Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	141,845	G→A	V139I (GTC→ATC)	hpt →	hypoxanthine phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	141,845	0	G	A	100.0%	86.8 / NA	25	V139I (GTC→ATC)	hpt	hypoxanthine phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/12);  total (13/12)

AAGCCTGCGCGAACCGAAGTCGCTGGCGATTTGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCTCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGCGTTA  >  NC_000913/141775‑141921                                                                       |                                                                             aaGCCTGCGCGAACCGAAGTCGCTGGCGATTTGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACATCCCg                                                                         <  1:31220/76‑1 (MQ=255)        cgcgAACCGAAGTCGCTGGCGATTTGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACATCCCGGTAGAATTTATc                                                             >  1:31267/1‑81 (MQ=255)         gcgAACCGAAGTCGCTGGCGATTTGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACATCCCGGTAGAATTTATCg                                                            <  2:31221/81‑1 (MQ=255)                gAAGTCGCTGGCGATTTGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACATCCCGGTAGAATTTATCGGTTtctc                                                     <  1:31222/81‑1 (MQ=255)                     cGCTGGCGATTTGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACATCCCGGTAGAATTTATCGGTTTCTCGATcc                                                >  1:31273/1‑81 (MQ=255)                       cTGGCGATTTGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACATCCCGGTAGAATTTATCGGTTTCTCGATCCCg                                              >  2:31274/1‑81 (MQ=255)                         ggCGATTTGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACATCCCGGTAGAATTTATCGGTTTCTCGATCCCGGa                                            >  2:31268/1‑81 (MQ=255)                          gCGATTTGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACATCCCGGTAGAATTTATCGGTTTCTCGATCCCGGAt                                           <  1:31223/81‑1 (MQ=255)                             aTTTGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACATCCCGGTAGAATTTATCGGTTTCTCGATCCCGGATGAg                                        >  1:31260/1‑81 (MQ=255)                              tttGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACATCCCGGTAGAATTTATCGGTTTCTCGATCCCGGATGAGt                                       >  2:31270/1‑81 (MQ=255)                                    cGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACATCCCGGTAGAATTTATCGGTTTCTCGATCCCGGATGAGTTTgtgg                                 <  2:31224/81‑1 (MQ=255)                                         ctgGATAAACCGTCCCGTCGTGAAGTGAACATCCCGGTAGAATTTATCGGTTTCTCGATCCCGGATGAGTTTGTGGTGGGt                            <  1:31225/81‑1 (MQ=255)                                         ctgGATAAACCGTCCCGTCGTGAAGTGAACATCCCGGTAGAATTTATCGGTTTCTCGACCCCGGATGAGTTTGTGGTGGGt                            >  2:31289/1‑81 (MQ=255)                                         ctgGATAAACCGTCCCGTCGTGAAGTGAACAACCCGGTAGAATTTATCGGTTTCTCGATCCCGGATGAGTTTGTGGTGGGt                            <  2:31226/81‑1 (MQ=255)                                            gATAAACCGTCCCGTCGTGAAGTGAACATCCCGGTAGAATTTATCGGTTTCTCGATCCCGGATGAGTTTGTGGTGGGTTAc                         <  2:31227/81‑1 (MQ=255)                                            gATAAACCGTCCCGTCGTGAAGTGAACATCCCGGTAGAATTTATCGGTTTCTCGATCCCGGATGAGTTTGTGGTGGGTTAc                         >  2:31279/1‑81 (MQ=255)                                                 aCCGTCCCGTCGTGAAGTGAACATCCCGGTAGAATTTATCGGTTTCTCGATCCCGGATGAGTTTGTGGTGGGTTACGGCAt                    <  2:31228/81‑1 (MQ=255)                                                   cgtcccgtcGTGAAGTGAACATCCCGGTAGAATTTATCGGTTTCTCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTg                  <  1:31229/81‑1 (MQ=255)                                                   cgtcccgtcGTGAAGTGAACATCCCGGTAGAATTTATCGGTTTCTCGATCCCGGATGAGTTTGTGGAGGGTTACGGCAtt                   >  1:31284/1‑80 (MQ=255)                                                            gtgaagtgaaCATCCCGGTAGAATTTATCGGTTTCTCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGca          >  1:31290/1‑80 (MQ=255)                                                            gtgaagtgaaCATCCCGGTAGAATTTATCGGTTTCTCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGca          >  2:31277/1‑80 (MQ=255)                                                               aagtgagCATCCCGGTAGAATTTTTCGGTTTCTAGATCCCGGATGAGTTTGTGGTGGGTTACGACGTTGATTACGCACAGc      <  2:31231/81‑1 (MQ=255)                                                               aagtgaaCATCCCGGTAGAATTTATCGGTTTCTCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAg       <  1:31230/80‑1 (MQ=255)                                                                 gtgaaCATCCCGGTAGAATTTATCGGTTTCTCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGCGt    >  1:31299/1‑81 (MQ=255)                                                                   gaaCATCCCGGTAGAATTTATCGGTTTCTCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGCGTTa  >  1:31286/1‑81 (MQ=255)                                                                       |                                                                             AAGCCTGCGCGAACCGAAGTCGCTGGCGATTTGTACGCTGCTGGATAAACCGTCCCGTCGTGAAGTGAACGTCCCGGTAGAATTTATCGGTTTCTCGATCCCGGATGAGTTTGTGGTGGGTTACGGCATTGATTACGCACAGCGTTA  >  NC_000913/141775‑141921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑