Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,149,850	C→T	G41G (GGC→GGT)	fabD →	[acyl‑carrier‑protein] S‑malonyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,149,850	0	C	T	100.0%	99.9 / NA	28	G41G (GGC→GGT)	fabD	[acyl‑carrier‑protein] S‑malonyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/4);  total (24/4)

TGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGACCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAACCTGGCAAACTCAGCCTGCGCT  >  NC_000913/1149775‑1149924                                                                            |                                                                           tGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGTTACGa                                                                       >  2:208424/1‑81 (MQ=255)  ggAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGTTACGAc                                                                      >  2:208434/1‑81 (MQ=255)      tGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGTTACGACCtgt                                                                  <  2:208396/81‑1 (MQ=255)        ctggctgATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGTTACGACCTGTgg                                                                >  2:208432/1‑81 (MQ=255)         tggctgATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGATGCTTCTGCGGCGCTGGGTTACGACCTGTggg                                                               >  2:208438/1‑81 (MQ=255)         tggctgATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGTTACGACCTGTggg                                                               >  2:208425/1‑81 (MQ=255)          ggctgATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGTTACGACCTGTGGgc                                                              >  1:208414/1‑81 (MQ=255)                tatGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGTTACGACCTGTGGGCGCTGAc                                                        >  1:208430/1‑81 (MQ=255)                 atGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGTTACGACCTGTGGGCGCTGAcc                                                       >  1:208439/1‑81 (MQ=255)                      ggcgAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGTTACGACCTGTGGGCGCTGACCcag                                                    >  1:208450/1‑79 (MQ=255)                              aTCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGTTACGACCTGTGGGCGCTGACCCAGCAGGGGCCAg                                          >  2:208426/1‑81 (MQ=255)                                     tGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGTTACGACCTGTGGGCGCTGACCCAGCAGGGGCCAGCTgaag                                    >  1:208442/1‑80 (MQ=255)                                      gTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGTTACGACCTGTGGGCGCTGACCCAGCAGGGGCCAGCTgaagaa                                  >  2:208431/1‑81 (MQ=255)                                         gaagaaACGTTTGCTGAAGCTTCTGCGGCGCTGGGTTACGACCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACt                                >  2:208449/1‑80 (MQ=255)                                         gaagaaACGTTTGCTGAAGCTTCTGCGGCGCTGGGTTACGACCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACt                                >  1:208436/1‑80 (MQ=255)                                          aagaaACGTTTGCTGAAGCTTCTGCGGCGCTGGGTTACGACCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTg                               >  1:208429/1‑80 (MQ=255)                                             aaaCGTTTGCTGAAGCTTCTGCGGCGCTGGGTTACGACCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATa                           <  1:208397/81‑1 (MQ=255)                                               aCGTTTGCTGAAGCTTCTGCGGCGCTGGGTTACGACCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATaa                          >  2:208452/1‑80 (MQ=255)                                                     gCTGAAGCTTCTGCGGCGCTGGGTTACGACCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAACCTgg                   >  2:208440/1‑81 (MQ=255)                                                     gCTGAAGCTTCTGCGGCGCTGGGTTACGACCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAACCTg                    <  1:208398/80‑1 (MQ=255)                                                            cTTCTGCGGCGCTGGGTTACGACCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAACCTGGCAAACt             >  1:208441/1‑80 (MQ=255)                                                               cTGCGGCGCTGGGTTACGACCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAACCTGGCAAACTCAGc         >  1:208465/1‑81 (MQ=255)                                                               cTGCGGCGCTGGGTTACGACCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAACCTGGCAAACTCAGc         >  1:208437/1‑81 (MQ=255)                                                               cTGCGGCGCTGGGTTACGACCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAACCTGGCAAACTCAGc         >  2:208435/1‑81 (MQ=255)                                                               cTGCGGCGCTGGGTTACGACCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAACCTGGCAAACTCAGc         >  2:208447/1‑81 (MQ=255)                                                                 gcggcgCTGGGTTACGACCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAACCTGGCAAACTCAGCCt       >  2:208467/1‑81 (MQ=255)                                                                   ggcgCTGGGTTACGACCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAACCTGGCAAACTCAGCCTgc     >  1:208446/1‑81 (MQ=255)                                                                      gcTGGGTTACGACCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAACCTGGCAAACTCAGCCTGCGCt  <  2:208399/81‑1 (MQ=255)                                                                            |                                                                           TGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGACCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAACCTGGCAAACTCAGCCTGCGCT  >  NC_000913/1149775‑1149924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑