Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,143,716	A→G	F218L (TTC→CTC)	rne ←	ribonuclease E

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,143,716	0	A	G	100.0%	74.3 / NA	22	F218L (TTC→CTC)	rne	ribonuclease E
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/13);  total (9/13)

GCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCTTTTTTGATGGCTT  >  NC_000913/1143641‑1143793                                                                            |                                                                              gCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAGTgcgc                                                                          >  2:207514/1‑81 (MQ=255)  cTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAGTgcgc                                                                          <  1:207484/80‑1 (MQ=255)        tGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAGTGCGCGAACGAt                                                                   >  1:207506/1‑81 (MQ=255)            aGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAGTGCGCGAACGATTACg                                                               >  2:207508/1‑81 (MQ=255)              ttCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAGTGCGCGAACGATTACGtt                                                             >  2:207521/1‑81 (MQ=255)                   gCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAGTGCGCGAACGATTACGTTGctct                                                        <  1:207485/81‑1 (MQ=255)                   gCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAGTGCGCGAACGATTACGTTGctct                                                        <  1:207486/81‑1 (MQ=255)                      cTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAGTGCGCGAACGATTACGTTGCTCTCCt                                                     <  1:207488/81‑1 (MQ=255)                      cTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAGTGCGCGAACGATTACGTTGCTCTCCt                                                     <  1:207487/81‑1 (MQ=255)                           gggTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAGTGCGCGAACGATTACGTTGCTCTCCtgatga                                                <  1:207489/81‑1 (MQ=255)                               tATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAGTGCGCGAACGATTACGTTGCTCTCCTGATGAAt                                              >  2:207523/1‑79 (MQ=255)                                aTCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAGTGCGCGAACGATTACGTTGCTCTCCTGATGAATCAg                                           >  1:207513/1‑81 (MQ=255)                                   gATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAGTGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGa                                         <  2:207490/80‑1 (MQ=255)                                               gCCGATGTCCTGACGTAAGTAATCGCGGAGTGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGggcg                            <  1:207492/81‑1 (MQ=255)                                               gCCGATGTCCTGACGTAAGTAATCGCGGAGTGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGggc                             <  2:207493/80‑1 (MQ=255)                                               gCCGATGTCCTGACGTAAGTAATCGCGGAGTGCGCGAACGACTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGggc                             <  1:207491/80‑1 (MQ=255)                                                      tCCTGACGTAAGTAATCGCGGAGTGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCttt                       >  2:207515/1‑79 (MQ=255)                                                           aCGTAAGTAATCGCGGAGTGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGc                <  2:207494/81‑1 (MQ=255)                                                              taagtaaTCGCGGAGTGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCttt             >  1:207518/1‑81 (MQ=255)                                                                 gtaaTCGCGGAGTGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCtttttt          <  2:207495/81‑1 (MQ=255)                                                                       gcgGAGTGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCTTTTTTGATGGc    >  2:207530/1‑81 (MQ=255)                                                                         ggAGTGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCTTTTTTGATGGCtt  <  1:207496/81‑1 (MQ=255)                                                                            |                                                                              GCTGACGTGCCAGTTCGAGCACTTTCGGGTTATCGATAAGGATTTCGCCGATGTCCTGACGTAAGTAATCGCGGAATGCGCGAACGATTACGTTGCTCTCCTGATGAATCAGGAACGGGGCCGGGCGGCTTTCAGCGGCTTTTTTGATGGCTT  >  NC_000913/1143641‑1143793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑