Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,150,676	T→C	F3L (TTT→CTT)	fabG →	3‑oxoacyl‑[acyl‑carrier‑protein] reductase FabG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,150,676	0	T	C	100.0%	94.6 / NA	28	F3L (TTT→CTT)	fabG	3‑oxoacyl‑[acyl‑carrier‑protein] reductase FabG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/7);  total (21/7)

ACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCCGCGCAATTGCTGAAACGCTCGCAGCCCGT  >  NC_000913/1150598‑1150753                                                                               |                                                                              acCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAAtctt                                                                             <  2:208549/81‑1 (MQ=255)   ccTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATCTTGa                                                                           <  2:208550/81‑1 (MQ=255)      gACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATCTTGAAg                                                                         >  2:208581/1‑80 (MQ=255)      gACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATCTTGAAg                                                                         >  2:208574/1‑80 (MQ=255)        ccGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATCTTGAAGGaa                                                                      <  1:208551/81‑1 (MQ=255)           ccTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATCTTGAAGGaaaaa                                                                   >  2:208594/1‑81 (MQ=255)            cTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATCTTGAAGGAAAAAt                                                                  >  2:208576/1‑81 (MQ=255)                      aacgaacCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATCTTGAAGGAAAAATCGCACTGGt                                                         >  2:208577/1‑80 (MQ=255)                      aacgaacCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATCTTGAAGGAAAAATCGCACTGGTa                                                        <  1:208552/81‑1 (MQ=255)                          aacCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATCTTGAAGGAAAAATCGCACTGGTAACCg                                                    <  2:208553/81‑1 (MQ=255)                           acCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATCTTGAAGGAAAAATCGCACTGGTAACCgg                                                   >  1:208566/1‑81 (MQ=255)                           acCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATCTTGAAGGAAAAATCGCACTGGTAACCgg                                                   >  1:208573/1‑81 (MQ=255)                            ccTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATCTTGAAGGAAAAATCGCACTGGTAACCGGt                                                  >  2:208583/1‑81 (MQ=255)                             cTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATCTTGAAGGAAAAATCGCACTGGTAACCGGTg                                                 >  2:208591/1‑81 (MQ=255)                             cTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATCTTGAAGGAAAAATCGCACTGGTAACCGGTg                                                 >  2:208560/1‑81 (MQ=255)                                  gCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATCTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGc                                            >  1:208600/1‑81 (MQ=255)                                   cGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATCTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGcc                                           <  2:208554/81‑1 (MQ=255)                                            cggcgCTCGAGCTTTAAAAGAGGAAAATCATGAATCTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTg                                  >  1:208575/1‑81 (MQ=255)                                             ggcgCTCGAGCTTTAAAAGAGGAAAATCATGAATCTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTgg                                 >  2:208592/1‑81 (MQ=255)                                              gcgcTCGAGCTTTAAAAGAGGAAAATCATGAATCTTGAAGGAAAAATCGTACTGGTAACCGGTGCAAGCCGCGGAATTGGc                                >  2:208593/1‑81 (MQ=255)                                               cgcTCGAGCTTTAAAAGAGGAAAATCATGAATCTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGcc                               >  2:208585/1‑81 (MQ=255)                                                gcTCGAGCTTTAAAAGAGGAAAATCATGAATCTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCcg                              >  1:208579/1‑81 (MQ=255)                                                    gAGCTTTAAAAGAGGAAAATCATGAATCTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCCgcgc                           >  2:208588/1‑80 (MQ=255)                                                       cTTTAAAAGAGGAAAATCATGAATCTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCCGCGCAAtt                       <  1:208555/81‑1 (MQ=255)                                                            aaaGAGGAAAATCATGAATCTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCCGCGCAATTGCTg                   >  2:208582/1‑80 (MQ=255)                                                                agGAAAATCATGAATCTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCCGCGCAATTGCTGAAACg              >  1:208596/1‑81 (MQ=255)                                                                    aaaTCATGAATCTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCCGCGCAATTGCTGAAAcgcgcg          >  2:208601/1‑81 (MQ=255)                                                                     aaTCATGAATCTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCCGCGCAATTGCTGAAACGCTCGc         <  2:710506/81‑1 (MQ=255)                                                                            aaTCTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCCGCGCAATTGCTGAAACGCTCGCAGCCCGt  >  2:208589/1‑81 (MQ=255)                                                                               |                                                                              ACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCCGCGCAATTGCTGAAACGCTCGCAGCCCGT  >  NC_000913/1150598‑1150753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑