Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,104,203	A→G	T85A (ACA→GCA)	csgB →	curlin, minor subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,104,203	0	A	G	100.0%	44.2 / NA	14	T85A (ACA→GCA)	csgB	curlin, minor subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/5);  total (9/5)

CAGGGAGGCTCAAAACTTTTGGCGGTTGTTGCGCAAGAAGGTAGTAGCAACCGGGCAAAGATTGACCAGACAGGAGATTATAACCTTGCATATATTGATCAGGCGGGCAGTGCCAACGATGCCAGTATTTCGCAAGGTGCT  >  NC_000913/1104134‑1104274                                                                      |                                                                        cAGGGAGGCTCAAAACTTTTGGCGGTTGTTGCGCAAGAAGGTAGTAGCAACCGGGCAAAGATTGACCAGGCAGGAGATta                                                               >  1:201595/1‑80 (MQ=255)   gggAGGCTCAAAACTTTTGGCGGTTGTTGCGCAAGAAGGTAGTAGCAACCGGGCAAAGATTGACCAGGCAGGAGATTATaa                                                            <  2:201570/81‑1 (MQ=255)      aGGCTCAAAACTTTTGGCGGTTGTTGCGCAAGAAGGTAGTAGCAACCGGGCAAAGATTGACCAGGCAGGAGATTATAACCt                                                         >  2:201589/1‑81 (MQ=255)        gCTCAAAACTTTTGGCGGTTGTTGCGCAAGAAGGTAGTAGCAACCGGGCAAAGATTGACCAGGCAGGAGATTATAACCTTg                                                       <  2:201571/81‑1 (MQ=255)           cAAAACTTTTGGCGGTTGTTGCGCAAGAAGGTAGTAGCAACCGGGCAAAGATTGACCAGGCAGGAGATTATAACCTTGCa                                                     >  2:201588/1‑80 (MQ=255)             aaaCTTTTGGCGGTTGTTGCGCAAGAAGGTAGTAGCGACCGGGCAAAGATTGACCAGGCAGGAGATTATAACCTTGCata                                                   >  1:201594/1‑80 (MQ=255)                   ttGGCGGTTGTTGCGCAAGAAGGTAGTAGCAACCGGGCAAAGATTGACCAGGCAGGAGATTATAACCTTGCATATATTGAt                                            >  2:201587/1‑81 (MQ=255)                               gcgcAAGAAGGTAGTAGCAACCGGGCAAAGATTGACCAGGCAGGAGATTATAACCTTGCATATATTGATCAGGCGGGCAg                                 <  2:457734/80‑1 (MQ=255)                                   aagaagGTAGTAGCAACCGGGCAAAGATTGACCAGGCAGGAGATTATAACCTTGCATATATTGATCAGGCGGGCAGTGCCa                            <  1:201572/81‑1 (MQ=255)                                            gtagCAACCGGGCAAAGATTGACCAGGCAGGAGATTATAACCTTGCATATATTGATCAGGCGGGCAGTGCCAACGATGcc                    <  1:201573/80‑1 (MQ=255)                                              agCAACCGGGCAAAGATTGACCAGGCAGGAGATTATAACCTTGCATATATTGATCAGGCGGGCAGTGCCAACGATGCCAg                  >  1:201597/1‑80 (MQ=255)                                                     gggCAAAGATTGACCAGGCAGGAGATTATAACCTTGCATATATTGATCAGGCGGGCAGTGCCAACGATGCCAGTATTTCg           >  1:201608/1‑80 (MQ=255)                                                       gCAAAGATTGACCAGGCAGGAGATTATAACCTTGCATATATTGATCAGGCGGGCAGTGCCAACGATGCCAGTATTTCGc          >  1:201596/1‑79 (MQ=255)                                                             aTTGACCAGGCAGGAGATTATAACCTTGCATATATTGATCAGGCGGGCAGTGCCAACGATGCCAGTATTTCGCAAGGTGCt  >  1:201606/1‑81 (MQ=255)                                                                      |                                                                        CAGGGAGGCTCAAAACTTTTGGCGGTTGTTGCGCAAGAAGGTAGTAGCAACCGGGCAAAGATTGACCAGACAGGAGATTATAACCTTGCATATATTGATCAGGCGGGCAGTGCCAACGATGCCAGTATTTCGCAAGGTGCT  >  NC_000913/1104134‑1104274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑