Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,129,774	T→G	K19N (AAA→AAC)	flgN ←	flagellar biosynthesis protein FlgN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,129,774	0	T	G	100.0%	73.7 / NA	22	K19N (AAA→AAC)	flgN	flagellar biosynthesis protein FlgN
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (8/14);  total (8/14)

GTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGGACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGT  >  NC_000913/1129698‑1129851                                                                             |                                                                              gTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTGTTg                                                                            <  2:205234/80‑1 (MQ=255)  ttCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTGTTgag                                                                          <  2:205235/81‑1 (MQ=255)           ccATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTGTTGAGATCGTTAAg                                                                 >  1:205268/1‑81 (MQ=255)              ttGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTGTTGAGATCGTTAAGca                                                               >  2:205269/1‑80 (MQ=255)                  aaCTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTGTTGAGATCGTTAAGCACAGCg                                                          >  1:205271/1‑81 (MQ=255)                       gCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTGTTGAGATCGTTAAGCACAGCGGACAt                                                     >  1:205264/1‑81 (MQ=255)                        cTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTGTTGAGATCGTTAAGCACAGCGGACATc                                                    <  1:205237/81‑1 (MQ=255)                        cTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTGTTGAGATCGTTAAGCACAGCGGACATc                                                    <  1:205236/81‑1 (MQ=255)                             gTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTGTTGAGATCGTTAAGCACAGCGGACATCTGGTc                                               <  2:205238/81‑1 (MQ=255)                                     gCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTGTTGAGATCGTTAAGCACAGCGGACATCTGGTCGAGGAtct                                       >  2:205272/1‑81 (MQ=255)                                      ccccATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTGTTGAGATCGTTAAGCACAGCGGACATCTGGTCGAGGAtctc                                      >  1:205275/1‑81 (MQ=255)                                      ccccATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTGTTGAGATCGTTAAGCACAGCGGACATCTGGTCGAGGAtctc                                      <  2:205239/81‑1 (MQ=255)                                             gagagaTGTTGCTGCTCTTGATCCATTACCGTGTTGAGATCGTTAAGCACAGCGGACATCTGGTCGAGGATCTCTGCAAGa                               >  2:205274/1‑81 (MQ=255)                                                 gaTGTTGCTGCTCTTGATCCATTACCGTGTTGAGATCGTTAAGCACAGCGGACATCTGGTCGAGGATCTCTGCAAGACgtg                           <  2:205240/81‑1 (MQ=255)                                                     ttGCTGCTCTTGATCCATTACCGTGTTGAGATCGTTAAGCACAGCGGACATCTGGTCGAGGATCTCTGCAAGACGTGTCa                        <  2:205241/80‑1 (MQ=255)                                                           ctctTGATCCATTACCGTGTTGAGATCGTTAAGCACAGCGGACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATc                 <  2:205242/81‑1 (MQ=255)                                                                gATCCATTACCGTGTTGAGATCGTTAAGCACAGCGGACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGtt             <  1:205243/80‑1 (MQ=255)                                                                gATCCATTACCGTGTTGAGATCGTTAAGCACAGCGGACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTa            >  2:205279/1‑81 (MQ=255)                                                                 aTCCATTACCGTGTTGAGATCGTTAAGCACAGCGGACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTAc           <  1:205244/81‑1 (MQ=255)                                                                  tCCATTACCGTGTTGAGATCGTTAAGCACAGCGGACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTAct          <  1:205245/81‑1 (MQ=255)                                                                      ttACCGTGTTGAGATCGTTAAGCACAGCGGACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGc      <  1:205246/81‑1 (MQ=255)                                                                          cGTGTTGAGATCGTTAAGCACAGCGGACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGt  <  1:205247/81‑1 (MQ=255)                                                                             |                                                                              GTTCTGTAATCCATTGCAACTGGCTGCCGTTGATCTGCCCCATAGAGAGATGTTGCTGCTCTTGATCCATTACCGTTTTGAGATCGTTAAGCACAGCGGACATCTGGTCGAGGATCTCTGCAAGACGTGTCATACGATCAGTTACTCTGCAAGT  >  NC_000913/1129698‑1129851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑