Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	109,267	A→G	D330G (GAT→GGT)	secA →	protein translocation ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	109,267	0	A	G	100.0%	119.4 / NA	35	D330G (GAT→GGT)	secA	protein translocation ATPase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/17);  total (18/17)

TCATGCTGATGCACCACGTAACGGCGGCGCTGCGCGCTCATGCGCTGTTTACCCGTGACGTCGACTACATCGTTAAAGATGGTGAAGTTATCATCGTTGACGAACACACCGGTCGTACCATGCAGGGCCGTCGCTGGTCCGATGGTCTGCACCAG  >  NC_000913/109189‑109343                                                                               |                                                                             tcatGCTGATGCACCACGTAACGGCGGCGCTGCGCGCTCATGCGCTGTTTACCCGTGACGTCGACTACATCGTTAAAGGTg                                                                            >  1:24150/1‑81 (MQ=255)   atGCTGATGCACCACGTAACGGCGGCGCTGCGCGCTCATGCGCTGTTTACCCGTGACGTCGACTACATCGTTAAAggtggt                                                                          <  1:24105/81‑1 (MQ=255)           gCACCACGTAACGGCGGCGCTGCGCGCTCATGCGCTGTTTACCCGTGACGTCGACTACATCGTTAAAGGTGGTGAAGTTat                                                                  >  2:24155/1‑81 (MQ=255)                 cGTAACGGCGGCGCTGCGCGCTCATGCGCTGTTTACCCGTGACGTCGACTACATCGTTAAAGGTGGTGAAGTTATCATCGt                                                            >  2:24152/1‑81 (MQ=255)                  gTAACGGCGGCGCTGCGCGCTCATGCGCTGTTTACCCGTGACGTCGACTACATCGTTAAAGGTGGTGAAGTTATCATCGtt                                                           >  2:24164/1‑81 (MQ=255)                   tAACGGCGGCGCTGCGCGCTCATGCGCTGTTTACCCGTGACGTCGACTACATCGTTAAAGGTGGTGAAGTTATCATCGTTg                                                          <  2:24106/81‑1 (MQ=255)                     aCGGCGGCGCTGCGCGCTCATGCGCTGTTTACCCGTGACGTCGACTACATCGTTAAAGGTGGTGAAGTTATCATCGTTGAc                                                        <  1:24107/81‑1 (MQ=255)                       ggcggcGCTGCGCGCTCATGCGCTGTTTACCCGTGACGTCGACTACATCGTTAAAGGTGGTGAAGTTATCATCGTTGACGa                                                      <  2:24108/81‑1 (MQ=255)                           gcgcTGCGCGCTCATGCGCTGTTTACCCGTGACGTCGACTACATCGTTAAAGGTGGTGAAGTTATCATCGTTGACGAacac                                                  <  1:24109/81‑1 (MQ=255)                             gcTGCGCGCTCATGCGCTGTTTACCCGTGACGTCGACTACATCGTTAAAGGTGGTGAAGTTATCATCGTTGACGAacacac                                                >  2:24176/1‑81 (MQ=255)                             gcTGCGCGCTCATGCGCTGTTTACCCGTGACGTCGACTACATCGTTAAAGGTGGTGAAGTTATCATCGTTGACGAacacac                                                >  2:24167/1‑81 (MQ=255)                                 cgcgcTCATGCGCTGTTTACCCATGACGTCGACTACATCGTTAAAGGTGGTGAAGTTATCATCGTTGACGAACACACCGGt                                            <  1:651224/81‑1 (MQ=255)                                    gcTCATGCGCTGTTTACCCGTGACGTCGACTACATCGTTAAAGGTGGTGAAGTTATCATCGTTGACGAACACACCGGTCg                                          >  2:24160/1‑80 (MQ=255)                                    gcTCATGCGCTGTTTACCCGTGACGTCGACTACATCGTTAAAGGTGGTGAAGTTATCATCGTTGACGAACACACCGGTCGt                                         <  2:24111/81‑1 (MQ=255)                                    gcTCATGCGCTGTTTACCCGTGACGTCGACTACATCGTTAAAGGTGGTGAAGTTATCATCGTTGACGAACACACCGGTCGt                                         <  1:24110/81‑1 (MQ=255)                                                  tACCCGTGACGTCGACTACATCGTTAAAGGTGGTGAAGTTATCATCGTTGACGAACACACCGGTCGTACCATGCAGGGcc                            <  2:24112/80‑1 (MQ=255)                                                  tACCCGTGACGTCGACTACATCGTTAAAGGTGGTGAAGTTATCATCGTTGACGAACACACCGGTCGTACCATGCAGGGCCg                           >  1:24175/1‑81 (MQ=255)                                                    cccGTGACGTCGACTACATCGTTAAAGGTGGTGAAGTTATCATCGTTGACGAACACACCGGTCGTACCATGCAGGGCCGTc                         <  1:24115/81‑1 (MQ=255)                                                    cccGTGACGTCGACTACATCGTTAAAGGTGGTGAAGTTATCATCGTTGACGAACACACCGGTCGTACCATGCAGGGCCGTc                         <  2:24113/81‑1 (MQ=255)                                                    cccGTGACGTCGACTACATCGTTAAAGGTGGTGAAGTTATCATCGTTGACGAACACACCGGTCGTACCATGCAGGGCCGTc                         <  2:24114/81‑1 (MQ=255)                                                      cGTGACGTCGACTACATCGTTAAAGGTGGTGAAGTTATCATCGTTGACGAACACACCGGTCGTACCATGCAGGGCCGTCGc                       >  2:24159/1‑81 (MQ=255)                                                       gTGACGTCGACTACATCGTTAAAGGTGGTGAGGTTATCATCGTTGACGAACACACCGGTCGTACCATGCAGGGCCGTCGCt                      <  2:24116/81‑1 (MQ=255)                                                       gTGACGTCGACTACATCGTTAAAGGTGGTGAAGTTATCATCGTTGACGAACACACCGGTCGTACCATGCAGGGCCGTCGCt                      >  2:24161/1‑81 (MQ=255)                                                       gTGACGTCGACTACATCGTTAAAGGTGGTGAAGTTATCATCGTTGACGAACACACCGGTCGTACCATGCAGGGCCGTCGCt                      >  1:24165/1‑81 (MQ=255)                                                       gTGACGTCGACTACATCGTTAAAGGTGGTGAAGTTATCATCGTTGACGAACACACCGGTCGTACCATGCAGGGCCGTCGCt                      <  1:24117/81‑1 (MQ=255)                                                        tGACGTCGACTACATCGTTAAAGGTGGTGAAGTTATCATCGTTGACGAACACACCGGTCGTACCATGCAGGGCCGTCGCTg                     >  1:24162/1‑81 (MQ=255)                                                          aCGTCGACTACATCGTTAAAGGTGGTGAAGTTATCATCGTTGACGAACATACCGGTCGTACCATGCAGGGCCGTCGCTGGt                   >  2:24171/1‑81 (MQ=255)                                                          aCGTCGACTACATCGTTAAAGGTGGTGAAGTTATCATCGTTGACGAACACACCGGTCGTACCATGCAGGGCCGTCGCTGGt                   >  2:24169/1‑81 (MQ=255)                                                            gTCGACTACATCGTTAAAGGTGGTGAAGTTATCATCGTTGACGAACACACCGGTCGTACCATGCAGGGCCGTCGCTGGTcc                 >  2:24168/1‑81 (MQ=255)                                                              cGACTACATCGTTAAAGGTGGTGAAGTTATCATCGTTGACGAACACACCGGTCGTACCATGCATGGCCGTCGCTGGTCCGa               >  1:24180/1‑81 (MQ=255)                                                               gACTACATCGTTAAAGGTGGTGAAGTTATCATCGTTGACGAACACACCGGTCGTACCATGCAGGGCCGTCGCTGGTCCGa               <  1:24118/80‑1 (MQ=255)                                                               gACTACATCGTTAAAGGTGGTGAAGTTATCATCGTTGACGAACACACCGGTCGTACCATGCAGGGCCGTCGCTGGTCCGa               <  2:24119/80‑1 (MQ=255)                                                                aCTACATCGTTAAAGGTGGTGAAGTTATCATCGTTGACGAACACACCGGTCGTACCATGCAGGGCCGTCGCTGGTCCGATg             <  1:24120/81‑1 (MQ=255)                                                                       cGTTAAAGGTGGTGAAGTTATCATCGTTGACGAACACACCGGTCGTACCATGCAGGGCCGTCGCTGGTCCGATGGTCTGCa      >  2:24174/1‑81 (MQ=255)                                                                        gTTAAAGGTGGTGAAGTTATCATCGTTGACGAACACACCGGTCGTACCATGCAGGGCCGTCGCTGGTCCGATGGTCTGCAc     >  2:24163/1‑81 (MQ=255)                                                                            aaGGTGGTGAAGTTATCATCGTTGACGAACACACCGGTCGTACCATGCAGGGCCGTCGCTGGTCCGATGGTCTGCACCAg  <  2:24121/80‑1 (MQ=255)                                                                               |                                                                             TCATGCTGATGCACCACGTAACGGCGGCGCTGCGCGCTCATGCGCTGTTTACCCGTGACGTCGACTACATCGTTAAAGATGGTGAAGTTATCATCGTTGACGAACACACCGGTCGTACCATGCAGGGCCGTCGCTGGTCCGATGGTCTGCACCAG  >  NC_000913/109189‑109343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑