Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,100,298	G→A	M1M (ATG→ATA) †	ycdZ →	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,100,298	0	G	A	100.0%	69.9 / NA	20	M1M (ATG→ATA) †	ycdZ	putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/7);  total (13/7)

AACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTGGGGATGGGTGGCTGTT  >  NC_000913/1100221‑1100364                                                                              |                                                                   aaCGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATAAAc                                                                 <  1:201100/81‑1 (MQ=255) aaCGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATAAAc                                                                 <  2:201099/81‑1 (MQ=255)    ggATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATAAACATa                                                              >  1:201123/1‑81 (MQ=255)              ttgttgCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATAAACATACTTCTCTCTa                                                    <  2:201101/81‑1 (MQ=255)                gttgCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATAAACATACTTCTCTCTAtt                                                  >  2:201121/1‑81 (MQ=255)                         aaTGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATAAACATACTTCTCTCTATTGCAATcac                                          >  1:201116/1‑80 (MQ=255)                               tgCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATAAACATACTTCTCTCTATTGCAATCACAACAgg                                    <  1:201102/80‑1 (MQ=255)                                    gATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATAAACATACTTCTCTCTATTGCAATCACAACAGGCAtt                                <  1:201105/79‑1 (MQ=255)                                    gATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATAAACATACTTCTCTCTATTGCAATCACAACAGGCATtc                               <  1:201103/80‑1 (MQ=255)                                    gATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATAAACATACTTCTCTCTATTGCAATCACAACAGGCATtc                               <  1:201104/80‑1 (MQ=255)                                     aTCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATAAACATACTTCTCTCTATTGCAATCACAACAGGCATtctc                             >  2:201128/1‑81 (MQ=255)                                          attcatGGCCGCGTTTTCTGCTATCATGCGCGGCATAAACATACTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCgg                        >  2:201134/1‑81 (MQ=255)                                          attcatGGCCGCGTTTTCTGCTATCATGCGCGGCATAAACATACTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCgg                        >  2:201131/1‑81 (MQ=255)                                            tcatGGCCGCGTTTTCTGCTATCATGCGCGGCATAAACATACTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTa                      >  2:201137/1‑81 (MQ=255)                                                          tCTGCTATCATGCGCGGCATAAACATACTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTGGGGATGGGTg        >  2:201120/1‑81 (MQ=255)                                                          tCTGCTATCATGCGCGGCATAAACATACTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTGGGGATGGGTg        >  1:201127/1‑81 (MQ=255)                                                              cTATCATGCGCGGCATAAACATACTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTGGGGATGGGTGGCTg    >  1:201135/1‑81 (MQ=255)                                                               tATCATGCGCGGCATAAACATACTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTGGGGATGGGTGGCTGt   >  1:201122/1‑81 (MQ=255)                                                                aTCATGCGCGGCATAAACATACTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTGGGGATGGGTGGCTGtt  >  2:201126/1‑81 (MQ=255)                                                                aTCATGCGCGGCATAAACATACTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTGGGGATGGGTGGCTGtt  >  1:201129/1‑81 (MQ=255)                                                                              |                                                                   AACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTGGGGATGGGTGGCTGTT  >  NC_000913/1100221‑1100364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑