Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,128,283	G→T	A149S (GCG→TCG)	murJ →	putative lipid II flippase MurJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,128,283	0	G	T	100.0%	83.1 / NA	24	A149S (GCG→TCG)	murJ	putative lipid II flippase MurJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (17/7);  total (17/7)

AATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTC  >  NC_000913/1128206‑1128360                                                                              |                                                                              aaTTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGATCGa                                                                            >  1:205059/1‑81 (MQ=255)       cccTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGATCGATTCTg                                                                       >  2:205050/1‑80 (MQ=255)         cTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTTCCTGGCGTCGCTGGTGGGATCGATTCTGaa                                                                     >  2:205063/1‑80 (MQ=255)            aCCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGATCGATTCTGAATACg                                                                 >  2:205065/1‑81 (MQ=255)             ccagccagCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGATCGATTCTGAATACGt                                                                >  1:205058/1‑81 (MQ=255)             ccagccagCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGATCGATTCTGAATACGt                                                                <  2:205033/81‑1 (MQ=255)                   agcTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGATCGATTCTGAATACGTGGAAcc                                                          <  2:205034/81‑1 (MQ=255)                   agcTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGATCGATTCTGAATACGTGGAAcc                                                          >  2:205061/1‑81 (MQ=255)                   agcTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGATCGATTCTGAATACGTGGAAcc                                                          <  2:205035/81‑1 (MQ=255)                            aGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGATCGATTCTGAATACGTGGAACCGCTTCTCGa                                                 >  2:205071/1‑81 (MQ=255)                            aGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGATCGATTCTGAATACGTGGAACCGCTTCTCGa                                                 >  1:205062/1‑81 (MQ=255)                                tACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGATCGATTCTGAATACGTGGAACCGCTTCTCGATTcc                                             >  2:205057/1‑81 (MQ=255)                                     ttCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGATCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGt                                        >  2:205069/1‑81 (MQ=255)                                       cccTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGATCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGtt                                       <  2:205036/80‑1 (MQ=255)                                                    tGATCTCCCTGGCGTCGCTGGTGGGATCGATTCTGAATACGTGGAACCGCTCCTCGATTTCGGCGTTTTCTCCAACACTGc                         >  1:910473/1‑81 (MQ=255)                                                      aTCTCCCTGGCGTCGCTGGTGGGATCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGc                         <  2:205037/79‑1 (MQ=255)                                                        ctcCCTGGCGTCGCTGGTGGGATCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTaa                     >  2:205068/1‑81 (MQ=255)                                                           ccTGGCGTCGCTGGTGTGATCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACAt                  <  1:205038/81‑1 (MQ=255)                                                             tGGCGTCGCTGGTGGGATCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACAt                  >  1:205079/1‑79 (MQ=255)                                                              ggCGTCGCTGGTGGGATCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAg               >  2:205085/1‑81 (MQ=255)                                                                    gCTGGTGGGATCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGAt         >  2:205064/1‑81 (MQ=255)                                                                     cTGGTGGGATCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGAtt        <  1:205039/81‑1 (MQ=255)                                                                         tggGATCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGtt    >  1:205073/1‑81 (MQ=255)                                                                           ggATCGATTCTGAATACGTGGAACCGCTTCTCGATCCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTc  >  1:205067/1‑81 (MQ=255)                                                                              |                                                                              AATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTC  >  NC_000913/1128206‑1128360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑