Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,114,355	A→G	V379A (GTA→GCA)	mdtG ←	efflux pump MdtG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,114,355	0	A	G	100.0%	74.2 / NA	22	V379A (GTA→GCA)	mdtG	efflux pump MdtG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/13);  total (9/13)

TATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGCTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGAT  >  NC_000913/1114282‑1114432                                                                          |                                                                              tATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATGCCGctct                                                                        <  1:202995/81‑1 (MQ=255)       acgacgacgTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATGCCGCTCTGAAAcc                                                                  <  1:202996/81‑1 (MQ=255)           cgacgTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATGCCGCTCTGAAACCGTAg                                                              <  1:202997/81‑1 (MQ=255)              cgTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATGCCGCTCTGAAACCGTAGTTc                                                           >  1:203032/1‑81 (MQ=255)                tAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATGCCGCTCTGAAACCGTAGTTCGc                                                         <  1:202998/81‑1 (MQ=255)                tAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATGCCGCTCTGAAACCGTAGTTCGc                                                         >  2:203033/1‑81 (MQ=255)                 aGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATGCCGCTCTGAAACCGTAGTTCGCt                                                        >  2:203030/1‑81 (MQ=255)                 aGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATGCCGCTCTGAAACCGTAGTTCGCt                                                        >  1:203029/1‑81 (MQ=255)                  gACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATGCCGCTCTGAAACCGTAGTTCGCt                                                        <  2:202999/80‑1 (MQ=255)                        tCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATGCCGCTCTGAAACCGTAGTTCGCTGAAATCg                                                 >  1:203034/1‑81 (MQ=255)                            tGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATGCCGCTCTGAAACCGTAGTTCGCTGAAATCgctgc                                             <  1:203000/81‑1 (MQ=255)                                tAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATGCCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGCTccc                                         <  1:203001/81‑1 (MQ=255)                                             aTAACACTACGCCAGCGGTGACGAGAAATGCCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGCTCCCATCAATGGTCCgg                            >  1:203035/1‑81 (MQ=255)                                              tAACACTACGCCAGCGGTGACGAGAAATGCCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGCTCCCATCAATGGTCCGGt                           <  1:203003/81‑1 (MQ=255)                                              tAACACTACGCCAGCGGTGACGAGAAATGCCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGCTCCCATCAATGGTCCGGt                           <  1:203002/81‑1 (MQ=255)                                                       gCCAGCGGTGACGAGAAATGCCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGCTCCCATCAATGGACCGGTAACGTTGc                   >  2:203039/1‑80 (MQ=255)                                                        ccAGCGGTGACGAGAAATGCCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGCTCCCATCAATGGTCCGGTAACGTTGCCa                 <  2:203004/81‑1 (MQ=255)                                                           gCGGTGACGAGAAATGCCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGCTCCCATCAATGGTCCGGTAACGTTGCCAata              >  1:203047/1‑81 (MQ=255)                                                            cGGTGACGAGAAATGCCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGCTCCCATCAATGGTCCGGTAACGTTGCCAatat             <  2:203005/81‑1 (MQ=255)                                                                   gagaAATGCCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGCTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGaaa      >  1:203044/1‑81 (MQ=255)                                                                    agaAATGCCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGCTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAAc     <  2:203006/81‑1 (MQ=255)                                                                       aaTGCCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGCTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGAt  <  1:203007/81‑1 (MQ=255)                                                                          |                                                                              TATTCGACGACGACGTAGACTGTTCCATGAATAGACTGCGTTGAATAACACTACGCCAGCGGTGACGAGAAATACCGCTCTGAAACCGTAGTTCGCTGAAATCGCTGCTCCCATCAATGGTCCGGTAACGTTGCCAATATCACGAAACGAT  >  NC_000913/1114282‑1114432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑