Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,146,195	T→C	E134G (GAG→GGG)	yceF ←	m(7)GTP pyrophosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,146,195	0	T	C	100.0%	84.9 / NA	25	E134G (GAG→GGG)	yceF	m(7)GTP pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/15);  total (10/15)

AGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATT  >  NC_000913/1146117‑1146268                                                                               |                                                                          aGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATcccc                                                                         >  2:207864/1‑81 (MQ=255) aGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATcccc                                                                         <  2:207823/81‑1 (MQ=255)   cGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCCCCGc                                                                       <  1:207824/81‑1 (MQ=255)   cGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCCCCGc                                                                       >  1:207853/1‑81 (MQ=255)    gTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCCCCGcc                                                                      >  2:207857/1‑81 (MQ=255)    gTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCCCCGcc                                                                      >  1:207848/1‑81 (MQ=255)       aTGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCCCCGCCTCg                                                                   <  1:207825/81‑1 (MQ=255)              aTCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCCCCGCCTCGCTCAGGt                                                            <  1:207826/81‑1 (MQ=255)              aTCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCCCCGCCTCGCTCAGGt                                                            >  1:207850/1‑81 (MQ=255)                    cACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCCCCGCCTCGCTCAGGTGACgg                                                       >  2:207854/1‑80 (MQ=255)                           aaaGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCCCCGCCTCGCTCAGGTGACGGAAATgg                                                 <  1:207827/79‑1 (MQ=255)                           aaaGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCCCCGCCTCGCTCAGGTGACGGAAATGGAc                                               >  1:207859/1‑81 (MQ=255)                              gCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCCCCGCCTCGCTCAGGTGACGGAAATGGACGTc                                            <  1:207828/81‑1 (MQ=255)                                   ccGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCCCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAg                                       <  1:207829/81‑1 (MQ=255)                                     gcgcAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCCCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGc                                     <  1:207830/81‑1 (MQ=255)                                       gcAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCCCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTc                                   <  1:207831/81‑1 (MQ=255)                                         aGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCCCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCa                                 <  1:207832/81‑1 (MQ=255)                                          gTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCCCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCAc                                >  1:207861/1‑81 (MQ=255)                                              aGGGGATGCTCTTTACGCACGTAATTATCAATCCCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCt                            <  2:207833/81‑1 (MQ=255)                                                    tGCTCTTTACGCACGTAATTATCAATCCCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGc                      <  1:207834/81‑1 (MQ=255)                                                     gCTCTTTACGCACGTAATTATCAATCCCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTg                       >  2:207865/1‑79 (MQ=255)                                                           tACGCACGTAATTATCAATCCCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGAt                  <  2:207835/78‑1 (MQ=255)                                                              gCACGTAATTATCAATCCCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCAt            <  2:207836/81‑1 (MQ=255)                                                                      ttATCAATCCCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGaa    <  1:207837/81‑1 (MQ=255)                                                                      ttATCAATCCCCGCCTCGCGCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGaa    >  2:207866/1‑81 (MQ=255)                                                                        aTCAATCCCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAAt   <  2:207838/80‑1 (MQ=255)                                                                         tCAATCCCCGCCTCGCTCAGGTGACGGAAATAGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAAtt  >  2:207868/1‑80 (MQ=255)                                                                               |                                                                          AGCGTAATGCCAAATCCTTCACTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATT  >  NC_000913/1146117‑1146268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑