=255)   ccAATTGTCTGCAACGTACCGTTGCGACCGCACAGTCCTTTATTACCGGCGCATTCCCGGGGTGTGATGTTCCTGTGCATc                                                                 <  1:196158/81‑1 (MQ=255)   ccAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATGTTCCTGTGCATc                                                                 <  1:196157/81‑1 (MQ=255)              cAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATGTTCCTGTGCATCACCAGGaa                                                         <  1:196159/78‑1 (MQ=255)                  gTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATGTTCCTGTGCATCACCAGGAAAAAATgg                                                  >  1:196191/1‑81 (MQ=255)                       gTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATGTTCCTGTGCATCACCAGGAAAAAATGGGCACt                                             <  2:196160/81‑1 (MQ=255)                       gTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATGCTCCTGTGCATCACCAGGAAAAAATGGGCAc                                              >  2:196195/1‑80 (MQ=255)                         tGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATGTTCCTGTGCATCACCAGGAAAAAATGGGCACTAt                                           >  2:196193/1‑81 (MQ=255)                           cGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATGTTCCTGTGCATCACCAGGAAAAAATGGGCACTATgg                                         <  1:196161/81‑1 (MQ=255)                               cGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATGTTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGAcc                                      >  1:196202/1‑80 (MQ=255)                                  acaGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATGTTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAAc                                  >  2:196192/1‑81 (MQ=255)                                   caGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATGTTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAAcc                                 <  2:196162/81‑1 (MQ=255)                                         tttATTACCGGCGCATTCCCGGGGTGTGATGTTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAAc                           >  1:196204/1‑81 (MQ=255)                                           tattaCCGGCGCATTCCCGGGGTGTGATGTTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAAc                           <  2:196163/79‑1 (MQ=255)                                                   gcgcATTCCCGGGGTGTGATGTTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAACCCGGTGATCa                 <  1:196164/81‑1 (MQ=255)                                                    cgcATTCCCGGGGTGTGATGTTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAACCCGGTGATCAc                <  1:196165/81‑1 (MQ=255)                                                        ttCCCGGGGTGTGATGTTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAACCCGGTGATCACCgat            <  1:196166/81‑1 (MQ=255)                                                        ttCCCGGGGTGTGATGTTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAACCCGGTGATCACCgat            <  1:196167/81‑1 (MQ=255)                                                          cccGGGGTGTGATGTTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAACCCGGTGATCACCgatg           <  1:196168/80‑1 (MQ=255)                                                           ccGGGGTGTGATGTTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAACCCGGTGATCACCgatga          <  2:196169/80‑1 (MQ=255)                                                            cGGGGTGTGATGTTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAACCCGGTGATCACCGATGAtt        <  1:196170/81‑1 (MQ=255)                                                              gggTGTGATGTTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAACCCGGTGATCACCGATGATTcc      <  1:196171/81‑1 (MQ=255)                                                                gtgtGATGTTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAACCCGGTGATCACCGATGATTccgc    <  2:196172/81‑1 (MQ=255)                                                                   tgATGTTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAACCCGGTGATCACCGATGATTccgccg  <  1:196173/80‑1 (MQ=255)                                                                       |                                                                            TGCCAATAGTCTGCAACGTACCGTTGCGACCGCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAAAAAATGGGCACTATGGACCCAACCTTTAACCCGGTGATCACCGATGATTCCGCCG  >  NC_000913/1065914‑1066059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑