Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,068,977	C→G	G288A (GGC→GCC)	rutG ←	pyrimidine:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,068,977	0	C	G	100.0%	62.7 / NA	19	G288A (GGC→GCC)	rutG	pyrimidine:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (8/11);  total (8/11)

CCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGCCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGCCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGTCATCCCGGCGACTGCCTTGAGATGACCTAA  >  NC_000913/1068905‑1069050                                                                         |                                                                          ccGATATTTTCAGCATAGGTGGTCACGCCGCTACCGCCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGGCTACGAAt                                                                   >  2:196618/1‑81 (MQ=255)  cGATATTTTCAGCATAGGTGGTCACGCCGCTACCGCCGACAGAGCCGGAAAGCATCGTTGCCAGACCGTCGGCTACGAATg                                                                  <  2:196592/81‑1 (MQ=255)  cGATATTTTCAGCATAGGTGGTCACGCCGCTACCGCCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGGCTACGAATg                                                                  <  2:196593/81‑1 (MQ=255)       ttttCAGCATAGGTGGTCACGCCGCTACCGCCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGGCTACGAATgcccgc                                                             <  2:196594/81‑1 (MQ=255)           cAGCATAGGTGGTCACGCCGCTACCGCCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGGCTACGAATGCCCGcccc                                                          <  1:196595/80‑1 (MQ=255)             gCATAGGTGGTCACGCCGCTACCGCCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGGCTACGAATGCCCGCCCCata                                                       >  1:196635/1‑81 (MQ=255)               aTAGGTGGTCACGCCGCTACCGCCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGGCTACGAATGCCCGCCCCAtata                                                     >  2:196623/1‑81 (MQ=255)               aTAGGTGGTCACGCCGCTACCGCCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGGCTACGAATGCCCGCCCCAtata                                                     >  1:196630/1‑81 (MQ=255)                           gccgcTACCGCCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGGCTACGAATGCCCGCCCCATATACGGGTCCATAtt                                         >  1:196627/1‑81 (MQ=255)                                 aCCGCCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGGCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTcc                                   >  2:196642/1‑81 (MQ=255)                                  ccgccgACAGAGCCGGAAAGCATCGTTGCCAGACCATCGGCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTcc                                   <  2:196596/80‑1 (MQ=255)                                     ccgACAGAGCCGGAAAGCATCGTTGCCAGACCATCGGCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGt                                >  1:196631/1‑80 (MQ=255)                                       gACAGAGCCGGAAAGCATCGTTGCCAGACCATCGGCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGTCAt                             <  1:196598/81‑1 (MQ=255)                                       gACAGAGCCGGAAAGCATCGTTGCCAGACCATCGGCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGTCAt                             <  1:196597/81‑1 (MQ=255)                                            agCCGGAAAGCATCGTTGCCAGACCATCGGCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGTCATCCCgg                        >  2:196634/1‑81 (MQ=255)                                                      cATCGTTGCCAGACCATCGGCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGTCATCCCGGCGACTGCCtt              <  2:196599/81‑1 (MQ=255)                                                      cATCGTTGCCAGACCATCGGCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGTCATCCCGGCGACTGCCtt              <  2:196600/81‑1 (MQ=255)                                                          gTTGCCAGACCATCGGCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGTCATCCCGGCGACTGCCTTgag           <  2:196601/80‑1 (MQ=255)                                                                  aCCATCGGCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGTCATCCCGGCGACTGCCTTGAGATGACCTaa  <  2:196602/81‑1 (MQ=255)                                                                         |                                                                          CCGATATTTTCAGCATAGGTGGTCACGCCGCTACCGCCGACAGAGCCGGAAAGCATCGTTGCCAGACCATCGCCTACGAATGCCCGCCCCATATACGGGTCCATATTGCGTCCGGTCATCCCGGCGACTGCCTTGAGATGACCTAA  >  NC_000913/1068905‑1069050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑